Biomedical subjects

H Ren

Publications and source records attributed to H Ren.

At least 37 records · Page 2Linked to original sources

PubMed2001

[Recombinant human growth hormone downregulates the apoptosis of HepG(2) cells induced by LPS].

OBJECTIVE: To investigate the effect of LPS on human hepatocytes and study whether recombinant human growth hormone (rhGH) could protect hepatocytes from apoptosis induced by LPS. METHODS: HepG(2) cells were treated with LPS (20 microg/ml) or LPS and rhGH for 16 hours. The apoptosis of HepG(2) cells was detected by terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) or electron microscopy. RESULTS: HepG(2) cells treated with LPS exhibited some specific morphological features of typical apoptosis. the percentage of apoptotic cells in HepG(2) cells treated with LPS and hrGH was significantly lower than that treated with LPS (36+/-5.6)% vs (99+/-0.8)%, P<0.001). CONCLUSIONS: LPS can induce apoptosis of HepG(2) cells, and hrGH can downregulate the apoptotic role of LPS on HepG(2) cells.

Apoptosis↗

PubMed2001

[Humoral immunization and cell-mediated immunization evoked by HBsAg and B7-2 Ag coexpression recombinant adenovirus vector].

OBJECTIVE: To evoke cytotoxic T lymphocytes (CTL) response and seek for a more effective method to treat chronic hepatitis B. METHODS: The adenovirus vector was constructed with the foreign genes inserted in the early region 1(E1), which directed coexpression of HBV-S and B7-2 antigens by means of an internal ribosomal entry site placed between the two coding sequences. The vector was transfected into 293 cell lines by liposome and the adenovirus expressing the target antigens was obtained by plaque select. The HBsAg and B7-2 antigen expression in in vitro cell culture was measured by ELISA and Western blotting, respectively. The immune responses were measured by ELISA for antibody response and a LDH release assay for CTL activity after immunization with the recombinant adenovirus vector in C57 mice. RESULTS: HBsAg and B7-2 antigens were highly expressed after infecting the 293 and HepG2 cell lines in vitro. The humoral response to hepatitis B surface antigen was mildly induced and could be enhanced by reinjecting a regular dose of HBsAg antigen vaccine. The cell-mediated immune response was highly induced by the recombinant adenovirus infection. No clear side effect was observed after immunization. CONCLUSION: This could be a novel strategy for a development of both preventive and therapeutic vaccines against HBV infection. The recombinant adenovirus vector is an effective and safety vector system suitable to the experiments of gene immunization and gene therapy for incurable diseases.

Adenoviridae↗

PubMed2001

[Influence of electroporation on the biological activities of primary rat hepatocytes].

OBJECTIVE: To investigate the influence of electroporation on the biological activities of primary rat hepatocyte and to optimize the electroporation conditions introducing foreign genes into hepatocytes. METHODS: A single-pulse procedure was performed at low voltage (220-400 V) but high capacitance (500-950 microF). Its influence on hepatocyte activities was detected by Trypan blue exclusion (TBE) and MTT analysis. Besides, ALB, ALT and LDH in the supernatants of hepatocytes were tested by biochemical assay. RESULTS: Little hepatocyte damage and high survival rate (>90%) was found from 36 hours till 9th day of culture. At 36th hour after electroporation, ALB, ALT and LDH in the supernatants of Group B (220V, 950 microF) and C (400 V, 950 microF) were higher than those of control group. Whereas TBE and MTT analysis failed to indicate the significant difference of cell viability between electroporation groups and control group. CONCLUSIONS: This electroporation procedure is one of the optimal choices to introduce foreign genes into primary rat hepatocytes.

Animals↗

PubMed2001

[Characteristics of granular sludge during start-up of the internal circulation].

The quick start-up of the laboratory scale IC reactor and the characteristics of granular sludge during start-up were studied in this paper. The results showed that the first start-up of IC reactor could be finished in 20 days, while secondary start-up only needed 15 days with COD loading rate of 12-15 kg.(m3.d)-1 and COD removal larger than 85%. During start-up, the characteristics of granular sludge changed greatly: average granular diameter was increased from 0.88 mm to 1.25 mm; average settling velocity was enhanced from 35.4 m.h-1 to 105.17 m.h-1; methanogenic activities of the granular increased up to 4 times as large as the seeded sludge; the main methanobacteria was changed from Methanothrix to Methanococcus and Methanobacterium.

Sewage↗

PubMed2001

[Construction of a dicistronic expression plasmid vector containing double-valent hepatitis B surface gene].

