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Biomedical subjects

H Schmechta

Publications and source records attributed to H Schmechta.

At least 37 records · Page 2Linked to original sources
PubMed1984

[Determination of alpha-1-fetoprotein in maternal serum dried on filter paper by an enzyme immunoassay].

The suitability of different carrier materials for absorption of serum was investigated with respect to a centralised screening for AFP serum levels in pregnant women, which needs a transmission of the samples. AFP was quantified in an indirect two-site binding enzyme immunoassay. AFP concentrations of native serum and of the eluate from the samples dried on filter paper were in a good agreement with a coefficient of correlation of 0.902. But storage of dried serum samples should not exceed one week because a two week storage diminished the coefficient of correlation to 0.766 and AFP concentrations of paper dried samples were overestimated.

Female↗
PubMed1984

No inhibition of the lymphocyte transformation by pregnancy-associated alpha 2-glycoprotein (PAAG).

Fractions of retroplacental sera containing pregnancy-associated alpha 2-glycoprotein (PAAG) inhibited the lymphocyte transformation. The inhibitory effect did not correlate to the amount of this protein. It remained even after a specific depletion of the PAAG without altering the composition of the other proteins in the sera fractions. These results rule out a suppressive activity of the PAAG in the lymphocyte transformation.

Humans↗
PubMed1983

[Enzyme immunoassay of alpha 1-fetoproteins in patients with testicular tumors].

The use of a newly developed immunoassay for alpha-1-protein in testicular tumours is described. With 2 ng AFP/ml serum the lower detection group of the test is in the physiological range. Of 39 non-seminomatous testicular tumours, 59% had a high AFP-serum level post-operatively, of 51 on initial diagnosis 54%; 12 seminomas remained AFP-negative. Checking the AFP level ist valuable for diagnosis, staging and prognosis of all testicular tumours.

Adult↗
PubMed1982

Quantitation of a pregnancy associated globulin in the rat by two-site binding enzyme immunoassay.

An indirect two-site binding enzyme immunoassay has been developed to quantify the pregnancy associated globulin (PAG) in rats. Anti-PAG from guinea pig is adsorbed to solid phase and binds serum-PAG, which then reacts with its remaining free determinants with anti-PAG from rabbit. Sheep anti-rabbit IgG, labeled with horse radish peroxidase (HRP), binds to the rabbit anti-PAG antibody and the enzymic activity of the solid phase is measured by incubation with the appropriate chromogenic substrate. The optical density is directly related to the quantity of PAG to be measured. Reproducible results are obtained in the range from 5 to 200 micrograms/l. The test detects 12 fmoles of PAG. Female rats show a 1000-fold higher serum level of PAG than male rats. Strain differences of the distribution of the PAG serum concentration were only found between male rats.

Animals↗
PubMed1981

Effect of IgG-horseradish peroxidase conjugates purified on Con A-Sepharose upon sensitivity of enzyme immunoassay.

Antibodies coupled to horseradish peroxidase (HRP) were purified by gel filtration and by combination of affinity chromatography and gel filtration. Binding kinetics to solid phase bound antigens were studied of the antibody-enzyme conjugates in the presence and in the absence of unlabelled IgG. The detection limit in enzyme immunoassay was found to be lowered by factor 16 after elimination of unlabelled IgG from the conjugate.

Animals↗
PubMed1980

Isolation of a pregnancy-associated globulin (PAG) from the rat and preparation of a specific antiserum (anti-PAG).

Isolation of a glycoprotein from serum of pregnant rats has been accomplished by ammonium sulphate precipitation, gel filtration, ion exchange and affinity chromatography. Low concentrations of the protein were detectable in the serum of some of the male rats tested, while somewhat higher concentrations were detected also in the serum of non-pregnant female rats. No relationship could be established between this protein and the well known pregnancy-specific proteins or the alpha 2-acute phase-macroglobulin of the rat, whereas evidence was obtained for a strong cross-reaction with a serum protein of pregnant mice.

Animals↗
PubMed1979

[Separation of human X- and Y- chromosome-bearing spermatozoa by column chromatography (author's transl)].

Separation of X- and Y-chromosome-bearing spermatozoa has been attempted using Sephadex gel filtration. Fractions rich in X-chromosome-bearing spermatozoa were eluted in dependence on length of separation distance and on speed of elution. Spermatozoa remaining at the gel showed a higher percentage of Y-bodies compared with the untreated control. Sedimentation pattern and amount of eluted cells is influenced by eluation conditions.

Cell Separation↗