Search PubMed⌕ Search

Biomedical subjects

H T Chen

Publications and source records attributed to H T Chen.

96 records · Page 6Linked to original sources
PubMedDate not supplied

An experimental transmission of woodchuck hepatitis virus to young Chinese marmots.

Fourteen young Chinese marmots (Marmota bobak sibirica Radde) were randomly allocated to two groups of seven each. They were injected intrahepatically with a standard woodchuck hepatitis virus challenge pool or a negative pool, prepared from sera of woodchucks with and without woodchuck hepatitis virus infection, respectively. Marmot No. 2 in the experimental group experienced an episode of seroconversion from woodchuck hepatitis surface antigen to anti-woodchuck hepatitis antibody. Woodchuck hepatitis virus DNA was detected, and woodchuck hepatitis surface antigen particles of both spherical and filamentous forms and intact woodchuck hepatitis virus virions were found in its serum. By contrast, none of the control group animals (receiving the negative pool) produced any marker of woodchuck hepatitis virus. This suggests that young Chinese marmots can get woodchuck hepatitis virus infection.

Animals↗
PubMedDate not supplied

Experience of 73 free groin flaps.

The free groin flap has gradually lost its popularity due to the disadvantages relating to the pedicle and the plethora of new donor sites. The purpose of this article is to re-evaluate this time-honoured free flap and to demonstrate its reliability and versatility through a review of 73 free groin flaps performed between 1985 and 1990. 7% of free transfers (total 1096 cases) in our institution were free groin flaps. The complication rate was 8% (three complete failures and three partial failures). This result is acceptable and not so high as other reports have described. The artery discrepancy is the main cause of the complication rate. Two special techniques to gain additional diameter of vessel size to overcome the pedicle disadvantages are stressed. The groin flap has proved to be useful and still deserves consideration in reconstructive procedures.

Adolescent↗
PubMedDate not supplied

Acute colonic intramural hematoma due to blunt abdominal trauma.

Trauma to the colon is uncommon and accounts for only 3-5% of all blunt abdominal injuries. Among them, intramural hematoma of the colon is a rare complication and the acute form is rarer than the chronic form. We report a 37-year-old man who presented with abdominal pain followed by intestinal obstruction due to a blunt trauma. The initial diagnosis was done by sonography and proved by computed tomography (CT). Abdominal sonography also detected an increment in the size of the hematoma with progressive abdominal cramping pain that prompted urgent laparotomy. Ileocolic segmental resection with end-to-end ileocolostomy was performed and the patient recovered uneventfully. Based on our experience with a patient suffering from an intramural colonic hematoma following blunt abdominal trauma (BAT) and based on a review of the literature, we discuss the different clinical manifestations, difficulties of diagnosis, and different treatment modalities of this disease entity. We conclude that acute colonic hematoma can be diagnosed by sonography and/or CT in contrast to the early reported cases, in the pre-CT era, when they could only be diagnosed at laparotomy. Endoscopy may also be helpful for diagnosis in some cases. Although expectant therapy may be successful in some cases, the majority of the cases may need operation.

Abdominal Injuries↗
PubMedDate not supplied

Farnesol for aerosol inhalation: nebulization and activity against human lung cancer cells.

PURPOSE: A nebulized aerosol formulation of the anti-cancer agent farnesol is developed and shown to induce cell death of human lung cancer cells in vitro. METHODS: A nebulized farnesol formulation containing polysorbate 80 (Tween 80) is developed. The measurements of the aerosol properties during nebulization were used as input for a mathematical model of airway surface liquid in the lung of an average adult, to estimate the airway surface liquid drug concentration of the deposited farnesol. Cytotoxicity of the formulations was measured in vitro on non-small cell lung cancer cells (H460 and A549). RESULTS: As much as 100% of lung cancer cytotoxicity can be achieved by using Pari LC Star and LC Plus nebulizers. The estimated airway surface liquid concentrations of the deposited farnesol reveal that the IC50 of the nebulized farnesol can be achieved over the entire tracheobronchial region, using the above Pari nebulizers with a volume fill of 5 ml. CONCLUSIONS: Drug concentrations higher than IC50 in the airway surface liquid are predicted with our methods, suggesting in vivo trials of a formulation may be warranted with these particular nebulizers.

Administration, Inhalation↗