OBJECTIVE: In order to enhance vaccine response, we constructed a dicistronic expression plasmid containing double HBsAg immunogenes. METHODS: At first, pcDNA3.1 plasmid vector was digested with NheI and EcoRI to get the coding sequence of the small (S) surface protein of HBV, then cloned into pCI-neo vector and named it pCI-S. By PCR amplification, the product of IRES-S was digested with SalI & BamHI, and cloned into pBluescript IIK+S to generate pBKS-IRES-S vector, then subcloned to the pCI-S plasmid to generate pCI-S-IRES-S, which is a dicistronic plasmid of double value HBsAg genes. RESULTS: Two plasmids we constructed were digested with related restriction nucleic enzymes. Sequence analysis of HBsAg and IRES-S gene did not reveal any mutation. CONCLUSIONS: The construction of dicistronic plasmid of divalue HBsAg immunogenes has been well cloned, which is convenient for further research on cell expression and gene immunization in animals.

Antigens, Viral↗

PubMed2001

[Expression of HBsAg with four eukaryotic expression plasmids in immortalized B-cell line from a chronic hepatitis B patient].

OBJECTIVE: To establish efficient eukaryotic expression system of HBsAg in immortalized B-cell line from a chronic hepatitis B patient, as autologous HLA target cells for detecting HBsAg-specific cytotoxic T-lymphocyte (CTL) response. METHODS: Four eukaryotic expression plasmids inserted HBsAg gene were transfected into immortalized B-cell line from a chronic hepatitis B patient, then cells were selected with G418 or hygromycin B. HBsAg in culture supernatants and cell lysates were detected with enzyme-linked immunosorbent assay (ELISA). RESULTS: All eukaryotic expression vectors-transfected immortalized B-cells produced HBsAg, which was readily detectable in culture supernatants and cell lysates. CONCLUSIONS: Transfection of EBV-immortalized B-cell line with four eukaryotic expression plasmids leads to stable expression of HBsAg, which can be used as autologous HLA target cells for detecting HBsAg-specific CTL response.

Adult↗

PubMed2001

Construction and identification of a single stranded cDNA clone containing full-length genome of hepatitis G virus.

AIM: To construct a single cDNA clone with full-length genome of hepatitis G virus (HGV) could be transcribed and expressed in vitro. METHODS: The 5 initial HGV cDNA fragments of Iw5, Iwq2, Iwh6, Iw3 and Iw3 used in this study were amplified from serum of a Japanese non A-E hepatitis patient. These fragments overlapped and covered the entire genome from 5'-end to 3'-end of HGV cDNA. Overlap extension PCR and ligation methods were used with 12 primers for the construction of a full-length genomic HGV cDNA clone from the subgenomic fragments. RESULTS: A single HGV cDNA clone (pHGVqz) was successfully constructed, physical mapping of the generated pHGVqz found identical to what we expected, and the sequence was deposited with the GenBank under the Accession number AF081782. The analysis of the full-length sequence, which was able to be in vitro transcribed and expressed, showed that this single clone contained 9373 nucleotides (encoding 2873 amino acids), and shared high homologies with other compared HGV isolates. CONCLUSION: A full-length genomic HGV cDNA clone is generated for the first of the kind in this study, it could be expressed and transcripted. This single cDNA clone is expected to be of importance in the investigation on replication and pathogenicity of HGV.

Blotting, Western↗

PubMed2001

[Cytomegalovirus enteritis in recipients of allogeneic peripheral blood stem cell transplantation].

OBJECTIVE: To report 6 cases of cytomeganlovirus (CMV) enteritis in allogeneic bone marrow transplantation (allo-BMT) or peripheral blood stem cell transplantation(PBSCT) recipients and the outcome after treatment. METHODS: The 6 patients suffered from leukemia and received allo-BMT or allo-PBSCT. RESULTS: Five of the 6 patients had acute GVHD II-III at 42, 26, 66, 45 and 57 days after transplantation respectively and they recovered after proper treatment. However they soon had severe diarrhea, abdominal pain or/and gastrointestinal bleeding, 5 of the 6 patients received endoscopic examination with biopsy at 50, 57, 80, 65, 35 days after transplantation respectively. They were all diagnosed as having CMV enteritis based on the presence of cytomegalic cells on mucosal biopsy specimens stained with hematoxylin and eosin. Meanwhile the immunoperoxidase stain of histologic specimens for CMV antigen or in situ hybridization by CMV DNA probe was positive. One patient was diagnosed CMV enteritis at 138 days after transplantation based on the clinic and response to therapy. They received antiviral treatment with ganciclovir (DHPG) 500 mg/d for 4 to 21 days, foscarnet 2.4 g q8 h or 4.8 g q12 h for 21 to 90 days, garlic extract 60-120 mg/d, globulin and other supportive measures. All the 6 cases had complete clinical response. CONCLUSION: CMV enteritis should be diagnosed as soon as possible with histopathologic examination and early proper treatment may lead to good clinical response.

