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Biomedical subjects

H Shinmoto

Publications and source records attributed to H Shinmoto.

At least 37 records · Page 2Linked to original sources
PubMed1992

Transformation of human colostrum lymphocytes with Epstein-Barr virus.

Lymphocytes from human colostrum were transformed with EB virus to obtain immunoglobulin secreting cells. Equal proportions of IgG, IgA and IgM were detected in wells containing transformed colostrum cells. Pretreatment of colostrum cells with BCGF prior to EB virus infection reduced the transformation slightly. We discussed the possibility of establishing cell lines that produced human monoclonal antibody using colostrum cells.

Cell Transformation, Viral↗
PubMed1991

[Fallopian tube recanalization with a catheter system--experience in the use of a balloon catheter].

Fallopian tube obstruction is one of the most difficult problems in the treatment of infertility. This report gives the results of a pilot study on the transcervical recanalization of the occlusive fallopian tube. Selective catheterization of the uterine cornu was applied through a balloon catheter, which was wedged at the internal uterine os. In 16 occlusive fallopian tubes of 11 cases, the catheterization procedure was attempted and accomplished with a 87.5% success rate. Recanalization was successful in 75.0% of the affected tubes. Subsequent pregnancy was confirmed in three cases. This convenient technique is safe and effective and it will be accepted as the first choice in the diagnosis and treatment of fallopian tube obstruction.

Adult↗
PubMed1991

Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies.

Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (kappa) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (lambda) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both kappa and lambda light chains were obtained. Two of them secreted antibodies with antiricin specificity (kappa and lambda light chains) and antidiphtheria toxin specificity (kappa and lambda light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain kappa light chain, but lambda chain. A hybrid hybridoma clone 5-29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.

Animals↗
PubMed1990

Purification and some properties of proliferation suppressing factor from human lung adenocarcinoma PC-8.

Extracts by 3 M KCl from a human lung adenocarcinoma cell line, PC-8, suppressed the proliferation of phytohemagglutinin activated lymphocytes. The proliferation suppressing factor (PSF) was purified from the extracts by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The purified PSF had a molecular weight of 50 kDa by SDS-PAGE and was acid- and heat-labile. Since the PSF also suppressed the proliferation of three established human cell lines, it is unlikely that the PSF hinders the interaction between lymphocytes and mitogens.

Adenocarcinoma↗
PubMed1990

Stabilization of tumor antigens by chemical modification with diethyl pyrocarbonate.

C3H mice were immunized with MH134 tumors modified with two different chemicals, DEPC and TNBS, and the tumor-neutralizing activities of the spleen cells were examined after separating the cells by a Nylon-wool column, plastic dish, or treatment with specific antibodies (anti-mouse IgG and anti-mouse theta sera). The effector cells induced in mice by immunization with TNBS-treated tumors were T cells. In contrast, in the case of tumors modified with DEPC, macrophages were the major effector cells. The effects of chemical modification on the solubilization of cell surface proteins was also examined. DEPC inhibited solubilization of specific proteins from tumor cell surfaces, while TNBS did not have such an effect on the solubilization of proteins. These results indicate that the antitumor response in mice primed with DEPC-modified tumor is different from the case of TNBS-modified tumors, and that the immunological significance of chemical modification with DEPC is supposedly due to the stabilization of antigenic proteins on the tumor cell surface.

Animals↗
PubMed1989

[Effect of various contrast media on platelet aggregation--an in vivo study].

Recently, some radiologists using non-ionic contrast media for angiography have noted the increased tendency of thrombus formation in the injection syringe or angiographic catheter contaminated with blood. In vitro studies by some authors have shown that non-ionic contrast media had only a slight anticoagulative effect as compared with ionic contrast media. But the in vivo studies comparing the anticoagulative effect of both ionic and non-ionic contrast media have not been performed previously. We investigated the effect of non-ionic and ionic contrast media on platelet aggregation in 40 patients undergoing angiography. The in vivo study revealed a negligible influence of both non-ionic and ionic contrast media on systemic platelet aggregation. The dose of contrast media also showed no significant correlation with platelet aggregation. Our study suggests that the data from in vitro experiments cannot be extended to in vivo study as for the relationship between contrast media and their anticoagulant effect.

Adult↗
PubMed1988

Human hybrid immunoglobulin M containing immunoglobulin A.

Some hybridoma clones made by fusion of a human lymphoblastoid cell line, HO323 with human B lymphocytes, secreted not only IgA but also IgM-like immunoglobulin molecules. The IgM-like immunoglobulin had a molecular size of 900 K which corresponded to that of IgM. Immunochemical analyses revealed that the IgM-like immunoglobulin contained two monomeric IgA and three monomeric IgM molecules. In the IgA moieties, half of original light chains were replaced with kappa chains derived from the IgM, and vice versa.

Cell Line↗
PubMed1987

One-step isolation of lactoferrin using immobilized monoclonal antibodies.

