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Biomedical subjects

H Sakio

Publications and source records attributed to H Sakio.

At least 37 records · Page 2Linked to original sources
PubMed1989

Effects of dibutyryl cyclic AMP on hemodynamics and plasma catecholamine concentrations during ammonium chloride-induced metabolic acidosis in anesthetized dogs.

We investigated, using anesthetized dogs, the effect of dibutyryl cyclic AMP (db-cAMP), a derivative of cyclic AMP (cAMP), on cardiovascular variants and plasma catecholamines during metabolic acidosis. These effects were also compared with those of dopamine. The db-cAMP and dopamine were infused at 200 and 20 micrograms/kg.min, respectively. Metabolic acidosis (pH 7.00, PaCO2 40 torr) was induced by the iv infusion of 1-M ammonium chloride solution (NH4Cl). In the normal acid-base state, both db-cAMP and dopamine significantly increased cardiac output and decreased systemic vascular resistance (SVR). During metabolic acidosis, db-cAMP increased cardiac output by 69 +/- 14% and decreased SVR by 36 +/- 2%, while dopamine did not produce changes in cardiac output and increased SVR. Dopamine caused an elevation of epinephrine and norepinephrine in the normal acid-base state, but db-cAMP did not. During metabolic acidosis, dopamine significantly increased the plasma concentration of epinephrine and norepinephrine, while db-cAMP significantly decreased epinephrine concentration. These results suggest that db-cAMP may have a more beneficial effect on hemodynamics compared with dopamine when therapeutic support is needed during circulatory insufficiency with severe metabolic acidosis.

Acidosis↗
PubMed1989

[Transesophageal endoscopic ultrasonography in lung cancer involving mediastinal organs in the assessment of resectability].

The resectability of primary lung cancer depends on the extent of invasion to the mediastinum. To evaluate whether transesophageal endoscopic ultrasonography (E-EUS) could detect mediastinal invasion of lung cancer or not, this method was applied in 22 lung cancer cases in which mediastinal invasion was suspected on the basis of the chest roentgenogram and/or CT scan. An ultrasonic probe with a 5 MHz linear array transducer, 12 mm in diameter and 40 mm in length was attached to the tip of an esophagofiberscope for this study. Ultrasonographic findings were classified into four grades, grade 0; existence of a hyperechoic area between the tumor mass and the mediastinum, grade I; tumor mass in direct contact with mediastinum which appears to be normal in structure accompanied by movement of tumor mass on respiration, grade II; partial disappearance of laminar structures and changes in the mediastinal wall, with no movement of the tumor mass relative to the mediastinum, grade III; tumor invasion within the lumen of the mediastinum. Organs contained by the mediastinum include the pulmonary arteries, aorta, left atrium, pulmonary veins, superior vena cava (SVC) and the esophagus were clearly visualized in detail as real-time images, therefore more detailed information about changes in the laminar structures of walls could be obtained. The sensitivity, specificity and accuracy of E-EUS were 77.8%, 81.8% and 80.0%, respectively. E-EUS could detect mediastinal invasion more accurately than chest roentgenogram, CT or MRI. Particularly, in the SVC, descending aorta and esophagus, the longitudinal extent of tumor can be analyzed accurately. These results suggest that E-EUS can be useful to assess resectability and also to decide the surgical margin preoperatively in case of primary lung cancer in which mediastinal involvement is considered.

Esophagoscopy↗
PubMed1989

[The participation of the sympathetic nervous system in thromboxane A2 release induced by limb ischemia].

Our previous studies indicate that the blockade of the sympathetic nervous system by spinal anesthesia or epidural anesthesia inhibits the release of TXA2 induced by limb ischemia with thigh tourniquet in patients with lower limb surgery. The present study was undertaken to confirm this phenomenon in anesthetized dogs. Twenty one anesthetized dogs underwent 60 min occlusion of the abdominal aorta and the inferior vena cava, followed by reperfusion. They were randomized into three groups: no treatment group and pretreatment groups with either 2 ml of 0.5% bupivacaine intrathecally or 4 mg.kg-1 of labetalol intravenously. In no treatment group plasma TXB2 levels were increased significantly after reperfusion. However, prior treatments with bupivacaine intrathecally or labetalol intravenously inhibited the increases in plasma TXB2 levels after reperfusion. These results suggest that limb ischemia stimulates the TXA2 production, which may be influenced not only by endothelial cell damage and the generation of oxygen free radicals but also by the activation of the sympathetic nervous system.

Animals↗
PubMed1986

[Clinical evaluation of serum NSE and CEA in primary lung cancer patients].

The clinical value of serum neuron specific enolase (NSE) as a tumor marker was evaluated in comparison with simultaneously measured serum CEA, using 74 cases of lung cancers, 13 cases of non-tumorous and non-neurological disease and 28 normal volunteers. The results obtained were as follows: Thirty-five out of the 74 lung cancer cases showed positive serum NSE levels. However, none of the cases of non-tumorous, non-neurological disease and normal volunteers demonstrated more than 10ng/ml NSE which was significantly different from the results of lung cancer cases. We found a higher degree of NSE positiveness in cases of small cell carcinoma than in cases of other histological types. However, no obvious difference of CEA positiveness between any histological types was revealed. Both serum NSE and CEA showed an increase in positive rate in parallel with the progress of stages, including 80.4% of cases of stages III and IV disease which had positive serum levels of both or either of the two markers. However, we could not find any obvious correlation between serum NSE and CEA. The serum levels of both markers clearly decreased after surgery, serum CEA level being considered to be a better reflection of host tumor burden than NSE. In conclusion, the simultaneous testing of serum NSE and CEA is considered to be very useful for evaluating the clinical course or the effect of cancer therapy in lung cancer patients, with higher specificity and sensitivity.

Adenocarcinoma↗
PubMed1983

Effect of acebutolol on plasma catecholamine concentrations in anesthetized dogs with ouabain-induced arrhythmias and its direct actions in vitro on the adrenal medulla.

We have performed studies on blood hormone dynamics following intravenous administration of acebutolol, a newly synthesized beta-blocker, and its direct action on the adrenal medulla in vitro. Intravenous injection of acebutolol into anesthetized dogs almost doubled the plasma adrenaline and noradrenaline concentrations within 5 to 15 minutes, while renin activity was reduced to approximately two-thirds of the pre-administration level. When arrhythmia was induced in dogs with ouabain, the plasma adrenaline and noradrenaline levels increased to 220 +/- 109 and 392 +/- 84 pg/ml, respectively, from the basal levels of 44 +/- 24 and 140 +/- 43 pg/ml. The restoration of sinus rhythm following the administration of acebutolol was accompanied by a further increase in the plasma adrenaline and noradrenaline levels to 797 +/- 364 and 1226 +/- 263 pg/ml, respectively. A perifusion experiment indicated that acebutolol directly accelerated catecholamine release from the adrenal medulla in pigs.

Acebutolol↗