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Biomedical subjects

H Singh

Publications and source records attributed to H Singh.

At least 649 records · Page 36Linked to original sources
PubMed1973

Myelin from human peripheral nerves. Quantitative and qualitative studies in two age groups.

Myelin in femoral nerve segments obtained at autopsy was isolated quantitatively by a series of discontinuous and continuous flotation procedures. The total amount of myelin isolated from these nerves was expressed as the sum of cholesterol, glycolipid, phospholipid, and protein and averaged 2.6+/-0.4 mg/g in a group aged 60-77 yr compared with 10.8+/-1.9 mg/g of nerve in a group aged 35-58 yr. The lower value in the older group remained apparent whether the myelin content was related to the whole nerve segment, its unit length or weight. This indicates that the decrease is an absolute one, not related to a change with aging in the nonmyelin content of nerve. No qualitative differences in myelin lipids were found between the two groups. Protein content was, however, significantly higher in the older group (34 and 28.7% of the total myelin weight, respectively). The decrease in myelin content with aging, observed by direct measurement in this study, may be the structural counterpart to age related alterations in peripheral nerve function-decreased conduction velocity, and impaired appreciation of vibration.

Adult↗
PubMed1973

Glycolipids of peripheral nerve: isolation and characterization of glycolipids from rabbit sciatic nerve.

Besides cerebreside and sulfatide four other glycolipids were isolated from rabbit sciatic nerve and analyzed by chemical and chromatographic methods. Three of the glycolipids were shown to be fatty acid esters of cerebroside; the fourth was characterized as diacyl glycerol galactoside and its alkyl ether analog. In the ester linkage mainly unsubstituted acids with chain length C(16) to C(18) were present. Both hydroxy and unsubstituted acids were found in amide linkage. They varied in chain length from C(16) to C(24) and were typical of cerebrosides. The long-chain base fraction contained sphingosine and dihydrosphingosine as the main components.

Animals↗