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Biomedical subjects

H Seki

Publications and source records attributed to H Seki.

At least 55 records · Page 3Linked to original sources
PubMed2000

Gram stain index of a natural bacterial community at a nutrient gradient in the freshwater environment.

Species succession of a natural bacterial community was indicated by the Gram stain index (GSI) using an experimental nutrient gradient in an oligotrophic pond created with an in situ gradostat. Various trophic conditions, ranging from eutrophic to mesotrophic, were formed experimentally with continuous inflow of sterile peptone solution in the gradostat, while the water mass outside the gradostat was oligotrophic. The GSI spectra of the natural bacterial populations show different profiles in accordance with the trophic level of the ambient water and the time progression of adaptation to different levels of eutrophication. Therefore, GSI can be used as a potential indicator of organic pollution in aquatic environments.

Journal Article↗
PubMed2000

Tubular injury as a cardinal pathologic feature in human heme oxygenase-1 deficiency.

Heme oxygenase (HO) catalyzes degradation of heme to biliverdin, iron, and carbon monoxide. It consists of three isoforms: an inducible form (HO-1), a constitutive form (HO-2), and the third isoform (HO-3), with properties similar to HO-2. There is limited evidence to suggest that the induction of HO-1 may have anti-inflammatory effects in an in vivo model of oxidative stress-mediated renal injury. We experienced the first human case of HO-1 deficiency. The patient had persistent proteinuria and hematuria, with biochemical evidence of renal tubular injury. We obtained three consecutive renal specimens: two from renal biopsies at 2 and 5 years of age and the third from autopsy at 6 years of age. The patient had systemic vascular endothelial-cell injury with massive intravascular hemolysis. The serum was loaded with heme and a large amount of heme-conjugated haptoglobin. A high concentration of haptoglobin was also detectable in urine. Mesangial proliferation or change in glomerular capillary-wall thickness was relatively mild to moderate in all specimens. Electron microscopic examination showed widespread endothelial detachment and subendothelial deposits of an unidentifiable material. It was striking that tubulointerstitial injury, with tubular dilatation and/or atrophy, interstitial fibrosis, and inflammatory cell infiltration, advanced progressively. Tubular epithelial cells were injured, and massive deposition of iron and haptoglobin was detectable. Bowman's capsules were dilated significantly, probably secondary to the collapse of atrophic tubuli. This is the first report to show that HO-1 has critical roles in vivo in protecting renal tubuli, in addition to vascular endothelium, from oxidative injury.

Child, Preschool↗
PubMed2000

The effects of the traditional chinese medicine, "Banxia Houpo Tang (Hange-Koboku To)" on the swallowing reflex in Parkinson's disease.

Swallowing disorder is common in Parkinson's disease (PD). We studied the swallowing disorder in PD, and tested the efficacy of Banxia Houpo Tang (BHT, a Chinese traditional medicine) in improving the swallowing reflex of PD patients. The Swallowing reflex test is a simple method used to detect swallowing disorders in patients with cerebrovascular disease. Because we observed previously that BHT significantly improved the swallowing reflex in cerebrovascular patients, we studied whether BHT was also effective in improving the swallowing disorder in patients with PD. 23 PD patients (13 males, 10 females, mean age 66.0+/-9.3, Hoehn & Yahr (H-Y) mean score = 2.8) were evaluated for swallowing reflex and the concentration of substance-P in their saliva before and after 4 weeks of BHT treatment. The swallowing reflex before treatment was significantly delayed, according to the H-Y score (Spearman's p = 0.014, R2 = 0.463). The swallowing reflex before BHT treatment was 3.66+/-0.98 sec, and after BHT treatment, it improved significantly, to 2.27+/-0.54 sec (p < 0.0001). Substance-P concentration in PD patients saliva before treatment was significantly lower than in healthy controls (p = 0.007), but showed no significant change after BHT treatment. Our research shows that the swallowing reflex is an effective method to evaluate the swallowing disorder in PD. BHT can significantly improved the swallowing reflex in PD patients, and therefore can be a hopeful candidate for preventing aspiration pneumonia in PD.

Aged↗
PubMed2000

Identification of deoxymiroestrol as the actual rejuvenating principle of "Kwao Keur", Pueraria mirifica. The known miroestrol may be an artifact.

