[Professor Hugo Wilhelm Knipping, M. D., Cologne, on his 70th birthday].
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History, 20th Century↗
Biomedical subjects
H Rink
Publications and source records attributed to H Rink.
Immunological characterization of calf lens gamma-crystallins, separated by preparative isoelectric focusing.
Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological component, the gamma 1b-crystallin corresponds to two polypeptides with molecular weights of 20,500 and 22,000. The two minor components, the gamma 1c- and gamma 1d-crystallin, are immunologically partial identical with the main component gamma 1b-crystallin. One of these minor components, the gamma 1d-crystallin, has a MW of 29,000. The 4th antigen (gamma 1a-crystallin), which has a MW of 21,000, seems to have only remote relationship with the major gamma-crystallin component.
Animals↗
Analysis of synthetic peptides using matrix-assisted laser desorption ionization mass spectrometry.
Matrix-assisted laser desorption ionization mass spectroscopy (LDI MS), a novel method for analysis of large molecules, has been used for characterization of synthetic peptides and their by-products. The potential of LDI MS is demonstrated by analyzing crude synthetic peptides representing typical members of newly designed peptides and proteins. In the first case, a fragment condensation reaction yielding a highly hydrophobic six-helic bundle template-assembled synthetic protein (TASP) is monitored. Then, a crude 19-mer peptide designed to adopt an amphiphilic alpha-helical structure and its by-products from SPPS are identified. Finally, analysis of crude hirulog-1, a 20-mer peptide designed as a thrombin inhibitor, using C18 reversed phase high performance liquid chromatography (RP HPLC), capillary electrophoresis (CE) and LDI MS, manifests the potential of the latter method.
Amino Acid Sequence↗
[Aging of lens epithelial cells and their possible effect on lens opacity (senile cataract)].
Proliferation capacity, synthesis of lens-specific proteins, DNA alterations after UV- and X-ray irradiation as well as their repair capacity have been investigated in tissue cultures with isolated lens epithelial cells of different species and different donor ages, respectively. The in-vitro life span of diploid lens epithelial cells is limited. It is correlated positively with the life expectancy and negatively correlated with the donor age of the investigated animals. The capacity to synthesize lens-specific proteins is lost in late cultures of lens epithelial cells. The capacity to repair UV- and X-ray-induced DNA alterations does not change during aging in vitro, however, it decreases to 75% in cultures derived from old donor animals (bovine). Together with the special growth pattern of the organ lens, the in-vitro results are discussed in order to point out a possible mechanism which might contribute to the development of age-dependent lens opacities. The system "lens epithelial cells in vitro" is considered as a well-suited model system to study age-dependent alterations in the organ lens.
Aged↗
Computer modeling of cytokeratin release in clinical oncology.
The levels of cytokeratins (CK) in serum of cancer patients have been widely used for monitoring progression of cancer growth and the effectiveness of cancer treatment. Previous studies have shown that the release of CK by tumors in patients is a complex process which depends on the rate of cell damage caused by an increasing tumor mass, or by the tumor treatment, but is not in any simple manner correlated to the number of proliferating cells or to the total tumor mass (1). The complexity of the CK-releasing process has been analyzed by a computer model which mimics the progress of tumor growth, allows the introduction of different types of treatment (i.e. irradiation, chemotherapy and surgery), and computes the amount of CK released by the tumor, and the level of CK in blood and blood clearance. The computer model can be used to obtain a better understanding of the interactions of various factors, for scheduling of treatment and CK sampling, and for analyzing the effects of treatment.
Biomarkers, Tumor↗