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Biomedical subjects

H Sauer

Publications and source records attributed to H Sauer.

At least 271 records · Page 15Linked to original sources
PubMed1987

Cytostatic drug testing in human leukemias by means of multiparametric flow cytometry.

Human bone marrow cells from 20 patients as well as the permanent human B-cell lines RPMI 1788, Raji, Daudi, T-cell lines Molt, CEM, Jurkat and the promyelocytic line HL 60 were assayed by means of a newly developed in vitro flow cytometric cytostatic drug assay. The cells were exposed to cytosine-arabinoside, L-asparaginase, daunorubicin, prednisone or vincristine. Surviving cells were stained after an incubation period of 2 to 7 days with esterase and pH-indicator dye ADB (1,4-diacetoxy-2,3-dicyanobenzene), dead cells with DNA-dye PI (propidium iodide). Dose-response curves were established using percent surviving cells. It was possible to evaluate bone marrow samples from 16 out of 20 patients. Seven samples were leukemic (acute myeloid leukemia (AML) n = 6, Non-Hodgkin's Lymphoma (NHL) n = 1). Nine samples were from patients either in complete remission or with benign diseases. Daunorubicin and cytosine-arabinoside were cytotoxic in both groups, whereas vincristine was effective mainly in the leukemic group (p less than 0.05). There was significant heterogeneity in the reactivity of AML-marrow cells from different patients to different drugs. The cell lines exhibited different patterns of sensitivity. Vincristine arrested cells in G2/M-phase, cytosine-arabinoside caused an increase of cells in the S-phase.

Antineoplastic Agents↗
PubMed1987

[Results following surgical treatment of ligament ruptures of the ankle joint in children].

A follow-up study was done in 49 children one to five years after surgical repair of traumatic rupture of the fibular ligaments of the ankle joint. Surgery has been performed when the unstable ankle joint showed radiologically a difference in the supinatory subluxation of more than 7 degrees in comparison to the opposite side. 30 children were free of complaints and showed identical stability of both ankle joints. Further eleven patients had minor complaints but the ankle joints were stable, clinically and radiologically. Four patients without complaints had a slightly differing weakness of the ankle joint. Further four patients had substantial complaints. Three of them also showed radiologically instability of the joint. Ruptures of the fibular ligament in childhood are not infrequent. Early operative treatment obviously gives better results when compared with those of conservative plaster cast therapy. The radiologically confirmed difference in supinatory subluxation of the traumatised ankle joint in comparison to the other side provides an excellent parameter for the indication to surgery.

Adolescent↗
PubMed1987

[Toxicity of the CYVADIC modification in patients with soft tissue sarcomas or malignant mesotheliomas].

CYVADIC (cyclophosphamide + vincristine + adriamycin + dacarbazine = DTIC) is frequently used in the treatment of patients with soft tissue sarcomas or malignant mesotheliomas. As a modification of the protocol originally published by Gottlieb, the interval between two cycles was extended to 4 weeks for better bone marrow regeneration and vincristine was given only once instead of twice per cycle. A total of 237 cycles of this modified CYVADIC protocol resulted in tolerable toxicity (except nausea and vomiting) and therapeutic results comparable to the original protocol (6/22 complete remissions and 6/22 partial remissions in sarcomas; 1/5 partial remission in mesotheliomas). Hematotoxicity was cumulative after 6 CYVADIC cycles. In contrast to the original Gottlieb scheme there were no severe infections such as pneumonia or sepsis. Neurotoxicity was weak and reversible; cardiotoxicity was not observed. Compared to the protocol originally suggested by Gottlieb, the modified CYVADIC regimen appears to be as effective but less toxic.

Adolescent↗
PubMed1987

[Hygiene certification of the Society for Proper Quality of Linen Care, Incorporated].

This is a survey of textile-technical requirements, of quality controls, of the hygiene certificate as well as of the relevant valid legal provisions, regulations and directions. The test-list for works inspection is quoted. The hygienic-bacteriological control methods as well as the checking methods are described by means of selected examples.

Bedding and Linens↗
PubMed1987

[Transplantation antigen specific rosette test and its inhibition with autologous serum--a new immunologic method for predicting rejection crisis following allogeneic organ transplantation].

