Biomedical subjects

H Suginami

Publications and source records attributed to H Suginami.

61 records · Page 4Linked to original sources

PubMed1976

In vitro steroidogenesis by human corpora lutea of pregnancy. Effects of human chorionic gonadotropin and prostaglandin F2alpha.

Human corpora lutea of pregnancy obtained at 5 to 11 weeks of gestation were incubated in vitro in the presence of various concentrations of human chorionic gonadotropin (hCG) and prostaglandin F2alpha (PGF2alpha). Progesterone (P) and 17beta-estradiol (E2) released into the medium during incubation were measured by radioimmunoassay. Pieces of the corpora lutea were also examined ultrastructurally before and after incubation. Release of P and E2 into the medium was significantly increased by the addition of hCG and PGF2alpha in some cases. However, the response to hCG and PGF2alpha appeared to vary according to the age of the corpus luteum. Ultrastructurally, the lutein cells were well maintained with respect to the appearance of the smooth endoplasmic reticulum and mitochondria after 120-min incubations. On the basis of these observations, the following conclusions have been reached: 1) This incubation system seems to be suitable for investigating the direct effects of chemicals on lutein cells in vitro. 2) Both hCG and PFG2alpha directly stimulate steroidogenesis in the human corpus luteum of pregnancy, demonstrating their luteotropic actions in vitro. 3) Observed variations in P and E2 secretion might have been due to the different activities of aromatizing enzymes at different stages of gestation.

Chorionic Gonadotropin↗

PubMed1975

[Composite microassays of plasma progesterone, 17alpha-OH-progestrerone, estrone, 17beta-estradiol and estriol in normal adult women. I. Assay methods and steroid patterns in normal menstrual cycle (author's transl)].

Composite microassays for plasma progesterone (P), 17alpha-OH-progesterone (17P), estrone (E1), 17beta-estradiol (E2), and estriol (E3) were developed. Steroids were extracted from plasma samples with diethyl ether, and were separated from each other through two steps of sephadex LH-20 microcolumn chromatography (benzene:methanol 85:15, and n-hexane: benzene: methanol 80:10:10), prior to assays by radioimmunoassay (P, E1, E1, and E3) and competitive protein binding assay (17P). Steroids were recovered satisfactorily through these procedures (mean recovery; P:107.6%, 17P:102.3%, E1:88.2%, E2:84.1%. E3:78.5%). The detectable range for P, 17P, E1, E2, and E3 were 0.01-2 ng/tube, 0.1-10 ng/tube, 10-2000pg/tube, 10-2000 pg/tube, and 0.5-100 ng/tube. The interassay coefficient of variations were less than 10.7%, 15.2%, 17.8%, 11.4%, and 28.1%, respectively. These steroids were measured daily in 4 menstrual cycles from 4 normally menstruating women. Plasma FSH and LH were quantitated previously. The following results were obtained; 1) Plasma P elevated from around LH surge (Day O), and reached a peak on day +5 approximately +7 with the values of 12.74 +/- 2.34 ng/ml (Mean +/- S.D.). 2) Slight decrease in P levels was noted on day +8 approximately +9. 3) A peak in 17P was observed in the preovulatory phase (day -3 approximately -1) with the values of 0.6 approximately 1.3 ng/ml in three of four cases. 4) Changes of 17P during the luteal phase were paralleled to those of P with a peak of 1.16 +/- 0.31 ng/ml on day +5 approximately +7. 5) No remarkable patterns were found in E1 levels throughout the menstrual cycle. 6) A sharp peak in E2 was detected in the preovulatory phase (day -1 approximately 0) with the values of 709.2 +/- 95.9 pg/ml. 7) The second peak of E2 with 378.6 +/- 140.7 pg/ml was observed in the late luteal phase (day +8 approximately +12). 8)E3 was not detected in all samples. The interrelationship between steroids and the correlation with the morphological changes of the ovaries in the normal menstrual cycle are discussed. In the follicular phase, the theca interna cells around the maturing follicle may be growing under the influence of pitiutary gonadotropins to secrete large amounts of 17P and E2, which may possibly affect the pituitary for LH surge, followed by ovulation. In the luteal phase, both the granulosa cells and theca intera cells are luteinized, which may produce and secrete large amount of P, 17P, and E2.

Estradiol↗

PubMed1975

[Composite microassays of plasma progesterone, 17alpha-OH-progesterone, estrone, 17beta-estradiol and estriol in normal adult women. II.Steroid patterns in normal pregnancy, labor and the puerperium (author's transl)].

Plasma progesterone, 17alpha-OH-progesterone, estrone, 17beta-estradiol and estriol in human normal pregnancy, labor and the puerperium were measured simultaneously with radioimmunoassay or competitive protein binding assay. The steroids were extracted from the sample plasma with diethyl either, and were separated through two steps of sephadex LH-20 microcolumn chromatography prior to the assays. From the beginning to the 25th week of pregnancy, a gradual rise in the levels of plasma progesterone was noted, followed by a steep increase toward term with a level of 150-250 ng/ml at the end of gestation. The levels of 17alpha-OH-progesterone during the 7th or 8th week of pregnancy were 2-3 ng/ml, which was above the normal range in the luteal phase, followed by a gradual decrease during 15-25 weeks of gestation. Thereafter, an increase toward term was seen with a mean level of 6-10 ng/ml in the 40th week. From the beginning to the end of pregnancy, plasma levels of estrone, 17beta-estradiol, and estriol increased from less than 0.5 ng/ml to 2-4 ng/ml, from 1-2.5 ng/ml to 6-10 ng/ml, and from less than 1 ng/ml to 6-10 ng/ml, respectively. The disappearance of steroids from maternal peripheral blood in the puerperium was rapid. The half time of progesterone, 17alpha-OH-progesterone, estrone, 17beta-estradiol, and estriol were 72 min, 68 min, 27 min, 27 min, and 48 min, respectively. Analysis of the interrelation between several kinds of steroids of pregnancy, labor, and the puerperium with simultaneous microassays will contribute to the clarification of some important physiological and pathological aspects of feto-placental-maternal functions and steroidogenic functions of the corpus luteum in pregnancy.

Estradiol↗

PubMed1974