Biomedical subjects

H Satow

Publications and source records attributed to H Satow.

4 recordsLinked to original sources

PubMed1993

Expression of the mC26 gene encoding GlyCAM-1 in the lactating mouse mammary gland.

We previously reported the partial sequence of a cloned genomic DNA, mC26, which codes for a protein highly expressed in the lactating mouse mammary gland [mC26: Satow et al. (1986) J. Biochem. 99, 1639-1643; partial sequence: Kawamura et al. (1987) J. Biochem. 101, 103-110]. In this study, we sequenced the EcoRI-HindIII fragment (5,394 bp) of this gene and found that this gene contains a sequence completely (100%) homologous to the cDNA sequence currently reported to code for GlyCAM-1, a putative ligand for L-selectin. We show by means of an RNA protection assay that the mRNA of this gene is expressed in the mammary glands of lactating mice as well as in the lymph nodes. Semi-quantitative analysis of expression of the mC26 gene in the mammary glands revealed that the amount of mRNA was not detectable in the early stage of pregnancy, increased in the late stage, and remained quite abundant during lactation. The potential role of this gene highly expressed in the mammary gland in a stage-specific and tissue-specific manner is discussed.

Animals↗

PubMed1987

Modulation of the transferred mouse 26K casein gene in mouse L cells by glucocorticoid hormone.

The cloned 26K casein gene was transferred to mouse L-cells and its expression was measured by Northern blot hybridization. When the lambda clone with 5'- and 3'-flanking sequences was transferred, transcripts were detected without glucocorticoid, but in the presence of glucocorticoid, the level of the transcripts of heterogeneous sizes increased and their pattern was similar to that observed in the mammary glands of non-lactating mice. When the 6.7 kb EcoRI fragment containing most of the coding region was transferred, putative precursor and mature mRNAs were detected without glucocorticoid. Surprisingly, with the addition of glucocorticoid, the level of the transcripts greatly decreased. The presence of multiple sequences responsible for glucocorticoid receptor binding was detected in the 5'-flanking region of the gene in a competition assay using the subfragments of casein gene and on sequence analysis. These results suggest that the 26K casein gene has multiple regulatory domains which interact with glucocorticoid receptors, and these domains may play different roles in the regulation of the casein gene.

Animals↗

PubMed1986

Post-transcriptional control of 26 k casein genes during lactogenesis in mouse mammary glands.

Stage-specific gene expression of 26 k casein, a major milk protein, was examined in mouse mammary glands. Mature mRNA (1.1 kb) with putative precursor RNA (k kb) was detected in the lactating gland. In contrast, the putative precursor RNA (5 kb) was also detected in the mammary glands of virgin or weaned mice when milk was not produced, although mature mRNA was not detected. The RNA transcripts showing heterogeneous size distribution were abundant in the nonlactating stages. These RNAs are shown to be transcribed in the opposite direction to the mature mRNA. These results suggest the regulation at the post-transcriptional level in the synthesis of caseins in the mammary glands.

Animals↗

PubMed1985

[Casein gene].

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Animals↗