Search PubMed⌕ Search

Biomedical subjects

H Su

Publications and source records attributed to H Su.

153 records · Page 9Linked to original sources
PubMed1984

High tension electrical burns of upper arms treated by segmental excision of necrosed humerus. An introduction of a new surgical method.

This paper describes treating high-tension electrical burns of the upper arms by segmental excision of the necrosed humerus. This is a new surgical method of treating local electrical burns. The authors use modification of traditional surgical method, shorten the course of treatment, and preserve the injured limb. However, this surgical method should not be used to treat all high-tension electrical burns of the upper arm, and should be used only under certain conditions. Therefore, it is important to grasp the operative indications.

Adult↗
PubMed1980

Studies on the ultrastructure of envelope of elementary bodies of Chlamydia trachomatis.

Electron microscopy of purified Chlamydia trachomatis treated by various physical and chemical means shows that the envelope of the elementary bodies (EB) consists of the outer cell wall layer, the inner cell wall layer, and the inner membrane. The outer cell wall layer is mainly made up of granular subunits arranged in a hexagonal pattern and may be called the "mural structural protein layer". It forms together with the inner cell wall layer the rigid cell wall. The inner or protoplasmic membrane is not easily shown in the young EB. In the negatively stained specimen, a frail and easily removable superficial layer is seen adhering to the "mural structural protein layer". But in ultra-thin sections this superficial layer is not shown separately. There are fold-like grooves which are special structures probably related to reproduction. There are seen some EB's in binary fission, and a number of bodies very big in size yet having morphological characteristics of the EB's, which are in various processes of multiplication, such as binary fission, budding, and "multicentered germination". These observations give a challenge to the current opinion that only the initial bodies have the power of reproduction.

Cell Membrane↗
PubMedDate not supplied

A hypoxia-regulated adeno-associated virus vector for cancer-specific gene therapy.

The presence of hypoxic cells in human brain tumors is an important factor leading to resistance to radiation therapy. However, this physiological difference between normal tissues and tumors also provides the potential for designing cancer-specific gene therapy. We compared the increase of gene expression under anoxia (<0.01% oxygen) produced by 3, 6, and 9 copies of hypoxia-responsive elements (HRE) from the erythropoietin gene (Epo), which are activated through the transcriptional complex hypoxia-inducible factor 1 (HIF-1). Under anoxic conditions, nine copies of HRE (9XHRE) yielded 27- to 37-fold of increased gene expression in U-251 MG and U-87 MG human brain tumor cell lines. Under the less hypoxic conditions of 0.3% and 1% oxygen, gene activation by 9XHRE increased expression 11- to 18-fold in these cell lines. To generate a recombinant adeno-associated virus (rAAV) in which the transgene can be regulated by hypoxia, we inserted the DNA fragment containing 9XHRE and the LacZ reporter gene into an AAV vector. Under anoxic conditions, this vector produced 79- to 110-fold increase in gene expression. We believe this hypoxia-regulated rAAV vector will provide a useful delivery vehicle for cancer-specific gene therapy.

Adenoviridae↗
PubMedDate not supplied

[Tetanus in intensive care units].

INTRODUCTION: Tetanus is medical disease with a high mortality rate, even in high tech centres and in Intensive Care Units (ICU). AIMS. To analyse the appearance and evolution of tetanus in the ICU at our hospital. PATIENTS AND METHODS: This retrospective descriptive study, made up of 26 patients admitted to hospital with tetanus in the ICU at the Hospital Escuela during the period between January 1995 and December 2001, examined the clinico epidemiological of the disease and the clinical evolution of the patients. RESULTS: Of the cases reviewed (n= 26), 34.6% were females and 65.4% males. The main clinical manifestations were: trismus (88%), dysphagia (77%) and cervical rigidity (69%). The incubation period varies from 3 days to 4 weeks. Most cases resulted from cut wounds (54%), to a lesser extent from excoriations (15%), and one case was associated with gynaecological surgery. The entry sites of the injuries were mainly on the upper (42%) and lower limbs (34.6%). Three patients had been vaccinated and 17 had not. Six cases were not recorded. The chief complications that developed were: dysautonomia (73%) and pneumonia (42%). The mortality rate was 69%. CONCLUSION: In spite of having suitable equipment available with which to treat tetanus, mortality is high, mainly because of dysautonomias. Prevention is therefore the most effective way of controlling this disease

Adolescent↗