Search PubMed⌕ Search

Biomedical subjects

H Sueki

Publications and source records attributed to H Sueki.

46 records · Page 3Linked to original sources
PubMed1991

[Increased corneocyte surface area in the diabetic skin].

In attempt to prove the hypothesis that corneocyte surface area in diabetics is larger than that in age-matched non-diabetics, we collected corneocytes from 50 diabetics and 43 non-diabetics in different decades of life. We measured surface areas of 27 +/- 3 corneocytes in each subject, utilizing an image analyser (Kontron MOP-10), then we counted mean number of corneocytes collected in 7 diabetics and 7 non-diabetics. There was a significant correlation between corneocyte surface area and chronological age in non-diabetics, whereas there was no significant correlation in diabetics. Corneocyte surface area of diabetics was larger than that of non-diabetics in every decades of life. The differences were statistically significant in 4th, 7th and 8th decades of life. Mean number of corneocytes collected in non-diabetics was 2.21 +/- 0.47 (X 10(5)), whereas that in diabetics was 2.49 +/- 0.55 (X 10(5)). The present study suggested that turnover time of corneal layer of the skin might be delayed in diabetics.

Adult↗
PubMed1989

Glycosylated proteins of skin, nail and hair: application as an index for long-term control of diabetes mellitus.

The purpose of the present study was to compare the degrees of nonenzymatically glycosylated proteins in the skin (stratum corneum), the nail, the hair, and hemoglobin obtained simultaneously from the same subject and to evaluate the most useful sample for management of diabetic complications. Fifty-one diabetic patients and 20 control patients were examined, utilizing furosine determination. Furosine value of the skin in diabetics was 2.14 +/- 1.70%, whereas that in controls was 1.65 +/- 0.47%. Furosine value of the nail in diabetics was 6.67 +/- 3.30%, whereas that in controls was 4.16 +/- 1.62%. Furosine value of the hair in diabetics was 1.30 +/- 1.11%, whereas that in controls was 1.29 +/- 1.71%. Close correlations were detected between HbA1 (glycosylated hemoglobin) and furosine of the nail (r = 0.58, p less than 0.001), HbA1 and furosine of the skin (r = 0.48, p less than 0.001), and HbA1 and furosine of the hair (r = 0.43, p less than 0.01); however, poor correlations were found between furosine of the hair and the skin (r = 0.35, p less than 0.05) and furosine of the nail and the hair (r = 0.33, p less than 0.05). Furosine of the nail was significantly correlated with the FBS (fasting blood sugar) of the same time, previous 6, and previous 12 months. Furosine value of the nail, we believe, is the most useful indicator for evaluating long term control of diabetics and may provide useful information for management of diabetic complications.

Adult↗
PubMed1987

Eccrine spiradenoma. An electron microscopic study.

Electron-microscopic studies of two typical lesions of eccrine spiradenoma were performed to correlate ultrastructure and histopathology. The salient ultramorphological features of the parenchyma were an adenoid cystic organization composed of epithelial, myoepithelial, and nonepithelial cell types, and the presence of intracytoplasmic luminae within the epithelial cells. There were no indications that the parenchyma was secretorily active. The stroma ramified through the parenchyma, occupying extensive areas and forming tenuous septa of the loose connective tissue in which blood vessels and nerve fibers were embedded. Profiles of cystoid spaces resulting from invagination of stroma into the parenchyma were frequently encountered. Based on our observations, eccrine spiradenoma appears to differentiate toward ductal structures of eccrine sweat apparatus.

Adenoma, Sweat Gland↗
PubMed1987

Diabetic microangiopathy in subcutaneous fatty tissue.

