Search PubMed⌕ Search

Biomedical subjects

H Sanada

Publications and source records attributed to H Sanada.

At least 91 records · Page 5Linked to original sources
PubMed1989

[Chemotherapy of chronic myelogenous leukemia--VP(M) regimen initiated during its chronic phase, and evaluation of MCNU in the phase of blastic crisis].

Seventy-four patients in the chronic phase of Ph1-positive chronic myelogenous leukemia (CML) have been treated with busulfan or other alkylating agents in a conventional way. During its chronic phase, 24 of these 74 cases had received additional intermittent therapy every 4 to 6 months, consisting of vincristine 2 mg or vindesine 3 mg per week, prednisolone 20-30 mg per day and partly 6 mercaptopurine 50 to 100 mg, combined with allopurinol 200 to 300 mg per day for 2 to 3 weeks. The 50% survival of these patients using the Kaplan-Meier's method was 73.7 months and 5-year survival was 69.6%, against 40.5 months and 14.4%, respectively, in the remaining patients. Nine patients in the blastic or accelerated phase of Ph1-positive CML have been treated with new regimens including MCNU. All cases had been refractory for usual types of induction chemotherapy. The new regimen consisted of MCNU 50-100 mg, combined with vindesine or 6-MP plus allopurinol or prednisolone. Five out of 9 cases attained complete remission and 1 partial remission. The major adverse effect of this regimen was slight liver damage. MCNU could be regarded as an useful agent in the blastic phase as well as in the chronic phase of CML.

Adolescent↗
PubMed1987

A unique T-cell line derived from an HTLV-1-negative adult T-cell leukemia patient.

A unique T-cell line, designated ATL-5T, was established from lymphoma cells in pericardial effusion of an adult T-cell leukemia (ATL) patient not carrying HTLV-1 provirus. The cell line is OKT4 and/or Leu3a+ and OKT8 and/or Leu2a+, but interleukin 2 receptor (IL2R)- and HTLV-1 provirus genome negative, and has cytogenetically abnormal karyotypes. The cell line contains rearranged T-cell receptor beta-chain gene, which was identical in rearrangement pattern to the T-cell receptor beta-chain gene in primary cells. These results suggest that factors other than HTLV-1 may sometimes be associated with HTLV-1-negative ATL. The ATL-5T cell line we describe here is unique, and should contribute to further elucidation of the mechanisms involved in the pathogenesis of HTLV-1-negative ATL and HTLV-1-positive ATL.

DNA, Viral↗
PubMed1985

Suppressive effect of dietary unsaturated fatty acids on alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase, a key enzyme of tryptophan-niacin metabolism in rat liver.

The effect of dietary fat on the activity of rat liver alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase [EC 4.1.1.45] (ACMSD), a key enzyme of tryptophan-niacin metabolism, was investigated. When high-fat diet was given to rats for a week, the activity of ACMSD in the liver was extremely suppressed, but not in the kidney. The liver enzyme activity was correspondingly and constantly reduced half by increasing soybean oil in the diet in 4% stages. The potency of suppression of the enzyme activity in liver was found to be different between soybean oil and lard in diets. Among various dietary fatty acids, saturated fatty acid was observed to be less effective. Unsaturated fatty acids, however, were shown to be suppressive and polyunsaturated fatty acid such as linoleic and linolenic acids more effective than oleic acid in suppressing liver ACMSD activity. These suppressive effects of dietary unsaturated fatty acids on the liver ACMSD activity were not considered to be caused by their direct effect on the enzyme protein. Moreover, neither inhibitor nor activator was thought to be involved in the alteration of the enzyme activity.

Animals↗
PubMed1984

Effect of high-protein diet on liver alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase in rats.

In tryptophan-niacin metabolism, alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD) [EC 4.1.1.45] is known to play an important role by catalyzing the decarboxylation of alpha-amino-beta-carboxymuconate-epsilon-semialdehyde, a metabolite of tryptophan which cyclizes spontaneously to form quinolinate. In this study, we investigated the effect of either dietary protein or amino acids on the activity of ACMSD in rats. When rats were fed on a high-protein diet ad libitum for several days, their liver ACMSD activity was greatly increased. Kidney ACMSD activity was also slightly elevated by a high-protein diet. Tryptophan, a precursor of the substrate of ACMSD, was not responsible for the increase of ACMSD activity caused by high-protein diet. On the contrary, insufficiency of dietary tryptophan rather elevated ACMSD activity. The increased amount of amino acids other than tryptophan in the diet resulted in the elevation of liver and kidney ACMSD activities. Although the increase of essential amino acids in the diet slightly elevated the liver ACMSD activity, the enzyme activity was enhanced markedly by feeding the diet containing both essential and nonessential amino acids in high concentrations. These results indicate that at least several amino acids, including some essential and nonessential ones, are responsible for the elevation of ACMSD activity which is induced by high-protein diet. Since liver ACMSD activity in adrenalectomized rats was also increased by feeding a high-protein diet, adrenocortical hormone was thought to be unnecessary for such enzyme induction.

Amino Acids↗
PubMed1983

Adult T-cell leukemia. Chromosome analysis of 15 cases.

Chromosome studies was conducted on 15 patients with adult T-cell leukemia. Cells with chromosomal abnormality were seen in 14 of the 15 patients. The modal chromosome number was near diploid range in all the patients. The most common abnormality was 14q+ marker chromosome and partial deletion of the long arm of chromosome 6, i.e., 6q-, which were seen in eight and seven cases, respectively. Donor chromosomes involved in the 14q+ marker chromosome varies, i.e., Yq, #5p, #5q, #9q, #10q or #12q, except for two patients whose donor chromosome origins were unable to determine. The break point in 14q+ marker chromosome was band at q32. The 6q- chromosome was due to a deletion in one patient and interstitial deletion in six patients. A 14q- chromosome having break point at q24 was found in one patient and duplication of Yq chromosome in two patients. In addition, four patients showed a 5q- chromosome or a 9q- chromosome which was due to a translocation or deletion. The significance of these chromosome abnormalities was discussed.

Adult↗
PubMed1983

Superoxide anion (O2-) production by neutrophils in refractory anemia with excess of blasts.

The O2- production by neutrophils was examined in 4 cases of refractory anemia with excess of blasts (RAEB) in order to evaluate the possible causes of enhanced susceptibility to infection and to gain some informations on the differentiation of neutrophils in this hematological disorder. In three of the four RAEB cases there was little O2- production by neutrophils, in addition to there being morphological anomalies of the neutrophils such as a Pelger-Hüet-like anomaly, granular deficiency and binucleated cells. These results suggest that the impairment of O2- production by neutrophils in RAEB is one of the possible causes of susceptibility to infection and also suggest that the differentiation of neutrophils in this hematological disorder is faulty. The estimation of O2- production by neutrophils may be a useful diagnostic method for preleukemia.

Aged↗