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Biomedical subjects

H S Maker

Publications and source records attributed to H S Maker.

At least 37 records · Page 2Linked to original sources
PubMed1980

Regional distribution of glutathione peroxidase in the adult rat brain.

Glutathione peroxidase activity was measured in 10 areas of perfused adult rat brain with the use of a fluorometric assay coupled to NADPH oxidation. The caudate-putamen and the substantia nigra had the highest activities. Cortical areas and several nuclear areas had somewhat lower activity. Activity was lowest in a white matter structure (corpus callosum). High activity of glutathione peroxidase may be related to the need to reduce hydrogen peroxide arising in the course of monoamine metabolism.

Animals↗
PubMed1979

Effects of anti-white matter serum on myelin and lipid synthesis in brain prisms.

Tissue prisms prepared by choping whole mouse brain maintained respiratory capacity and ultrastructural integrity of 3 h in vitro. Normal rabbit serum (ca. 25%) caused no morphological change but inhibited the synthesis of galactolipids by the prisms. Heating the serum abolished the inhibition. Complement containing anti-white matter rabbit serum destroyed myelin and inhibited galactolipid synthesis to a greater degree than did normal serum. Structures other than myelin were unaffected by the antiserum. Incubation in the presence of heated anti-white matter serum eliminated the myelin destruction but resulted in specific morphological changes characterized by the doubling of the myelin lamellae at the intraperiod line. Immunoperoxidase studies suggest specific binding of immunoglobulin to components of myelin located at the intraperiod lone. These changes were similar to those found in organotypic cultures. Heated antiserum did not inhibit galactolipid synthesis but addition of complement (normal guinea pig serum) to the heated antiserum restored only that portion of the inhibition which exceeded that caused by normal serum. Heat labile factors in normal rabbit serum which inhibit myelin lipid synthesis in the prisms must be corrected for in studies in which the heating of serum is used to demonstrate that the effect is complement dependent. The prism system is simpler than that of organotypic cultures and may be useful in the study of myelinotoxic factors.

Animals↗
PubMed1978

Effect of phospholipids on maintenance of the cytotoxicity of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in aqueous suspension.

Although the chemotherapeutic agent 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) is highly unstable in aqueous suspension, polar glycerophospholipids, notably phosphatidyl choline, had a protective action on the drug's cytotoxicity in aqueous suspension. The cytotoxicity of CCNU against C-6 glioma cells in monolayer culture was maintained longer when the drug was preincubated in the presence of phosphatidyl choline (0.5 mg/ml) than when it was preincubated in medium alone or with serum or when other phospholipids were added. Preservation of drug action may have been due to the formation of common micelles between CCNU and the phospholipid. The importance of the lipid interaction to the effectiveness of the nitrosoureas in vivo is unknown, but such interaction could be a factor in the duration of drug action, its access to the central nervous system, and its attachment to cell membranes.

Cell Survival↗