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Biomedical subjects

H Shi

Publications and source records attributed to H Shi.

224 records · Page 13Linked to original sources
PubMed1990

[Anti-inflammatory effect of aconitines].

Aconitines are the total alkaloids isolated from the main root of Aconitum carmichaeli. They have marked suppressive effect on the swelling of rat's hind paw induced by injections of fresh egg protein, carrageenin, histamine and 5-HT, on the mouse's ear oedema induced by bimethylphenyl, on the exudate and proliferation of granulation tissue of granuloma pouch induced by croton oil, on the leukocyte migration and on the contents of PGE in the exudate of the swelling hind paw induced by injecting carrageenin. Aconitines have been found useful in inhibiting significantly such immune inflammations as reversible passive Arthus reaction, rat's delayed skin hypersensitivity and adjuvant arthritis. They have also a noticeable analgesic effect.

Aconitine↗
PubMed1988

Effects of activating spinal alpha-adrenoreceptors on sympathetic nerve activity in the rat.

The effects of several alpha-adrenoreceptor agonists and antagonists administered intrathecally at T10 level on renal sympathetic nerve activity (RSNA) were examined, in chloralose-urethane anaesthetised rats. Intrathecal noradrenaline (NA, 0.5-500 micrograms) produced one of 3 responses depending on dose, an inhibition of RSNA at low doses, an excitation of RSNA at high doses, or a biphasic effect. Intrathecal adrenaline (5-200 micrograms) was inhibitory in the main but some doses elicited poorly repeatable brief excitation followed by prolonged inhibition. Intrathecal methoxamine (ME; 2.5 ng-25 micrograms) caused a dose-dependent increase in RSNA (mean maximum response 27 +/- 0.5%). The excitatory effects of NA and ME were blocked (72% +/- 12%) by pretreatment with intrathecal prazosin (PRA, 20-200 ng) but not by yohimbine (YOH, 200 ng). Intrathecal guanabenz (GUA 3-15 micrograms) caused a dose-dependent inhibitory effect on RSNA (mean maximum 32% +/- 5%). The inhibitory effects of NA, adrenaline and GUA were blocked by pretreatment with intrathecal YOH (200 ng-2 micrograms). Intrathecal PRA (200 ng) had no effect on the inhibitory effects of NA and GUA. Intravenous administration of each of the adrenoreceptor agonists (apart from adrenaline), at similar doses to those given intrathecally, in most cases had no significant effect on RSNA; in a few cases the opposite effects to those produced by intrathecal administration were seen.

Adrenergic alpha-Agonists↗
PubMedDate not supplied

On-line metabolic pathway analysis based on metabolic signal flow diagram.

In this work, an integrated modeling approach based on a metabolic signal flow diagram and cellular energetics was used to model the metabolic pathway analysis for the cultivation of yeast on glucose. This approach enables us to make a clear analysis of the flow direction of the carbon fluxes in the metabolic pathways as well as of the degree of activation of a particular pathway for the synthesis of biomaterials for cell growth. The analyses demonstrate that the main metabolic pathways of Saccharomyces cerevisiae change significantly during batch culture. Carbon flow direction is toward glycolysis to satisfy the increase of requirement for precursors and energy. The enzymatic activation of TCA cycle seems to always be at normal level, which may result in the overflow of ethanol due to its limited capacity. The advantage of this approach is that it adopts both virtues of the metabolic signal flow diagram and the simple network analysis method, focusing on the investigation of the flow directions of carbon fluxes and the degree of activation of a particular pathway or reaction loop. All of the variables used in the model equations were determined on-line; the information obtained from the calculated metabolic coefficients may result in a better understanding of cell physiology and help to evaluate the state of the cell culture process.

Models, Biological↗
PubMedDate not supplied

The comparative effects of lansoprazole, omeprazole, and ranitidine in suppressing gastric acid secretion.

The effects on 24-hour intragastric pH levels of once-daily doses of lansoprazole 15 mg and lansoprazole 30 mg were compared with the effects of omeprazole 20 mg QD and ranitidine 150 mg QID in a phase I, randomized, double-masked, four-way crossover study conducted in 29 healthy male volunteers. Subjects received each treatment regimen for 5 consecutive days with at least a 2-week washout between treatment periods. Ambulatory 24-hour intragastric pH values were monitored in each subject at baseline (2 days before crossover period 1) and again before dosing on day 5 of each of the four crossover treatment periods. Gastric pH values increased during all four regimens, with significantly higher mean 24-hour pH values noted in subjects receiving lansoprazole 30 mg QD (4.53 +/- 0.16) compared with those receiving lansoprazole 15 mg QD (3.97 +/- 0.16), omeprazole 20 mg QD (4.02 +/- 0.16), or ranitidine 150 mg QID (3.59 +/- 0.16). Lansoprazole 30 mg produced significantly greater mean percentages of time that the gastric pH was above 3.0 and 4.0 (75% and 63%, respectively) compared with the other treatment regimens. The mean percentages of time during which gastric pH was above 3.0 and 4.0, respectively, for the other treatments were lansoprazole 15 mg, 64% and 48%; omeprazole 20 mg, 63% and 51%; and ranitidine 150 mg, 52% and 38%. All treatment regimens were well tolerated, with no clinically significant differences between the regimens. Multiple-dose lansoprazole 30 mg QD produced a significantly increased intragastric pH level and significantly longer durations of increased intragastric pH level compared with lansoprazole 15 mg QD, omeprazole 20 mg QD, and ranitidine 150 mg QID.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMedDate not supplied

Characterization of Bovidae sex-determining gene SRY.

In mammals, testis determination is under the control of the sex-determining gene SRY. This Y-linked gene encodes a protein with a DNA binding domain similar to those found in high-mobility-group proteins. Here we report the cloning and sequences of the SRY genes of yak and Chinese native cattle. Our data show that SRY genes in Bovidae are less divergent, especially in the coding and 3' regions.

3' Untranslated Regions↗