Immunoaffinity columns made with monoclonal antibodies to either human or bovine lactoferrins were prepared to isolate human lactoferrin or bovine lactoferrin from milks by a single chromatographic step. Recoveries of human lactoferrin and bovine lactoferrin were 98 and 97%, respectively. The human lactoferrin recovered from defatted human colostrum was 98% pure with 93% iron-binding capacity. Amount of recovered bovine lactoferrin, as well as purity and iron-binding capacity, varied widely depending on the source of bovine milks and pretreatments (particularly pasteurization temperature). The best source to isolate bovine lactoferrin was raw skim milk yielding a protein 97% pure and with a 99% iron-binding capacity. Thus, immunoaffinity chromatography provides an effective one-pass isolation of highly pure human or bovine lactoferrin with reasonable recovery and iron-binding capacity.

Animals↗
PubMed1986

IgA specific helper factor (alpha HF) in human colostrum.

Induction of immunoglobulin secretion by human colostrum was investigated using human peripheral blood lymphocytes (PBL) and Epstein-Barr virus transformed human B lymphoblastoid cells. Stimulation of the cells with colostrum induced IgA plaque forming cells but neither IgG nor IgM plaque forming cells, indicating the occurrence of IgA specific helper factor (alpha HF) in human colostrum. alpha HF activity was eluted into fractions with an apparent molecular weight of about 80 kD by gel filtration, and with a PI range of 5.8 to 6.2 by chromatofocusing. IgA secreted by PBL stimulated with alpha HF had a similar molecular weight distribution to that of IgA in human colostrum. From these results a hypothesis is proposed; IgA-committed B cells in the mammary gland differentiate to plasma cells producing dimeric IgA after stimulation by alpha HF so that the dominant immunoglobulin in human colostrum is IgA.

Antibody-Producing Cells↗
PubMed1983

Physicochemical and immunochemical properties of a thermo-labile antigen (TLA a) from yeast cell surface.

The physicochemical and immunochemical properties of a thermo-labile antigen (TLA a) which is located on the cell surface of Saccharomyces cerevisiae were studied. The sedimentation constant (S20,W) and molecular weight (sedimentation equilibrium method) were 6.26S and 68,800, respectively. The circular dichroic (CD) spectrum of TLA a had negative maxima at 210 and 221 nm, indicating the presence of alpha-structure of a polypeptide chain. The molar ratio of antibody to antigen which gave maximum precipitation was 2.7. Approximately 50% of the antigenic activity of heat-denatured TLA a was recovered when denatured molecules were dissolved in 6 M guanidine hydrochloride followed by 25-fold dilution with H2O. The amount of TLA a existing on the yeast cell surface was estimated to be 37.5 micrograms per 10.5 mg of fresh cells, corresponding to 0.36% by weight of the fresh yeast.

Antigens, Fungal↗
PubMedDate not supplied

Small renal cell carcinoma: MRI with pathologic correlation.

The MRI features of small renal cell carcinomas (RCCs) were retrospectively reviewed and correlated with histology in 24 patients. MRI features on both T1- and T2-weighted images were classified into hypointensity, isointensity, and hyperintensity. Each tumor was pathologically classified into four types: alveolar, papillary, tubular, and cystic. These findings were correlated with MR signal intensities. Alveolar tumors showed hypointensity to isointensity on T1-weighted image and isointensity to hyperintensity on T2-weighted image. In contrast, all papillary tumors showed hypointensity on T2-weighted image. Four of six tumors with hypointensity on T2-weighted image were caused by hemosiderin deposition, hemorrhage, and necrosis. However, there were two papillary RCCs that showed hypointensity on T2-weighted image despite no hemosiderin deposition and no hemorrhage. We conclude that papillary RCC is associated with T2-hypointense appearance as well as hemosiderin deposition, hemorrhage, and necrosis.

Adult↗
PubMedDate not supplied

Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages.

A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of Fc receptor, phagocytic activities in U937. Furthermore, U937 cells, which were activated with STL-1 exhibited cytotoxic activity against human lung carcinoma cell line A549. However, STL-1 did not affect growth of a normal human fetal lung cell line TIG-1. The individual components of STL-1, neither sugar moiety nor fatty acids in the free form, were effective at inducing the differentiation of U937 cell. From these results, we concluded that STL-1 has low cytotoxicity against normal human cells and the ester molecule itself is responsible for the activity of inducing differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophage which results in the stimulation of the production of some cytotoxic substances.

Biomarkers↗
PubMedDate not supplied

MRI of fetal abdominal abnormalities.

Although ultrasonography is the method of choice for evaluating the fetus, magnetic resonance imaging (MRI) complements ultrasonography in the accurate diagnosis of fetal abnormalities. The advantages of MRI include excellent tissue contrast, a large field of view, and relative operator independence. To date, most studies on fetal MRI have focused on the fetal central nervous system and thoracic disorders. However, our experience suggests that MRI can be helpful even in evaluating fetal abdominal disorders. This pictorial essay illustrates the various MRI appearances of fetal abdominal abnormalities and discusses the indications and advantages of fetal MRI.

Abdominal Wall↗