To evaluate diabetic microangiopathy in the subcutaneous fatty tissue objectively and to clarify the relation between pathogenesis of membranocystic lesion (MCL) and diabetic microangiopathy, specimens obtained from 23 diabetics and 23 nondiabetics were examined histologically. Ten of 23 diabetics and 7 of 23 non-diabetics were examined electron microscopically. Using electron micrographs measurements were made of the following areas; entire microvessel section, basal laminae, luminal space, endothelial cells, pericytes, and we scored the following findings: veil cells, cellular debris and vacuoles in the thickened basal laminae, abnormal densities of the endothelial cells. In diabetics, the area of luminal space was smaller and the area of basal laminae was larger than those of non-diabetics. Scored assessment of the veil cells, cellular debris and vacuoles in the thickened basal laminae were statistically significant in diabetics. Veil cells around the subcutaneous microvessels were less frequent and possessed fewer cytoplasmic processes than those around the dermal microvessels. Histologically, MCLs were frequently demonstrated in skin disorders resulting from diabetic microangiopathy, including 3 cases of pigmented pretibial patches, and 1 case of diabetic bulla. MCLs were more frequently demonstrated in diabetic cases with retinopathy, neuropathy, and nephropathy than those without complications. MCLs were detected only on the shins and the feet. MCLs were more frequently seen in cases with larger area of basal laminae than those with smaller area of basal laminae in morphometric measurement of electron micrographs. The present studies suggest that microangiopathy in the subcutaneous tissue is a pathological feature of diabetes mellitus and is a contributory factor to the formation of MCL.

Adipose Tissue↗
PubMed1986

Ultrastructural study of the histogenesis of membranocystic lesions (Nasu) in diabetics.

In order to clarify the histogenesis of membranocystic lesion (MCL) of the skin, 14 biopsies obtained from the shins and feet of diabetics were examined by light and electron microscopy. MCL was observed in 10 of 14 cases, including 7 pigmented pretibial patches (PPP), each a case of diabetic bulla, callus of the knee, and ordinary psoriasis. Ultrastructurally, MCL was characterized by 3 fundamental types of structure: tortuous thick bands composed of well-developed minute tubular structures; shrubbery-like structures in sectional profile consisting of accumulated tiny cysts and microprojections; and thin membranes without minute tubular structures. The small vessels in the dermis and subcutaneous tissue indicated the changes of microangiopathy. Present observations suggested that MCL was derived from subcutaneous fat cells and was displaced into the dermis to be disposed by histiocytes. Circulatory disturbance due to diabetic microangiopathy in the subcutaneous tissue could be one of the contributory factors in the formation of MCL.

Aged↗
PubMed1984

Immunohistochemical and electron microscopic observations of nevic corpuscle.

Nevic corpuscle in the pigmented nevus was studied under light microscopy in comparison with Meissner corpuscle of the human sole skin in order to determine its ontogenesis. No nerve fibers within nevic corpuscle but coiled fibers in Meissner corpuscle could be demonstrated by Bodian stain. Presence of S-100 protein in the pigmented nevus and Meissner corpuscle was observed under light microscopy utilizing peroxidase-antiperoxidase immunohistochemical staining. S-100 protein was more evident in nevic corpuscles than in Types A and B intradermal nevus cells. Meissner corpuscle showed moderate stainability for S-100 protein. Ultrastructural study revealed that the nevic corpuscle was composed of the laminated cells with flattened cytoplasmic processes but lacked peripheral nerve endings such as axons, characteristically shown in Meissner corpuscle. No melanogenic activity of nevic corpuscle was observed. These morphological and histochemical findings of nevic corpuscle did not enable us to give definite conclusion on its ontogenesis.

Female↗
PubMedDate not supplied

Hairless guinea pig skin: anatomical basis for studies of cutaneous biology.

In order to characterize the microscopic anatomy of hairless guinea pig (HL-GP) skin, we utilized light microscopy with a computer-assisted image analysis system, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM revealed that the hair shafts of HL-GPs were thin, short, extremely irregular in diameter and often twisted and curled. The HL-GP epidermis was of similar thickness to that of human skin with distinct strata, serrated/non-serrated basal keratinocytes and shallow dermal papillae. The density of Langerhans cells in epidermal sheets, visualized by adenosine-s-triphosphatase staining, was similar to that of normal-haired guinea pigs (HD-GPs), although the dendrites of HL-GPs were thicker and shorter than those of HD-GPs. The dermal vasculature of HL-GPs was well-developed and similar to that of humans, demonstrating a network of vertically oriented capillary loops. HL-GPs had significantly more dendritic or spindle-shaped dermal interstitial cells than humans and HD-GPs. Collectively, these data suggest that HL-GP skin is more similar to human skin than to the skin of HD-GPs and other rodents and, therefore, the HL-GP may be a useful animal for studying cutaneous biology, experimental pathology, pharmacology and toxicology.

Animals↗