A combination of transplantation-antigen-specific rosette test with inhibition of rosette formation, using autologous serum, was used in postoperative follow-up checks on patients who had undergone allogeneic kidney transplantation and proved successful in early diagnosis or prediction of rejection episodes even before clinical manifestation. The numbers of antigen-detecting and antigen-binding cells differed considerably from each other, depending on patients who had undergone kidney transplantation. Almost complete inhibition of antigen detection was generally achieved by addition of patient serum. If sensitization to alloantigen in excess of the limit value (30 RBZ/10(3) KHZ) is accompanied by rosette formation with autologous serum addition in excess of about 18 RBZ/10(3) KHZ, clinically relevant rejection can be expected to occur after another two or three days and will have to be treated by immunosuppressives. Such partial or even complete elimination of rosette inhibition is usually only of short length (one day) and has rarely been observed to last longer. No rejection calling for treatment will develop, on the other hand, as long as blocking serum factors provide for high-stability inhibition of any bond of transplantation antigen (rosette formation). Constantly low rosette formation with serum addition and without was exhibited by control persons (blood donors, patients with cerebrocranial trauma) in daily examinations. Limit values of 18 or 30 RBZ/10(3) KHZ were not surpassed.

Graft Rejection↗
PubMed1987

Relation between cell cycle stage and the activity of DNA-synthesizing enzymes in cultured human lymphoblasts: investigations on cell fractions enriched according to cell cycle stages by way of centrifugal elutriation.

Undifferentiated human lymphoblasts (culture LS-2) were separated according to cell size during their exponential growth phase by way of centrifugal elutriation. The cell fractions thus obtained were characterized in terms of different cell cycle stages by flow cytometric measurement of their deoxyribonucleic acid (DNA histogram), the [3H]thymidine labeling index, and by determining the rate of [3H]thymidine incorporation. In these cell fractions the activities of thymidine kinase, thymidylate synthase, DNA polymerase, dihydrofolate reductase, methionine synthase, and hexokinase were determined. The results showed that all the enzymes investigated exhibited activities in all cell fractions. With the exception of DNA polymerase, all of the enzymes exhibited the lowest level of activity in the fraction containing the highest proportion of G0 + G1 phase cells (fraction 2); the activity of thymidine kinase was particularly low. This would suggest that thymidine kinase is not active in G0 + G1 phase cells and that the activity measured in fraction 2 is perhaps attributable to contamination of this fraction by S and G2 + M phase cells.

5-Methyltetrahydrofolate-Homocysteine S-Methyltran↗
PubMed1986

[Problems of gastroesophageal reflux in childhood].

Gastro-esophageal reflux is common in infancy. However, in most of those children reflux disappears spontaneously at the end of the first year or during the second year of life. Thus, the authors suggest the hypothesis that gastro-esophageal reflux in infancy represents a delay in maturation of central structures for the motor function of the esophagus. Therefore conservative therapy during infancy is the treatment of choice for most of the infants. Additional factors however, such as severe cerebral or neurological damage, esophageal atresia, failure to growth and esophagitis make an early operative intervention often necessary. Likewise operative therapy is the treatment of choice in older children with symptoms of esophagitis or stenosis. Different diagnostic procedures are discussed briefly.

Child Development↗
PubMed1986

Experiences in the treatment of esophageal atresia with Rehbein's olive technique.

During the past few years four children with esophageal atresia without lower fistula were treated with the thread-and-olive method according to Rehbein. Our experiences indicate that excellent results can be achieved if the thread is inserted early, endoscopically, and olive bougienage is performed at short intervals. Tracheoscopy should be mandatory prior to the start of treatment, since we observed an upper fistula in three of four cases. Endoscopic thread insertion may lead to perforation of the main bronchus; control tracheo-bronchoscopy must therefore be done immediately following insertion. Perforation of the main bronchus turned out to be harmless, so the maneuver can be repeated after a few days. Two of the four infants died of congenital malformations. Therapy was not related to the fatal outcome in either case. One of the survivors has practically normal esophageal motor function with proven propulsive peristalsis. Esophageal stenosis had to be resected in the second survivor; this was followed by massive gastroesophageal reflux with esophagitis and disturbed gastric motility, rendering subsequent fundoplication and pyloroplasty necessary.

Esophageal Atresia↗