Miroestrol (1) has been isolated previously as an active principle from "Kwao Keur" (Pueraria mirifica), a rejuvenating folk medicine from Thailand. Reinvestigation using bioassay-guided purification has resulted in the isolation of a new potent phytoestrogen, deoxymiroestrol (2). The facile aerial oxidation of 2 into 1 suggests the possibility that 1 may be an artifact.

Antineoplastic Agents, Phytogenic↗
PubMed2000

Characterization of immune function and analysis of RAG gene mutations in Omenn syndrome and related disorders.

Omenn syndrome was recently found to be caused by missense mutations in RAG1 or RAG2 gene that result in partial V(D)J recombination activity. Although the clinical hallmarks of the disease are well defined, there have been several cases with clinical findings similar to, but distinct from Omenn syndrome. The data on immune functions and RAG gene mutations of such cases are limited. We described five Japanese infants from four unrelated families, including two cases of Omenn syndrome and three cases of related disorders. Sibling cases with typical Omenn phenotype were found to be compound heterozygotes of R396C and L885R mutations in RAG1. The former has been reported in European cases and may constitute a hot spot. The latter is a novel missense mutation. Infants with related disorders exhibited erythroderma, eosinophilia, hypogammaglobulinaemia, decreased number of B cells and skewing to Th2, and their lymph node specimens showed architectural effacement, lymphocyte depletion and histiocytic hyperplasia, each of which is seen characteristically in Omenn syndrome. However, in these cases serum IgE levels were low or undetectable. We found no mutation in RAG genes except for a K820R substitution in RAG1, which was regarded to be a functional polymorphism, in two of these cases. Our study suggests that RAG missense mutation may be a genetic abnormality unique to Omenn syndrome with characteristic clinical and laboratory findings. Variations of Omenn syndrome, or related disorders, may represent a different type of immunodeficiency, distinct from abnormalities in lymphoid-specific recombinase activity.

Apoptosis↗
PubMed2000

Diminished expression of CD59 on activated CD8+ T cells undergoing apoptosis in systemic lupus erythematosus and Sjögren's syndrome.

The present study was undertaken to examine the phenotype of T cells undergoing in vitro apoptosis in patients with autoimmune diseases such as systemic lupus erythematosus (SLE) and primary Sjögren's syndrome (SS). Compared with normal controls, we found diminished expression of CD59 antigen (one of the cell-surface complement-regulatory proteins) on CD8+ T cells, but not on CD4+ T cells, from patients with SLE and SS. Three-colour immunofluorescence analysis revealed that these CD59dim CD8+ T cells were activated T cells, expressing both human leucocyte antigen (HLA)-DR and CD45RO antigens. In addition, these CD59dim CD8+ T cells were more susceptible to in vitro apoptosis than CD59bright CD8+ T cells. In two patients with active lupus, the percentage of CD59dim CD8+ T cells was significantly decreased after steroid therapy. These findings suggest that decreased expression of CD59 antigen on in vivo-activated CD8+ T cells may be correlated with disease activity and may be involved in activation-induced apoptosis in patients with SLE and SS.

Apoptosis↗
PubMed2000

FDG PET in the evaluation of the aggressiveness of pulmonary adenocarcinoma: correlation with histopathological features.

2-[Fluorine-18]fluoro-2-deoxy-d-glucose (FDG) uptake within the primary lesion correlates with survival on positron emission tomography (PET) studies of patients with non-small cell lung cancer. The more metabolically active the tumour, the worse the outcome. The aim of this study was to determine whether a correlation exists between aggressiveness as determined by pathology and the findings of FDG PET in pulmonary adenocarcinoma. Thirty-five patients with 38 adenocarcinomas of the lung were studied. All patients underwent thoracotomy within 4 weeks of the FDG PET study. For semiquantitative analysis, standardized uptake values (SUVs) were calculated. Patients were classified into high SUV (> or = 4.0) and low SUV (<4.0) groups. The degree of FDG uptake (SUVs) in primary lung lesions was correlated with the histopathological features of aggressiveness (pleural involvement, vascular invasion or lymphatic permeation). The mean SUV of aggressive adenocarcinomas (4.36+/-1.94, n = 22) was higher than that of non-aggressive ones (1.53+/-0.88, n = 16) (P < 0.0001). Tumours with a high FDG uptake have a significantly higher likelihood of aggressiveness than those with a low FDG uptake (P = 0.0004). Analysis by the Kaplan-Meier methods revealed that the groups had different prognoses (log-rank test, P = 0.0099). The high SUV group had a significantly worse prognosis. In conclusion, a correlation was seen between aggressiveness as determined by pathology and glucose metabolism as measured by FDG PET in adenocarcinoma of the lung. FDG PET may be used as a non-invasive diagnostic technique in measuring aggressiveness and prognosis in patients with pulmonary adenocarcinoma.

