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Biomedical subjects

H Schenk

Publications and source records attributed to H Schenk.

At least 73 records · Page 4Linked to original sources
PubMed1984

[Emergencies in oncology].

In clinical oncology emergency situations are either manifestations of the underlying disease or effects of the treatment. The successful treatment of emergency situations is not only important in diseases in which cure is possible. It contributes also essentially to an optimal palliation for not curable disease states in oncology. Some special examples from the point of view of internal medicine are briefly discussed.

Allopurinol↗
PubMed1984

[Acute disorders of hemostasis].

Acute acquired hemostatic disorders are of widespread origin. Coagulation disorders (severe liver damage), platelet disorders (impairments of platelet function and number, immunologic and/or toxic myelosuppression) or various combinations (e.g. disseminated intravascular coagulation). According to the various etiologic factors different diagnostic and therapeutic approaches are appropriate.

Antithrombin III↗
PubMed1984

Pemphigus in the dog: comparison of immunofluorescence and immunoperoxidase method to demonstrate intercellular immunoglobulins in the epidermis.

In 3 dogs with pemphigus vulgaris and 4 dogs with pemphigus foliaceus, intercellular immunoglobulins were demonstrated in the epidermal stratum spinosum. The immunofluorescence technique on cold ethanol-fixed and paraffin-embedded tissue sections was compared with the immunoperoxidase method on formalin-fixed paraffin-embedded tissue sections. The results of both methods were identical. However, the advantage of the unlabeled antibody-enzyme method was that the same formalin-fixed tissue specimens could be used for conventional light microscopy, as well as for immunohistologic studies.

Animals↗
PubMed1984

[Contents and patterns of gangliosides in the membranes of human erythrocytes].

10-20ml erythrocytes of 17 healthy subjects were separated from leukocytes by passage through cotton. The erythrocytes were haemolyzed in hypotonic buffer and the ghosts isolated by centifugation. Lipid extraction was performed and the gangliosides purified by molselect chromatography. Fractionation of gangliosides was carried out by thin layer chromatography. The quantitative and qualitative determination was performed by thin layer scanning with a High Speed TLC-Scanner Shimadzu CS 920. The sensitivity of the method was about 100 ng lipid bound neuraminic acids. The concentration of neuraminic acids in the total gangliosides was about 14 microgram/20 ml erythrocytes. The distribution of the 4 different ganglioside fractions which could be detected after staining with Svennerholm reagent were: GM3=16%, GM1=52%, GD1a=22%, GT1=90%.

Chromatography, Thin Layer↗
PubMed1981

Purification and properties of the membrane-bound acetylcholinesterase from adult rat brain.

The membrane-bound acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) from adult rat brain has been purified to homogeneity using sequential affinity chromatography on Con A-Sepharose and on dimethyl-aminoethylbenzoic acid-Sepharose 4B followed by DEAE-cellulose chromatography. The yield of the purified enzyme (specific activity: 3068 U/mg protein) is higher than 50%. Polyacrylamide gel electrophoresis in the presence of Triton X-100 gives only one band with acetylcholinesterase activity. With the exception of electrofocusing and pore gradient electrophoresis, where a multiple band pattern was detected (which seems to be artefactual), the enzyme appears to be homogeneous. Gel filtration and sucrose density gradient centrifugation in the presence of Triton X-100 give only one symmetrical peak, with a calculated molecular weight of 328 000. Since polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS) and mercaptoethanol gives only one band with a molecular weight of 74 500, a tetrameric structure can be postulated for the membrane-bound acetylcholinesterase from rat brain.

Acetylcholinesterase↗
PubMed1981

[Clinical studies of familial hereditary factor VII deficiency (author's transl)].

The discovery of a severe factor VII deficiency with increased bleeding tendency resulted in investigations of 22 members of the family. In the propositus and in two of his siblings a severe hypoproconvertinemia was demonstrated, a partial deficiency was found in ten persons. Studies of the family confirmed that this disorder is transmitted by an autosomal gene with intermediate penetrance. The mutated gene produces a severe deficiency in the homozygote and partial deficiency in the heterozygote. The parents of the homozygote patients were consanguineous. Hemorrhagic diathesis was noted only in patients with a severe factor VII deficiency. Causes for the variability of the clinical manifestations are discussed.

Adult↗
PubMed1980

[Effect of the antirheumatic drug Sulindac on thrombocyte function (author's transl)].

The effect of a treatment with the antirheumatic drug Sulindac (from 200 to 400 mg/day over 4 weeks) on thrombocyte function (bleeding time, platelet retention, platelet aggregation induced with suitable concentrations of ADP, epinephrine, collagen and Ristocetin) on thrombocyte count and thromboplastin time was investigated in 20 patients. In 10 healthy volunteers collagen-induced platelet aggregation was measured after oral application of 200 mg Sulindac. Analysis of the data obtained by the tests revealed no unwanted or harmful effects of Sulindac on platelet functions, platelet count and prothrombin time. No significant difference between the data before and during treatment was found. No medical drop out was registrated.

Collagen↗
PubMed1980

Comparison of myocardial oxygen consumption indices in man.

Hemodynamic indices of myocardial oxygen consumption (MVO2) were examined in 13 patients with coronary heart disease. The specific aim of this study was to investigate whether clinical results agree with experimental findings in animals. During hemodynamic and respiratory steady-state conditions at rest, the MVO2 (7.6-14.2 ml/min/100 g) was measured directly by myocardial blood flow (argon method) and arterio-coronary venous oxygen content difference. MVO2 was compared with five indirect indices of MVO2. A complex additive parameter consisting of five components of MVO2 had the highest correlation with MVO2 (r = 0.97), as was also demonstrated in a recent experimental study at maximum changes in hemodynamics and MVO2. More easily measurable predictors of MVO2 such as tension-time index (r = 0.923), product of mean systolic aortic pressure and square root of heart rate (r = 0.928), pressure rate product (r = 0.915), and triple product (r = 0.941) were less closely correlated with MVO2. The lower correlations of the readily obtainable indices of MVO2 are probably related to their failure to incorporate factors such as contractility and ventricular dimensions, which are known to exert an important influence on MVO2. The excellent correlation of the hemodynamic additive parameter with MVO2 supports the theoretical concept and the implications of the experimental study. The accurate prediction of MVO2 is based on adequate measurement of MVO2 for the velocity of tension development and maintenance of tension during systolic ejection period, both of which are integrated in the additive index.

Adult↗