Adult↗

PubMed2001

[Clinicopathological analysis of 6 cases of diffuse panbronchiolitis].

OBJECTIVE: To study the clinical and pathological characteristics of diffuse panbronchiolitis (DPB). METHODS: Clinical history of six cases of DPB and their pathological slides were studied and analyzed. RESULTS: Diffuse panbronchiolitis was characterized by chronic recurrent nasosinusitis and pulmonary infection and respiratory bronchiolitis. Pathologically, all layers of the respiratory bronchiole walls were involved. Prominent chronic inflammation with lymphocytes, plasma cells and histiocytes infiltration was noted in all cases. In the lumens of the bronchioles, PMN or mucus could be identified. The chronic inflammatory cell infiltration also contained numerous foamy macrophages within the walls of the small bronchioles and in the stroma of the lung as well. Among the 6 cases of DPB, two cases were associated with thymoma. CONCLUSIONS: DPB is a distinct clinicopathological entity. Open lung biopsy or thorocoscopic biopsy is necessary for the correct diagnosis of clinical atypical DPB cases.

Adolescent↗

PubMed2001

Urinary excretion of aquaporin-2 water channel protein in chronic heart failure rats.

OBJECTIVE: To study the urinary excretion of aquaporin-2 (AQP2) water channel protein, and the relationship between urine AQP2 concentration and renal AQP2 gene expression in chronic heart failure (CHF) rats. METHODS: Male Sprague-Dawley rats (200 g-250 g) underwent either a left coronary artery ligation, a model of CHF, or a sham-operation. Nine weeks after surgery, urinary AQP2 concentrations and renal AQP2 protein levels were measured by Western blot. RESULTS: The urinary concentration of AQP2 water channel protein increased significantly in CHF rats as compared with sham-operated rats (365.6% +/- 102.9% vs 98.5% +/- 47.6%, P < 0.01). There was positive correlation between urinary AQP2 concentration and renal AQP2 protein expression (r = 0.89, P < 0.01). CONCLUSION: The urinary concentration of AQP2 water channel protein increases significantly in chronic heart failure rats.

Animals↗

PubMed2001

Clinical, brain electric earth map, endothelin and transcranial ultrasonic Doppler findings after hyperbaric oxygen treatment for severe brain injury.

OBJECTIVE: To analyze the effect and mechanism of hyperbaric oxygen (HBO) treatment for severe brain injury (SBI). METHODS: Fifty-five patients were divided into a treatment group of 35 patients and a control group of 20 patients. We observed the alterations of clinical, brain electric earth map (BEAM), endothelin (ET) and transcranial ultrasonic Doppler (TCD) findings before and after HBO treatment as well as outcome. RESULTS: In the treatment group, Glasgow coma scale, BEAM and outcome improved after HBO treatment; compared with that of the control group, it showed a significant difference. After one course of treatment, treatment group ET was reduced from 91.24 +/- 12.18 ng/L to 68.88 +/- 14.37 ng/L (P < 0.01); in control group, ET was reduced from 90.78 +/- 15.71 ng/L to 83.12 +/- 12.22 ng/L, with a statistically significant difference (P < 0.05). TCD records of MCA mean velocity (Vm) was reduced from 64.2 +/- 4.8 cm/s to 51.6 +/- 4.2 cm/s (P < 0.01), and a decrease in MCA systolic velocity (Vs) and pulse index (PI) values was statistically significant (P < 0.01). CONCLUSION: HBO treatment can improve the clinical, BEAM and outcome of severely brain injured patients, by decreasing acute stage ET and improving the blood velocity of MCA and decreasing cerebral vascular resistance. HBO treatment can reduce cerebral vascular spasms, cerebral ischemia and hypoxia. One of the important mechanisms of HBO treatment for severe brain injury is the lowering of intracranial pressure.

Adult↗

PubMed2001

[Role of tumor necrosis factor receptor 1 and Fas antigen in hepatocellular apoptosis of viral hepatitis B patients].