Adenocarcinoma↗
PubMed2000

Value of dynamic MR imaging in assessing endometrial carcinoma involvement of the cervix.

OBJECTIVE: The purpose of this study was to evaluate endometrial carcinoma involvement of the cervix using dynamic MR imaging compared with T2-weighted and contrast-enhanced T1-weighted MR imaging. SUBJECTS AND METHODS: In 42 patients with endometrial carcinoma, T2-weighted MR imaging using rapid acquisition with relaxation enhancement, dynamic MR imaging using gradient-echo sequences, and contrast-enhanced T1-weighted MR imaging using spin-echo sequences were performed before treatment. We evaluated patterns of enhancement in the cervix and tumor. In 39 of the 42 patients who underwent surgical treatment, we compared MR imaging findings with histologic results concerning cervical involvement. RESULTS: Enhancement of the cervical epithelium was greater than that of the tumor and cervical stroma on dynamic MR imaging in most patients. In assessing cervical involvement, the accuracy of T2-weighted, dynamic, and contrast-enhanced T1-weighted MR imaging was 85%, 95%, and 90%, respectively; no statistically significant difference was observed. False-positive cases on T2-weighted MR imaging were correctly identified as having no cervical involvement on dynamic MR imaging using the finding of continuous enhancement of the cervical epithelium. We found this finding to be reliable in assessing tumor involvement of the cervix. CONCLUSION: We believe that, in combination with T2-weighted MR imaging sequences, dynamic MR imaging is useful in assessing endometrial carcinoma involvement of the cervix.

Adult↗
PubMed2000

FDG PET measurement of the proliferative potential of non-small cell lung cancer.

UNLABELLED: The goals of this study were to correlate FDG uptake with cell proliferation and cellular density in non-small cell lung cancer. METHODS: Thirty-one patients with 32 non-small cell lung cancers were examined with FDG PET. For semiquantitative analysis, standardized uptake values (SUVs) were calculated. All patients underwent thoracotomy within 4 wk after the FDG PET study. Cell proliferation was immunohistochemically assessed as the relative number of cells expressing the proliferating cell nuclear antigen ([PCNA] labeling index). Cellular density was also evaluated using light microscopy. RESULTS: SUVs correlated significantly with PCNA labeling index (r = 0.740; P < 0.0001) but only weakly with cellular density (r = 0.392; P = 0.0266). High FDG uptake correlated with high PCNA expression. The PCNA labeling index and SUVs were significantly lower in bronchioloalveolar carcinomas (n = 8) (12.3 +/- 9.45% and 1.45 +/- 0.76, respectively) than in nonbronchioloalveolar carcinomas (n = 19) (33.5 +/- 21.8%, P = 0.015, and 3.75 +/- 1.93, P = 0.003, respectively). However, no significant differences in cellular density were seen between bronchioloalveolar carcinomas and nonbronchioloalveolar carcinomas. CONCLUSION: FDG uptake is related to cell proliferation rather than to the cellular density of non-small cell lung cancer.

Carcinoma, Non-Small-Cell Lung↗
PubMed2000

[Significance of cancer cell growth and apoptosis in morphogenesis of colorectal cancer].

