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H Sarles

Publications and source records attributed to H Sarles.

At least 127 records · Page 7Linked to original sources
PubMed1985

Alcohol and the integrity of the pancreas.

Alcohol may affect the integrity of the pancreatic parenchyma, as seen in alcoholic pancreatitis, some cases of chronic alcoholism without clinical pancreatitis, and experimental studies. The composition of pancreatic juice may reflect some of these changes. One type of parenchymal alteration is the loss of differentiative features of acinar cells, so that they take on the characteristics of ductular cells. Concomitant fibrosis completes the formation of the tubular complexes found in association with alcoholic chronic pancreatitis. Sustained alcohol intake may produce the accumulation of lipid droplets in parenchymal cells, some of which may be shown to be within the rough endoplasmic reticulum of acinar cells. Epithelial cells may undergo mucous metaplasia. The epithelial-basal lamina barrier frequently is breached in the area of intraluminal aggregates, with or without obvious inflammation in the immediate area. Loss of barrier function may lead to interaction among components of the external compartment (lumen) and the internal compartment (stroma). Increased levels of blood proteins and glycosaminoglycans in the juice, enzyme activation, fibrin formation, and complement activation are potential consequences of barrier loss. Increased lactoferrin levels could result in part from the activity and degranulation of polymorphonuclear leukocytes.

Alcoholism↗
PubMed1985

The pancreas.

Explore the source record for details and available documents.

Adenocarcinoma↗
PubMed1985

[Spontaneous portacaval anastomoses by veins of the falciform ligament of the liver in cirrhosis. Their ultrasonic exploration].

The aim of this work was to study the portacaval shunts described by Sappey in the falciform ligament of the liver in the cirrhotic patient. Twenty men and 8 women (mean age: 63 years) were studied by ultrasonography. Ascites was present in 20 patients. Venous anastomoses through the falciform ligament were visualized in 16 of the 28 patients. When present, they were usually multiple (1 to 4) with a diameter of about 10 mm. In 8 patients, a large communication with one of the main portal branches was seen. In the same patients, gastroesophageal shunts were always present but umbilical and splenorenal anastomoses were found only in 3 and in 4 patients respectively. This emphasizes the importance of such shunts which are in part intrahepatic. Ultrasonic exploration of these shunts should be routine in the evaluation of collateral circulation in cirrhosis.

Aged↗
PubMed1985

[Monoclonal antibody analysis of human pancreatic juice components related to the pancreatic calculi protein].

A CaCO3-crystal growth inhibitor has recently been isolated from the calculi of patients affected by pancreatic lithiasis. It is a phosphoglycoprotein, with a molecular weight of 14,000, whose probable physiological role is the stabilization of exocrine pancreatic secretion which is supersaturated with respect to CaCO3. In order to isolate this inhibitor from human pancreatic juice, monoclonal antibodies to the protein were prepared and an immunoadsorbent column was developed. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of proteins fixed by the immunoadsorbent reveals the form having a molecular weight of 14,000, in addition to other protein bands which have higher molecular weights (16,000, 16,800, 18,000, and 18,800). All of these different proteins are also recognized by a monospecific polyclonal antibody to the 14,000 molecular weight form. Using the same monospecific polyclonal antibody only one messenger RNA coding for this inhibitor has been demonstrated. Thus, this heterogeneity might be explained by post-translational modifications.

Antibodies, Monoclonal↗
PubMed1984

The molecular characteristics of a human pancreatic acidic phosphoprotein that inhibits calcium carbonate crystal growth.

A CaCO3-crystal-growth inhibitor was isolated from human pancreatic stones by using EDTA demineralization, followed by DEAE-Trisacryl chromatography. The isolated inhibitor was found to be a phosphoglycoprotein with Mr 14017 and having an unusual chemical composition. It is characterized by a high (42%) acidic amino acid content, but lacks methionine and gamma-carboxyglutamic acid. The protein contains 2.65 mol of P/mol of protein, as phosphoserine (2 mol) and phosphothreonine (0.5 mol). Isoelectric focusing of the protein yields one major band corresponding to an isoelectric point of 4.2. Immunochemical quantification of the crystal-growth inhibitor in pure pancreatic juice reveals that it constitutes 14% of the normal exocrine secretion. Our findings demonstrate that this is a novel secretory protein, which has no enzymic activity and which maintains pancreatic juice in a supersaturated state with respect to CaCO3.

Amino Acids↗
PubMed1984

[Localized Caroli's disease associated with mucosecreting papillary adenocarcinoma. Value of hepatic resection].

A new case of carcinoma arising in Caroli's disease in a 48-year old male patient is reported. Since dilatation of the bile ducts was localized to the left lobe, left hepatectomy was performed. Histological examination showed that the tumour was a papillary mucoid adenocarcinoma. There was no recurrence of cholangitis while the patient survived. This case and a review of the literature shows that left hepatectomy suppresses repeated attacks of cholangitis in patients with Caroli's disease restricted to the left lobe, and that the incidence of carcinoma in Caroli's disease is higher than was formerly believed, which should encourage early surgical treatment when the disease is localized in the left lobe.

Adenocarcinoma, Mucinous↗
PubMed1984

Influence of environmental conditions on exocrine pancreatic response to intravenous injection of ethanol or 2-deoxyglucose in the dog.