OBJECTIVE: To evaluate the role of tumor necrosis factor receptor 1 (TNFR1) and Fas antigen in hepatocellular apoptosis and necrosis in viral hepatitis B. METHODS: Seventy paraffin sections from patients with HBV infection were studied. Five cases of normal liver tissues were studied as control. Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). TNFR1 and Fas antigen were determined by immunohistochemistry. RESULTS: Hepatocellular apoptosis and the expression of TNFR 1 and Fas antigen on hepatocytes were not detected in normal liver tissues. In contrast, there was a strong reaction in TUNEL assay and TNFR1 or Fas antigen expressed on hepatocytes in liver tissues infected with HBV. The degree of apoptosis and the expression of TNFR 1 or Fas antigen on hepatocytes had distinct difference in different hepatitis (P<0.005). There was a positive correlation between degrees of the expression of Fas and hepatic apoptosis (P<0.005), but the similar result was not observed in the expression of TNFR 1 and hepatocellular apoptosis. Our observations also showed that TNFR1 was detected in cytoplasm and cell membrane similarly, and Fas antigen was mainly detected in cytoplasm. There was not positive correlation between degrees of the expression of TNFR1 and Fas antigen on hepatocytes. It was showed that 60.9% (28/46) hepatocytes with strong reaction in TUNEL assay expressed both TNFR1 and Fas antigen. CONCLUSIONS: The degrees of hepatocellular apoptosis and necrosis induced by Fas antigen are stronger than that induced by TNF. The expression of Fas and TNFR 1 on hepatocytes simultaneously may enhance hepatocellular apoptosis in hepatitis B patients.

Apoptosis↗

PubMed2001

[Clinical and pathologic analysis of Sjögren's syndrome with renal impairment: a report of 84 cases].

OBJECTIVE: To further study the renal damage of Sjögren's syndrome (SS) and its clinical and pathologic characteristics with biopsy and recent technology. METHODS: 84 patients of SS with renal impairment from 1993 to 1999 were analyzed by routine, immunoassay, tubular function and biopsy examination. RESULTS: 55 of the 84 cases presented with renal tubular acidosis (RTA), 5 with diabetes insipidus and 3 with hypokalemic paralysis. Glomerulopathy occurred in 22 cases (nephrotic syndrome 12, glomerulonephritis 10) and mild renal failure (RF) was found in 14. 69.1% of the patients had hypergamma-globulinemia. 64.3% and 44.1% of the patients showed positive anti SS-A and anti SS-B. In 37 renal biopsy specimens 21 showed chronic interstitial nephritis (CIN) with extensive lymphoplasmic cell infiltration and tubular atrophy. 10 of the 37 specimens revealed lupus nephritis (LN, type III and IV) and 5 mesangial proliferative glomerulonephritis (MsPGN). With immunofluorescence tests, no positive findings were seen in most of the specimens, but deposition of IgA, IgM or C(3) was seen in some patients. IgG deposits in the interstitial lymphoplasmic cells were found in 1 patient. 25 patients were treated with prednisone combined with cytoxan (CTX). In 14 patients with renal failure, serum creatinine level returned to normal after treatment. CONCLUSION: Renal impairment may be the presenting or predominant feature in SS. The major clinical manifestations are RTA and GN. Treatment with prednisone may decrease the infiltration of lymphoplasmic cells in the interstitium, improve the renal function and correct RTA.

Adolescent↗

PubMed2001

Glasgow Coma Scale, brain electric activity mapping and Glasgow Outcome Scale after hyperbaric oxygen treatment of severe brain injury.

OBJECTIVE: To study the effect of hyperbaric oxy gen (HBO) treatment of severe brain injury. METHODS: Fifty-five patients were divided into a treatment group (n=35 receiving HBO therapy) and a control group (n=20 receiving dehydrating, cortical steroid and antibiotic therapy) to observe the alteration of clinic GCS (Glasgow Coma Scale), brain electric activity mapping (BEAM), prognosis and GOS (Glasgow Outcome Scale) before a nd after hyperbaric oxygen treatment. RESULTS: In the treatment group GCS, BEAM and GOS were improved obviously after 3 courses of treatment, GCS increased from 5.1 to 14.6 (P<0.01-0.001),the BEAM abnormal rate reduced from 94.3% to 38% (P<0.01-0.001), the GOS good-mild disability rate was 83.7%, and the middle-severe disability rate was 26.3% compared with the control group. There was a statistic significant difference between the two groups (P<0.01-0.001). CONCLUSIONS: Hyperbaric oxygen treatment could improve obviously GCS, BEAM and GOS of severe brain injury patients, and effectively reduce the mortality and morbidity. It is an effective method to treat severe brain injury.

Adult↗

PubMed2001

[Inhibition of proliferation and induction of apoptosis by simvastatin in K562 leukemic cell line].