The present study was carried out to elucidate the relationship between cancer cell growth and apoptosis in morphogenesis of colorectal cancer, and the role of p53 expression, one of apoptosis-related genes, in apoptosis. Specimens from 82 cases of colorectal cancer, all surgically resected, were divided into intramucosal polypoid growth (PG) and nonpolypoid growth (NPG) types according to Shimoda's classification. Expressions of proliferating cell nuclear antigen (PCNA) and p53 protein were assessed by immunohistochemical staining, and apoptosis by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. Immunohistological detection of p53 protein was performed by the use of Pab1801. In the assessment of PCNA and apoptosis the numbers of positive cells per 1000 tumor cells were expressed as PCNA labeling index (L.I.) and apoptosis labeling index (Apo L.I.), respectively. In tumor invading muscularis propria (mp), the cancer tumor tissue was equally divided into three by the depth, top, middle, and bottom, and the number of apoptosis-positive cells per 500 tumor cells was calculated per each depth of invasion and compared between PG and NPG types. PCNA L.I. was 658.5 +/- 127.1 (mean +/- SD) in PG type. When determined according to depth of invasion, PCNA L.I. was 533.8 +/- 188.2 for tumor invading submucosa (sm), 741.8 +/- 62.2 for mp, and 679.2 +/- 94.3 for tumor invading subserosa or penetrating the serosa (ss-a). The corresponding values in the NPG type were 651.9 +/- 176.2, 482.2 +/- 227.1, 766.2 +/- 90.7, and 690.9 +/- 84.3, respectively. There was no significant difference in the relationship of PCNA L.I. with the depth of invasion in both growth types. Apo L.I. was 19.9 +/- 9.8 in PG type. When determined according to depth of invasion, Apo L.I. was 29.2 +/- 9.7 for sm, 21.7 +/- 9.0 for mp, and 15.4 +/- 7.0 for ss-a. The corresponding values in the NPG type were 52.8 +/- 25.1, 67.5 +/- 25.6, 37.2 +/- 9.4, and 52.6 +/- 26.3, respectively. Apo L.I. of NPG type was significantly higher than that of PG type in each depth of invasion (p < 0.01). In mp tumor the numbers of apoptosis-positive cells in the top, middle, and bottom of the PG type were 8.0 +/- 4.8, 11.8 +/- 3.9, and 12.9 +/- 5.9, and the corresponding values of the NPG type were 26.6 +/- 7.6, 17.3 +/- 5.3, and 11.8 +/- 4.3, respectively. The number of apoptosis in the top and middle of the lesion of NPG tumors was significantly higher than that of PG tumors. There was no difference in the number of apoptosis in the bottom of the lesion between the NPG and the PG types. There was no significant difference in the Apo L.I. between p53 positive cancers and negative cancers. These results indicate that colorectal cancers show a pattern of PG if apoptosis is low, and a pattern of NPG if apoptosis is high, and that apoptosis of colorectal cancer cell is not regulated by p53.

Apoptosis↗
PubMed2000

Correlation of Glut-1 glucose transporter expression with.

Positron emission tomography (PET) with [18F]2-fluoro-2-deoxy-D-glucose (FDG) may show negative results for bronchioloalveolar lung carcinoma. We investigated the correlation of Glut-1 glucose transporter expression with [18F]FDG uptake in non-small cell lung cancer. Thirty-two patients with 34 non-small cell lung cancers (7 bronchioloalveolar carcinomas, 23 non-bronchioloalveolar adenocarcinomas, 3 squamous cell carcinomas, and 1 adenosquamous cell carcinoma) were studied. Final diagnoses were established by histology (via thoracotomy) in all patients. [18F]FDG PET was performed 40 min after i.v. injection of 185 MBq [18F]FDG. For semi-quantitative analysis of [18F]FDG uptake, standardized uptake values (SUVs) were calculated. Glut-1 expression was studied in terms of the immunohistochemistry of paraffin sections using anti-Glut-1 antibody to determine the intensity (0-3) of Glut-1 immunoreactivity and percentage of the Glut-1-positive area. Of seven bronchioloalveolar carcinomas, six (85.7%) were negative for the expression of Glut-1, while only one (4.3%) of 23 non-bronchioloalveolar adenocarcinomas was negative (P < 0.0001). The percentages of Glut-1-positive area, as well as the SUVs, were significantly lower in bronchioloalveolar carcinomas (n = 7) (2.86% +/- 7.56% and 1.25 +/- 0.75, respectively) than in non-bronchioloalveolar adenocarcinomas (n = 23) (54.83% +/- 25.64%, P < 0.0001, and 3.94 +/- 1.93, P = 0.001, respectively). The degree of cell differentiation correlated with the percentage of Glut-1-positive area and SUVs in adenocarcinoma of the lung. Correlations between SUVs and the intensity of Glut-1 immunoreactivity were also significant (intensities 0 and 1, n = 11, SUV 1.47 +/- 0.63; intensities 2 and 3, n=23, SUV 4.78 +/- 2.13; P < 0.0001). The percentage of Glut-1-positive area correlated significantly with SUVs (n = 34, r = 0.658, P < 0.01). Overexpression of Glut-1 correlated with high [18F]FDG uptake. These findings suggest that Glut-1 expression is related to [18F]FDG uptake in non-small cell lung cancer. Glut-1 expression, as well as [18F]FDG uptake, correlated with the degree of cell differentiation in adenocarcinomas, and both Glut-1 expression and [18F]FDG uptake were significantly lower in bronchioloalveolar carcinomas than in non-bronchioloalveolar carcinomas.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMed2000

