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Biomedical subjects

H Sawada

Publications and source records attributed to H Sawada.

At least 577 records · Page 32Linked to original sources
PubMed1977

Isolation of apoprotein from bovine lung surfactant material by sodium deoxycholate.

Aqueous solutions of bovine lung surfactant material were solubilized with a high concentration of sodium deoxycholate and the protein moiety could then be separated from the mixed lipid-sodium deoxycholate micelles by gel filtration in the presence of a micellar concentration of sodium deoxycholate of 10 mM. The lipid-free protein showed only one detectable peak in the gel filtrate and the sedimentation rate of this protein was 12.1 S. Sodium dodecyl sulfate disc gel electrophoresis showed essentially the same pattern as did the protein extracted directly from the bovine lung surfactant material with organic solvents, of which the major component has a molecular weight of 36 000. This protein should be the main apoprotein of lung surfactant material, and the main band in the sodium dodecyl sulfate disc gel electrophoresis, with a molecular weight of 36 000, would constitute the major protein subunit.

Animals↗
PubMed1977

RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells.

A high molecular weight RNA-reverse transcriptase complex in the culture media of peripheral leukocytes obtained from two Japanese patients with myeloma-leukemia was detected by demonstration of a 3H-uridine peak and a peak of DNA polymerizing activity banding at a density of 1.15-1.19g/ml. The enzyme in the complex was able to utilize poly(rA)-d(pT)10 or poly (rC)-d(pG) 12-18, but not poly (dA)-d(pT) 10 or (dT) 12-18 as template-primers. The sucrose density sedimentation analysis revealed that RNA in the complex sedimented at a location of approximately 50s and 20-30s.

Adult↗
PubMed1977

Sodium dodecyl sulfate-disc gel electrophoresis patterns of bovine lung surfactant.

Bovine lung surfactant material, isolated by three steps of centrifugation from lung washings, had a phospholipid: protein ration of 4.98+/-S.D. 0.62 mumol/mg. Sucrose continuous density gradient centrifugation showed only one visible band at a density of 1.081 g/ml. Sodium dodecyl sulfate-disc gel electrophoresis patterns of the apoprotein extracted from this material revealed that the main component (70-80%) of the protein subunits of bovine lung surfactant material has a molecular weight of 36 000. It was suggested that the apoprotein of bovine lung surfactant material exists as a combination of a few protein subunits, most of which have a molecular weight of 36 000.

Animals↗
PubMed1976

Isolation and identification of benzodiazepine drugs and their metabolites in urine by use of Amberlite XAD-2 resin and thin-layer chromatography.

We used the method described here to detect and identify seven benzodiazepine derivatives--diazepam, chlorodiazepoxide, nitrazepam, cloxazolam, oxazolam, oxazepam, and medazepam--and their metabolites in the urine of rabbits given the seven drugs orally. We column-chromatographed 25-ml samples of urine on Amberlite XAD-2. The drugs and their metabolites in the urine were adsorbed by the resin, irrespective of urinary pH, and upon successive elution with methanol and ethyl acetate/methanol/acetic acid (90/10/0.1 by vol) they could be separated and extracted from the normal components of urine with satisfactory analytical recovery. The conjugated metabolites were then enzymatically hydrolyzed and the hydrolysate was extracted into ethyl acetate and the extract thin-layer chromatographed to detect and identify each drug and each of its metabolites. In an experiment in which the urine of human subjects given 5 mg of nitrazepam orally was analyzed by this method, the metabolites of nitrazepam in the 24-h urine could be identified satisfactorily.

Animals↗
PubMed1976

Caffeine potentiation on lethality of L-1210 cells treated with neocarzinostatin.

The caffeine potentiation on lethality of L-1210 cells treated with Neocarzinostatin was studied. Colony formation of L-1210 cells treated with Neocarzinostatin was significantly decreased by incubation of the cells for 48 hr with 0.5 mM of caffeine which alone did not affect the growth rate and colony-forming ability of the cells. These results indicate that Neocarzinostatin may induce repairable damage in DNA of L-1210 cells.

Animals↗
PubMed1976

Effect of a nitrofuran derivative (AF2) on the immune response of mice.

The effect of 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2), which had been widely used in Japan as a food preservative, on the immune response of mice was analyzed, since the drug has been known to reveal very potent mutagenicity but fairly weak carcinogenicity. Although AF-2 suppressed the primary immune response of mice under limited experimental conditions, in general, it augmented the immune response of mice, especially the production of immunological memory. It was found by hapten-carrier system that activities of both the T-cell lineage and the B-cell lineage were enhanced by the administration of AF-2, but the activation of the T-cell lineage seemed to be primary because the chemical did not affect the response of mice to a T cell-independent antigen epsilon-2,4-dinitrophenyl-L-lysyl-dextran T2000 (DNP50-dextran T2000). AF-2 did not prevent the suppressive effect of 7,12-dimethylbenza[a]anthracene (DMBA) on the immune response but it suppressed both the primary and the memory production in concert with DMBA, suggesting that the augmenting effect of AF-2 on the T-cell lineage may be in a different way from that of immunopotentiators such as lentinan.

9,10-Dimethyl-1,2-benzanthracene↗