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Biomedical subjects

H Shiina

Publications and source records attributed to H Shiina.

At least 73 records · Page 4Linked to original sources
PubMed1993

Interaction of testosterone-oestradiol binding globulin (TeBG) and albumin with human prostatic carcinoma in vitro.

During a period of incubation, the accumulation of testosterone-oestradiol binding globulin (TeBG) and albumin were noted in the cells of human prostatic carcinoma in vitro. These accumulations were inhibited by addition of rabbit anti-human TeBG antibody. However, the accumulation of human chorionic gonadotropin was not prominent. These observations suggest that TeBG and albumin are incorporated into the cells of human prostatic carcinoma.

Antibodies, Neoplasm↗
PubMed1992

Organisms in the prostate and antibiotics in the treatment of postoperative infections.

On 24 benign prostatic hyperplasia patients with preoperatively sterile urine, who also had no history of urinary tract infection, the organisms in the prostate obtained through transurethral resection as well as in the anterior urethra were isolated. In 17 patients out of 24, organisms obtained in the prostate were identical to those in the anterior urethra; therefore, it can be concluded that another 7 patients had organisms in the prostate itself before the operation. These results would suggest that sterile urine did not indicate sterile prostate and that organisms in the prostate did not always ascend from the urethra. In the patients who preoperatively received transurethral catheterizations, Streptococcus faecalis was the most predominantly isolated organism in the prostate, which was highly sensitive to ABPC and minocycline and lower to cephems. On the other hand, in the patients with no history of catheterizations, Staphylococcus epidermidis was the most commonly isolated one, which was highly sensitive to ABPC and minocycline as well as cephems. So, in the treatment of the cases with infectious symptoms after transurethral resection of the prostate, ABPC or minocycline should be selected as first-choice drugs rather than cephems.

Aged↗
PubMed1991

Unstable chromosome aberrations in bladder and renal cell carcinoma.

Structural chromosome aberrations were investigated in 18 bladder carcinoma and 14 renal cell carcinoma patients. Unstable chromosome aberrations, such as breaks, gaps, minutes, and fragments, appeared in varying proportion. Double minutes (dmin) were also observed. In bladder carcinoma, 10 kinds of aberration were observed in 38.8% of the metaphase analyzed, and, in renal cell carcinoma, 7 kinds of aberrations were observed in 15.0%. The frequency of chromosome aberrations observed, and the occurrence of dmin and of aneuploidy, were correlated positively with histopathological grade and stage. From the results obtained here, we conclude that study of chromosome aberrations should be added as one of investigations for evaluating the malignant potential of the tumors.

Aged↗
PubMed1989

Study of estramustine binding protein (EMBP): purification of rat EMBP and establishment of RIA.

Estramustine binding protein (EMBP) was purified from the ventral prostate of the rat using DEAE-cellulose chromatography, concanavalin-A affinity chromatography and DEAE-sepharose chromatography. At the final step of the purification, two different peaks (Peaks A and B) of A280 nm were obtained. Peak A had a high binding activity to [3H] estramustine. On the other hand, Peak B had a low binding activity. On the analysis of polyacrylamide gel electrophoresis, Peak A gave two protein bands, whereas Peak B gave a single band. The molecular weight of the markedly stained band of Peak A was approximately 27,000, whereas that of Peak B was 18,000, as estimated by analysis of Fargusson's plot. The antibody against Peak B was used for establishing a radioimmunoassay (RIA) of EMBP. The sensitivity of this assay system was sufficient to measure of 1 ng of EMBP. The dilution curve of rat prostatic cytosol was paralleled with the standard curve. As a result obtained from this RIA, the mean concentration of immunoreactive EMBP was 8.01 ng/mg cytosol protein in human benign hyperplastic prostate (BPH) and 4.28 ng/mg protein in human prostatic carcinoma (PC), respectively. These results here obtained indicate that human prostate has an immunoreactive protein to the purified EMBP obtained from the ventral lobe of rat prostate.

Animals↗
PubMed1989

Urological complications in gynaecological diseases. Review of 18 cases requiring diversion or reconstruction of the urinary tract.

In 18 gynaecological patients requiring diversion or reconstruction of the urinary tract, the relationship between the urological treatments and their influences on the renal function based on DIP were studied. Double-J catheterization and percutaneous nephrostomy drainage with Fr 8 catheter are easily obstructed due to infection of the urinary tract, so a thicker catheter should be used to maintain drainage. Ureterocutaneostomy is considered to be a suitable method to divert urine in cases of poor general condition and poor prognosis in order to preserve renal function for a longer time.

Female↗
PubMed1980

Glycine stimulated growth hormone release in man.

The influence of glycine, the simplest amino acid, on pituitary function has been investigated in the present study. Different doses (4, 8, or 12 g) of glycine were intravenously infused over 15 or 30 min in normal subjects. Serum levels of GH (growth hormone) and Prl (prolactin) were measured before and after the infusion, and also blood sugar levels were determined. The dose of 4 or 8 g glycine induced a significant increase in serum GH (P less than 0.05 or P less than 0.001, respectively); however, a more pronounced and significant increase in serum GH levels was observed after infusion at a dose of 12 g glycine (P less than 0.001). It was clearly observed that the dose-dependent GH release to intravenous glycine occurred in normal subjects. On the contrary, serum Prl level was not changed significantly, and blood sugar level was transiently, but significantly (P less than 0.05), increased after the infusion of 12 g glycine. The present data suggest that glycine might play an important role in the control of hypothalamic-pituitary function.

Adolescent↗