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Biomedical subjects

H Slor

Publications and source records attributed to H Slor.

At least 73 records · Page 4Linked to original sources
PubMed1973

Purification of 14C-labelled deoxyribonuclease II from HeLa S3 lysosomes and its use as a marker for the study of nuclear deoxyribonuclease II.

Deoxyribonuclease II (DNAase II) in mammalian cells has generally been considered to be located in the lysosomes. Several recent studies have indicated that some DNAase II activity is present in purified nuclei; this, however, could have been due to some contamination of the nuclear fraction by lysosomes, or alternatively, it could have been caused by specific binding of lysosomal DNAase II to the nuclear fraction during isolation. Our previous studies have eliminated the possibility that lysosomal contamination was the cause of the presence of DNAase II in isolated nuclei. In this study I have purified (14)C-labelled lysosomal DNAase II and added it to cells during isolation of their nuclei. This study demonstrates that there is no specific binding of lysosomal DNAase II to the nuclear fraction and concludes that DNAase II activity observed in isolated nuclei represents an intrinsic activity that might be involved in nuclear DNA metabolism.

Binding Sites↗
PubMedDate not supplied

DNA repair in thymocytes of mice undergoing thymic involution.

DNA repair in mouse thymocytes before the onset of thymic involution was compared to that at successive stages during involution of the thymus. Repair of DNA in these cells after in vitro X-irradiation was evaluated by sedimentation of nucleoids in a sucrose gradient, as a measure of DNA supercoiling. DNA repair continued to function in the thymocytes even at ages when there was a dramatic reduction in the number of cells in the thymus.

Aging↗
PubMedDate not supplied

Xeroderma pigmentosum.

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Carcinoma, Squamous Cell↗
PubMedDate not supplied

Werner's syndrome (adult progeria): an affected mother and son presenting with resistant psychosis.

Age-related psychotic conditions may be studied by focusing on the unique group of progeroid syndromes. This report will focus on Werner's syndrome, one of the better defined and studied progeroid syndromes. We applied clinical and histophysiological evaluations to two patients, a mother and son, suffering from Werner's syndrome. Both patients presented with resistant psychosis and evidence of impaired cellular repair mechanisms. Psychiatric morbidity in Werner's syndrome is rarely reported. This syndrome can serve as a possible model for aging-associated development of psychosis.

Adult↗
PubMedDate not supplied

Improved reliability of a serum alpha-fetoprotein enzyme immunoassay compared with a radioimmunoassay.

During the 6-month period between September 1982 and February 1983, serum samples were obtained from 850 pregnant women attending the municipal family clinics in Tel Aviv. All serum samples were tested by alpha-fetoprotein (AFP) enzyme immunoassay (EIA), and 650 samples were also tested by AFP radioimmunoassay (RIA). Comparisons were made between the absolute values, the medians, and the multiples of the median (MOM) obtained by each method. The ratios of the 90th percentile to the median and of the median to the 10th percentile were also compared. Inter- and intraassay variation were determined for both methods. Correlating the test results with the birth results showed that both methods detected the four fetuses with a neural tube defect (NTD) and three of nine twin pairs, when using a cutoff point of greater than or equal to 2.5 MOM. Elevated maternal serum AFP (MSAFP) levels were also noted in 32 normal singletons (3.8%). There were no false negative results during this phase of the study. Although the median values were similar for both methods, the mean of the ratios of the median to the 10th percentile and the 90th percentile to the median, showed a reduced spread for the EIA method.

Evaluation Studies as Topic↗