When dogs have free access to the outside, an intravenous injection of ethanol depresses secretin-stimulated exocrine pancreatic secretion by a vagally mediated mechanism. This was shown in two separate series of six and seven dogs each. When dogs were kept in air-conditioned windowless kennels, the response to a meal was unchanged but the response to ethanol was reversed to stimulation. In four dogs, ethanol 1 g/kg was given during a secretin infusion. Three months after changing from open to closed kennels the inhibition (-86% for protein output) was still present, but after 6 months ethanol produced a stimulation (+62%) of pancreatic secretion. This increase was abolished, but not reversed, by keeping the animals outside during the day for four weeks, whereas after three months there was a partial restoration of the inhibitory effect (-39%). In contrast, changing from an open to a closed kennel changed the initial response to 2-deoxy-D-glucose (2-DG), 100 mg/kg, from stimulation to inhibition. These results suggest that environmental conditions affect the cranial regulation of pancreatic secretion.

Animals↗
PubMed1984

Annular pancreas. Report of one case associated with acute pancreatitis and diagnosed by endoscopic retrograde pancreatography.

Diagnosis of annular pancreas is possible by endoscopic retrograde cholangiopancreatography (ERCP) only when the branch encircling the duodenum is connected to the pancreatic duct system. The authors report on a case of annular pancreas characterized by pain and relapsing acute pancreatitis secondary to the malformation. Attention is focused on both the importance of endoscopic retrograde cholangiopancreatography for diagnosing the congenital duct anomaly and the exceptional association of acute pancreatitis and an annular pancreas.

Acute Disease↗
PubMed1984

Vagal influence on exocrine pancreas of alcohol-fed and of normal dogs.

A histochemical study has indicated increased activity of acetylcholine in the pancreas of chronic alcoholic dogs, and we have recently reported decrease pancreatic responsiveness to cholinergic stimulation in such dogs. This prompted us to determine, in chronic alcoholic dogs, the net pancreatic response to stimulation mediated by cholinergic nerves. Therefore, the pancreatic response to vagal stimulation by intravenous 2-deoxy-d-glucose (2DG) infusion was examined in such dogs and in controls. After 2DG, 100 mg kg-1, significant and similar increases in protein output up to maximally 2.7 +/- 0.8 and 2.3 +/- 0.5 mg kg-1 (15 min)-1 were observed in control and alcohol-treated dogs. A significant rise in flow rate and HCO-3 output up to maximally 0.26 +/- 0.07 ml kg-1 (15 min)-1 and 43 +/- 13 mumol kg-1 (15 min)-1, respectively, occurred in the controls but was delayed in the alcoholics. The finding in alcoholic dogs of no change in protein response to 2DG is not in favour of a primary increase of vagally mediated pancreatic protein secretion. It could, however, be compatible with a primary increase in cholinergic receptor resistance due to alcohol and secondary adaptive increase in cholinergic activities, which when combined, would yield no net change of the cholinergically mediated protein response.

Acetylcholine↗
PubMed1984

Modifications in pancreatic enzyme proportions following secretory stimulations.

The short-term influence of repeated secretory stimulations of the pancreas on pancreatic enzyme content was studied in the rat. The animals received 10 successive injections of either cholecystokinin-pancreozymin (CCK-PZ), secretin, CCK-PZ plus secretin, caerulein or pilocarpine. The pancreatic enzyme content was determined the next morning. CCK-PZ, with or without secretin, caerulein and pilocarpine had a similar influence increasing the chymotrypsinogen concentration in the pancreas twofold, while lipase and amylase concentrations increased by only 50 and 25%, respectively. Fasted and fed animals responded similarly. Secretin, a mostly ductal secretagogue, was without influence on the pancreatic enzyme composition. Thus, the mere stimulation of pancreatic protein secretion seems to result in a rapid change in enzyme composition in the pancreas.

Amylases↗
PubMed1984

Immunolocalization of collagen types, laminin and fibronectin in the normal human pancreas.

In order to define the connective matrix organization of the normal human pancreas collagen types I, III, pro-III and IV, laminin and fibronectin were labeled using specific, antihuman antibodies. Visualization was by indirect immunofluorescence. Collagen types I, III and pro-III were present within lobules: around acini, ducts and small vessels. Their immunofluorescence reaction was particularly obvious in septa and it also outlined interlobular vessels and ducts. The type III and pro-III fractions possessed a characteristic, branched appearance in many situations, when compared to the more linear type I reaction. Collagen type IV, laminin and fibronectin were closely applied to acini, ducts and vessels, but in contrast to the other collagen types were absent from septa.

Antibodies↗
PubMed1984

[Value of echotomography in cancer of the pancreas].

We studied by ultrasound (U.S.) a diagnostic method, 87 cases with probable pancreatic cancer; 29 were confirmed surgically. We found an accurate diagnosis in 93.1% of the cases with 6.89% of false negatives and 19.54% of false positives. Likewise, 2.29% of the patients could not be explored by U.S. because they had important meteorism. Among the false positive cases the most important differential diagnosis for us was Chronic Pancreatitis. We conclude that U.S. is a certainly, high sensitive, specific, non invasive and not expensive method. Hence, for us, U.S. is one of the first diagnostic method that must be used when pancreatic neoplasm is suspected.

Adult↗