OBJECTIVE: To investigate the anti-apoptotic mechanism and explore approach to inhibiting proliferation and inducing apoptosis of chronic myclogenous leukemia (CML) cells. METHODS: K562 cell line was used to evaluate the effects of simvastatin, an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, and the combination of simvastatin with chemotherapeutic agents on the proliferation and apoptosis of CML cells. RESULTS: Simvastatin could significantly inhibit proliferation and induce apoptosis of K562 cells, and could increase the sensitivity of K562 cells to chemotherapeutic agents. Addition of mevalonate, the immediate product of HMG-CoA, could completely reverse this effect. CONCLUSION: Simvastatin inhibited proliferation and induced apoptosis of K562 cells through inhibiting the metabolic pathway of mevalonate. It is promising that HMG-CoA reductase inhibitors may be an effective chemotherapeutic approach to the treatment of CML.

Antineoplastic Agents↗

PubMed2001

[Amphotericin B for treatment of fungal infections in 40 patients with malignant hematologic diseases].

OBJECTIVE: To observe the therapeutic effect and side effects of amphotericin B for fungal infections in patients with malignant hematologic diseases. METHODS: 40 patients (male 27, female 13; average age 35.5 years) with malignant hematologic diseases were given amphotericin B, 5-50 mg/d per day for 5-85 days (average time 21 days). RESULTS: The clinical efficacy rate of amphotericin B was 52.5%, and the fungal elimination rate was 56.2%. Among the side effects, rigor and fever were present in 2.5% of the patients. Hypokalaemia was found in 12.5%, hepatotoxicity in 15.0% and nephrotoxicity in 15.0%. CONCLUSION: As amphotericin B has a broad anti-fungal spectrum and relatively good efficacy, it is still a high-efficiency drug in treatment of systematical fungal infections. However, the use of drug is limited because of its many side effects. Our study indicates that if it is used properly and hepatic and renal function tests are carried out regularly, amphotericin B is a relatively safe and effective drug.

Adolescent↗

PubMed2001

[Cloning and phylogenetic analysis of hepatitis-associated TT virus subgenome].

Polymerase chain reaction (PCR) was utilized for the DNA amplification from transfusion transmitted virus (TTV) positive serum samples. Five TTV DNA fragments, overlapped about 90% of the genome, were amplified by long template PCR for the generation of TTV subgenome. Recombinant plasmids were obtained by directly inserting PCR products into pT-Adv vector, and DNA sequence analyses showed they were TTV DNA fragments. By using specific restriction enzymes, five TTV DNA fragments were ligated into a TTV DNA subgenome clone and named as TTV021. TTV021 has been deposited in GenBank database with the accession number AF254410. The results of computer analyses showed that TTV021, 3472 nt long, contains two open reading frames (ORF1, 785 aa; ORF2, 146 aa). Identity alignments between TTV021 and other TTV isolates indicated several high conserved regions existed. Phylogenetic analysis of 356 nt from TTV021 suggested that the isolate has close evolutionary relationship with CHN1 (type 1a), but has far relation with other TTV isolates.

Circoviridae↗

PubMed2001

[Successful engraftment of HLA-identical sibling cord blood transplantation in an adult with chronic myelogenous leukemia].

OBJECTIVE: To explore the feasibility of cord blood transplantation (CBT) for the treatment of adult hematological malignancies and its long-term hematopoiesis reconstitution and transplantation-related complications. METHODS: An 18 years old patient (body weight 75 kg) with chronic myelogenous leukemia in first chronic phase received HLA-identical sibling CBT after conditioning with modified busulfan/cyclophosphamide (Bu/CTX) regimen. The transplanted number of nucleated cells was 1.73 x 10(7)/kg of body weight, and that of CD34+ cells 2. 7 x 10(5)/kg. Cyclosporin A and methylprednisolone were given as prophylaxis against graft versus-host disease (GVHD). RESULTS: The neutrophil count rose to above 0.5 x 10(9)/L on day 18 and platelet count exceeded 50 x 10(9)/L on day 36. Gene analysis showed that bone marrow cells had completely changed to donor's type on day 80. The patient was diagnosed with grade IV acute hepatic GVHD complicated with CMV infection because of severe jaundice on day 90. After the administration of additional immunosuppressive agents, antiviral agents, plasma exchange and in vitro billirubin adsorption, the complications were well controlled. In the follow-up of 24 months', the patient's general condition is good without obvious hepatic dysfunction and Ph chromosome and bcr/abl fusion gene of bone marrow cells were persistently negative. CONCLUSION: It is the first case reported in China that adult patient with leukemia has been successfully treated by allogeneic CBT, and this indicates that CBT is feasible in the treatment of adult patient with leukemia.

Adolescent↗