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Biomedical subjects

H Schuster

Publications and source records attributed to H Schuster.

At least 145 records · Page 8Linked to original sources
PubMed1987

Renal excretion of sucrose after transfusion of sucrose containing red cell concentrates.

The elimination of sucrose from plasma and the urinary excretion after transfusions of 394 units of sucrose containing red cell concentrates (RCC) to 108 patients has been studied. 70 mmol sucrose corresponding 3 units of RCC were eliminated from the blood plasma to 90% within 3 hours and excreted by kidneys to 55-80% within 12 hours. The rate of excretion depended on the kidney function. The transfusion of RCC resuspended with the sucrose-rich CSD-AG preservation solution (80 mmol sucrose/l RCC) raised the hemoglobin concentration in patient's blood by 0.5-0.6 mmol/l related to 1 unit of RCC.

Blood Preservation↗
PubMed1987

[Elimination of saccharose from the blood and its renal excretion following transfusion of buffy coat-free erythrocyte concentrates containing disaccharides].

The elimination of sucrose from the blood and its renal excretion was analysed in 108 patients after applying a total of 394 transfusion units (TE), resuspended, buffy-coat-free erythrocyte concentrates (EK) containing 23 mmol of sucrose per TE. In transfusing 3 TE even 90% of the sucrose were eliminated from the blood during the application time and up to 99% within 3 h, nearly 80% were excreted through the kidneys within 12 h. Elimination and excretion were delayed with impaired kidney function. With respect to intravasal elimination of sucrose bilaterally nephrectomized patients have to rely on hemodialysis. Side-effects of sucrose due to extended intravasal and interstitial duration could not be observed in those patients affected with decreased kidney efficiency and after massive transfusions.

Aged↗
PubMed1986

[Is there a hyporeactivity of the vascular wall to atherogenic risk factors?].

The hypothesis is promoted that the arterial vessel can modulate the response to atherogenic factors. Patients with the same index of atherogenic factor may have different degrees of arteriosclerosis. The verification of this thesis needs a prospective study. The second fact described is the diminished experimental arteriosclerosis after treatment with the calcium antagonist Nifedipine (Corinfar).

Aged↗
PubMed1986

[Primary tumors of the retroperitoneal space].

Symptoms, therapy, and prognosis of primary tumours of the peritoneum are reported in this paper, with reference being made to 86 malignant and 24 benign cases. The palpable tumour, impaired condition, and uncharacteristic abdominal pain were major symptoms. Intravenous urography and selective angiography, complementarily flanked and supported by computer tomography and sonography have proved to be important and valuable diagnostic methods. Only 23 per cent of malignant and 62 per cent of benign tumours were operable. Complete extirpation of the tumour was found to be the only promising therapy. Postoperative lethality after total extirpation amounted to ten per cent. Combination of surgical with radiotherapeutic approaches has proved to be favourable. The five-year survival rate accounted for 9.8 per cent or 44 per cent, following radical surgery.

Adolescent↗
PubMed1984

Cloning of the dnaB gene of Escherichia coli: the dnaB gene of groPB534 and groPB612 and the replication of phage lambda.

Fragments of the E. coli chromosome that carry the dnaB groPB534 or groPB612 alleles have been cloned into a cosmid vector. The resulting recombinant plasmids contained the genes uvrA, groP (B534 or B612), and lexA. Further subcloning into high copy number plasmids, during which the uvrA and lexA genes were removed successively, yielded a groPB534 and groPB612 DNA fragment of about 2.4 kb each. Both fragments contained an overlapping 1.8 kb segment of DNA in which the sites of all restriction enzymes tested were identical. The size of these dnaB gene fragments were further delimited by deletion analysis. In E. coli groPB534 in which lambda wild-type and lambda pi A mutants do not replicate (Georgopoulos and Herskowitz 1971) phage replication is rescued if the strain contains the groPB534 gene on high copy number plasmids. On the contrary, in E. coli groPB612, which is temperature-sensitive for its groP character, replication of lambda and lambda pi A is abolished at 30 degrees C if the strain contains the groPB612 recombinant plasmid. On the other hand, replication of lambda pi B remains unaffected whether or not the groP strains harbor the isogenic dnaB gene-containing plasmid. The results suggest that within the cell not only the quality but also the relative amounts of dnaB and lambda P protein are crucial for lambda phage replication.

Bacterial Proteins↗
PubMed1983

The dnaC protein of Escherichia coli. Purification, physical properties and interaction with dnaB protein.

E.coli dnaC protein was purified to near-homogeneity in using a dnaC complementation assay [S. Wickner, I.Berkower, M.Wright, and J. Hurwitz (1973) Proc. Natl. Acad. Sci. USA 70, 2369-2373]. Purification was achieved by taking advantage of the hydrophobic interaction of dnaC protein with aliphatic and aromatic matrixes and with Brij58 as stabilizing agent. A sedimentation coefficient for the dnaC protein of 2.6 S corresponding to a molecular weight of approximately 26,000 was estimated from glycerol gradient centrifugation. A polypeptide molecular weight of 28,000 was determined by densitometry on a denaturing gel. In the presence of ATP the dnaC protein forms a complex with dnaB protein [S. Wickner and J. Hurwitz (1975) Proc.Natl.Acad.Sci. USA 72, 921-925]. For the dnaB . dnaC complex a sedimentation coefficient of 14.5 S was measured by glycerol gradient centrifugation, indicating a molecular weight of about 400,000. The ratio of the dnaC and dnaB polypeptides in the complex is approximately 1, as determined on a denaturing gel. It is suggested that the complex consists of the dnaB protein hexamer and six dnaC polypeptides amounting to a calculated molecular weight of about 450,000.

Bacterial Proteins↗
PubMed1981

Stabilization by ATP and ADP of Escherichia coli dnaB protein activity.

The effect of adenine ribonucleotides on the stability of Escherichia coli dnaB protein in cellular crude extracts was studied. Stabilization of dnaB protein by ATP or ADP, but not by AMP, was manifested in that (i) the activity and yield of wild type dnaB protein is enhanced in the presence of ATP, (ii) the dnaB protein of E. coli dnaB mutants, such as groPB and dnaB252/ColE1::dnaC+, which is inactive in a dnaB complementation assay, can be isolated in active form in the presence of ATP or aDP, (iii) ATP or ADP protect the dnaB protein of an E. coli dnaBts mutant from inactivation at 37 degrees C, and (iv) inactive groPB and dnaBts protein can be reactivated partially by ATP. Thus, the stabilizing effect of ATP and ADP can be exploited for the isolated of otherwise inactive or labile mutant dnaB proteins.

Adenosine Diphosphate↗
PubMed1981

The isolation and characterization of escherichia coli dnaB::Tn10 insertion mutations.

Exploitation of the ability of the ban protein encoded by phage P1 to compensate for dnaB-defective host mutations, allowed the isolation of dnaB::Tn10 insertion mutations. The presence of P1bac prophage was required for survival of dnaB::Tn10 mutants, and such lysogens were cryosensitive. The insertions were shown to map in dnaB by transduction and this was confirmed by complementation analysis. The dnaB::Tn10 (P1bac) strains were non-permissive for lambda growth but did support the growth of lambda-dnaB+ specialized transducing phage. No antigenically active dnaB product could be detected by immunologic assays using either of two methods. In addition, it was shown that the observe cryosensitivity of P1bac suppression was a direct result of reversible inactivation of the ban protein at low temperature.

Bacterial Proteins↗