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Biomedical subjects

H Shiokawa

Publications and source records attributed to H Shiokawa.

69 records · Page 4Linked to original sources
PubMed1979

Complex formed from 3-hydroxy-4-formylpyridine, glutamic acid, and technetium--a possible cholescintigraphic agent.

In order to obtain a technetium-99m-labeled cholescintigraphic agent suitable for kit preparation, reactions of a variety of aromatic aldehydes, amino acids, and [99mTc] pertechnetate were examined under mild conditions. Aqueous solutions of the three reactants were heated in a boiling-water bath and the products analyzed by means of thin-layer chromatography for the formation of organic technetium complexes. 3-Hydroxy-4-formylpyridine (HFP) and 1-methyl-3-hydroxy-4-formylpyridinium chloride (N-Me-HFP) formed the complex with excellent yields, whereas 3-hydroxy-2-formylpyridine, 4-nitrosalicylaldehyde, salicylaldehyde, and isonicotinaldehyde did not. The complex is concluded to be the Tc-99m chelate of the Schiff base formed from the aldehyde and amino acbbits administered with the complex of HFP, glutamic acid and Tc-99m. The results indicate that it is promising as a cholescintigraphic agent.

Animals↗
PubMed1977

Fluorescent probes for antibody active sites. II. Further studies on two groups of anti-MANS antibodies with significantly different effects on MANSamide fluorescence produced by a single rabbit.

As described previously, a single rabbit (No. 13) produced two groups of anti-MANS antibodies with significantly different effects on MANSamide fluorescence. These groups, anti-MANS antibody (415 nm) and anti-MANS antibody (465 nm), had emission maxima at 415 nm and 465 nm, respectively. This paper deals with the immunochemical properties of the two groups of antibodies (1) The results of fluorometric titration of these groups with MANSamide indicated that anti-MANS antibody (465 nm) had a higher affinity for MANSamide than did anti-MANS antibody (415 nm). (2) More than 70% of anti-MANS antibody (415 nm) was adsorbed on Sepharose covalently coupled with bovine serum albumin, which was used as a carrier during immunization, while a hlaf of anti-MANS antibody (465 nm) was adsorbed. (3) Only one of the groups, anti-MANS antibody (465 nm), had precipitating activity toward MANS-rabbit serum albumin used as a test antigen.

Anilino Naphthalenesulfonates↗
PubMed1976

Fluorescent probes for antibody active sites. I. Production of antibodies specific to the N-methyl-2-anilinonaphthalene-6-sulfonyl group in rabbits and some fluorescent properties of the hapten bound to the antibodies.

1. Rabbits were immunized with N-methyl-2-anilinonaphthalene-6-sulfonyl (MANS) bovine serum albumin (MANS-BSA) prepared by the reaction of bovine serum albumin with N-methyl-2-anilinonaphthalene-6-sulfonyl chloride to obtain IgG fraction containing anti-MANS antibodies. 2. Both N-methyl-2-anilinonaphthalene-6-sulfonate (MANSate) and N-methyl-2-anilinonaphthalene-6-sulfonamide (MANSamide), which are virtually non-fluorescent in aqueous solution, became strongly fluorescent, with a blue shift of about 100 nm, when they were specifically bound to the antibodies against the hapten group contained in the IgG fractions. 3. IgG fractions were obtained from five immunized rabbits at intervals after the primary immunization. The fluorescence characteristics of the hepten were carefully examined with preparations of IgG fraction differing in source and time of bleeding. One of the rabbits showed a very interesting immune response. The rabbit produced two groups of anti-MANS antibodies which were significantly different in their effect on the fluorescence properties of the hapten group. One of the groups was exclusively produced at the early stage in the immune response. The other group appeared at the late stage. It is suggested that the two groups differ as regards the structure of the hepten combining sites. 4. The above findings indicate that the MANS group can be successfully used as a hapten with useful properties as a fluorescent probe.

Anilino Naphthalenesulfonates↗