[A case of trial change in home parenteral nutrition--role of visiting nurses in the care insurance system].

Enteral nutrition is generally selected as a means of administering nutrients during hospital treatment. In the home, however, it is necessary to investigate this from the advantages of care ability and economics. The present patient had long term difficulties in ingesting food. After a gastrostomy in December 1999, the patient returned home with the aim of improving his quality of life, as strongly desired by both the patient and his carer. Since the patient experienced symptoms of diarrhea with enteral alimentation after the gastrostomy, combination oral nutrients (terumiru mini) were diluted and injected after discharge from the hospital. However, this alimentation was not covered by medical insurance, making its cost performance high. At the start of the nursing care insurance system, the patient was certified as having a care level of 5, and the caregiver, who is the patient's eldest son (myelogenic leukemia), had great expectations. However, his burden in the form of purchasing the current alimentation and using the service was extremely difficult financially, so that he was nearly forced to give up hope. Managing the speed of the injection was also a source of great anxiety. Thus, with a view to the lifestyle of the family, an evaluation of their care ability, and analysis of methods able to meet the needs and overcome problems in care crises, as well as adopting the condition that the method be covered by medical insurance, we attempted a change in alimentation and the purchase of an enteral pump. The process of making these changes involved a nutritionist working in cooperation with the doctor, a nurse from a care facility, and a visiting nurse who took the role of coordinator with a medical supply company. The above was possible due to continuous support while confirming a satisfactory care status was being maintained. As a result, we were able to alleviate the economic and psychological burden of care. In the present process, we learned that a care crisis should always be expected, and that we must have the ability to judge the overall needs of the patient while evaluating care ability. Moreover, we discovered that the nursing ability to provide timely, continuing care, including the psychological aspect, is demanded.

Aged↗
PubMed2000

[A survey of current nutrition therapy for the ALS patients in Japanese national sanatoriums].

To evaluate the current nutrition therapy for the patients of amyotrophic lateral sclerosis (ALS) in Japan, we sent questionnaires to 53 Japanese national sanatoriums (hospitals) and were able to collect replies from 42 institutes. In these replies, information on 466 patients (262 inpatients and 204 outpatients) was reported, accounting for about 10% of all Japanese ALS patients. Patients who ate orally were more frequent (p < 0.001, chi 2 test) in the outpatients (76.0%) than in the inpatients (39.7%). When the subjects were limited to non-orally nourished cases, gastrostomy was more frequently performed (p < 0.05, chi 2 test) in the outpatients (49%) than in the inpatients (29.1%), but no significant differences were observed between the above two groups as to the quantity of daily caloric intake; the mode was 1000-1200 Cal. About a quarter of non-orally nourished patients received supplemental sodium chloride, and fewer were supplemented copper and/or zinc. The consent of the patients as well as the clinical findings weighed heavily in the determination of the feeding route. Most physicians were disinclined to encourage tube feeding in the early stage of dysphagia, but were supportive of the operation of percutaneous endoscopic gastrostomy when non-oral nutrition therapy became necessary. Although the efficacy of vitamins for ALS has not been proved, 45 of 58 physicians agreed to subscribe vitamins to their patients, especially vitamin B12, E and C.

Amyotrophic Lateral Sclerosis↗
PubMed1999

Kinetic Study of Lead Adsorption to Composite Biopolymer Adsorbent.

A kinetic study of lead adsorption to composite biopolymer adsorbents was carried out. Spherical and membranous adsorbents containing two biopolymers, humic acid and alginic acid, were used for lead adsorption in dilute acidic solutions. The shrinking core model derived by M. G. Rao and A. K. Gupta (Chem. Eng. J. 24, 181, 1982) was applied to describe the rate process of lead adsorption to spherical adsorbents (average radii of 0.12, 0.15, and 0.16 cm). Furthermore, the shrinking core model was modified and adapted for description of the rate process of lead adsorption to membranous adsorbent (average thickness of 0.0216 cm). The adsorption rate process for the both cases was well described and average apparent lead diffusion coefficients of about 6 x 10(-6) and 7 x 10(-6) cm2 s-1 were found for the spherical and membranous adsorbents, respectively. Copyright 1999 Academic Press.

Journal Article↗
PubMed1999

Long-term result of arterial grafts interposed for arterial defects using the telescoping anastomotic technique: histological and angiographic study.

The arterial grafting model for arterial defects provides an ideal environment for the microvascular telescoping anastomosis because tension of the repaired vessel is maintained low and deformities of the inserted vessel end is minimal due to the thick arterial wall. The left femoral artery was grafted in 46 rats into the defect created in the right femoral artery using the telescoping anastomotic technique at both the proximal and distal anastomoses. The grafts were seen histologically and angiographically 3 to 4 months postoperatively in order to fairly evaluate the telescoping anastomosis in terms of long-term patency, stenosis, and changes in the outer and inner walls at the anastomosis. All arterial grafts were patent 3 to 4 months postoperatively and there were no aneurysms at the anastomoses. Enlargement of the graft diameter was minimal. The rate of stenosis at the anastomosis was smaller than that 1-day postoperatively. Even though the thick arterial wall was initially inserted and the intimal hyperplasia was still present at the anastomosis 3 to 4 months postoperatively, the long-term stenosis was relatively mild due to the atrophy in the overlapped walls.

Anastomosis, Surgical↗
PubMed1999

Suppressed severity of collagen-induced arthritis by in vivo transfection of nuclear factor kappaB decoy oligodeoxynucleotides as a gene therapy.

OBJECTIVE: In both rheumatoid arthritis and collagen-induced arthritis (CIA), the nuclear factor kappaB (NF-kappaB) transcription factor plays a pivotal role in the coordinated transactivation of many cytokines related to pathogenesis. This study investigated whether synthetic double-stranded DNA that show a high affinity for NF-kappaB could be introduced in vivo as "decoy" cis elements to bind the transcription factor and block the activation of such proinflammatory cytokine genes as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha), and thus suppress the severity of joint destruction. METHODS: NF-kappaB decoy oligonucleotides (ODN) were introduced by an intraarticular injection into the bilateral hind ankle joints of CIA rats using the hemagglutinating virus of Japan (HVJ)-liposome method. Joint destruction was evaluated by histology and radiography. IL-1 and TNFalpha levels were assessed by enzyme-linked immunosorbent assay and Northern blot analysis. RESULTS: Using the HVJ-liposome method, the presence of fluorescein isothiocyanate-labeled ODN in the synovium was confirmed until 28 days after intraarticular injection. In vivo transfection of NF-kappaB decoy ODN by an intraarticular injection into CIA rats decreased the severity of hind-paw swelling. Histologic and radiographic studies showed a marked suppression of joint destruction treated by NF-kappaB decoy ODN transfection. This treatment method also suppressed the production of IL-1 and TNFalpha in the synovium of arthritic joints. CONCLUSION: The present results demonstrate that administration of NF-kappaB decoy ODN in arthritic joints of rats with CIA led to an amelioration of arthritis. These findings suggest that intraarticular transfection of NF-kappaB decoy ODN may provide a useful therapeutic approach for the treatment of inflammatory arthritis.

Animals↗