[Diagnostic serology of campylobacter infections--advisability of a supplementary review of diarrhea].
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Antigens, Bacterial↗
Biomedical subjects
H Rautelin
Publications and source records attributed to H Rautelin.
Acid and alkaline Campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.
Acid and alkaline extracts from several Campylobacter strains were used as antigens in enzyme immunoassay (EIA). Antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different Campylobacter subspecies. For the preparation of the extracts, three Campylobacter fetus subsp. venerealis strains, three Campylobacter fetus subsp. fetus strains, four Campylobacter coli, and five Campylobacter jejuni strains were used. The acid extracts of Campylobacter fetus subsp. fetus and Campylobacter fetus subsp. venerealis were antigenically similar to each other but different from Campylobacter coli and Campylobacter jejuni strains. These acid extracts were found to be suitable antigens in enzyme immunoassay. However, when alkaline extracts were used as the antigen, fewer antisera reacted with them than with the acid extracts, and many pre-immune sera gave high values.
Animals↗
Single and multiple acid extract antigens in Campylobacter serology.
Campylobacter IgA, IgM and IgG antibody titers of 442 patients with positive isolations of Campylobacter were tested in enzyme immunoassay (EIA). Of the patients, 330 showed elevated antibody end-point titers to a single acid extract antigen prepared from a C. jejuni strain. Sixteen further patients had fourfold or greater antibody titer changes in paired sera. Nine patients, who did not show elevated antibody titers for the single extract, had positive serological responses for a multiple acid extract prepared from six C. jejuni/coli and a C. fetus subsp. fetus strain. The specificity of the test, based on Campylobacter antibodies of 200 healthy blood donors, was 99%. The use of the single acid extract gave a sensitivity of 78%. The additional use of the multiple acid extract raised the sensitivity to 80%.
Antibodies, Bacterial↗
[Campylobacter epidemic in Virrat in the summer of 1985].
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Adolescent↗
An acid extract as a common antigen in Campylobacter coli and Campylobacter jejuni strains.
An acid extract prepared from a single strain of Campylobacter jejuni reacted with rabbit antisera against 21 strains of C. jejuni and Campylobacter coli. The immunogen is resistant to Formalin and to exposure to 100 degrees C for 30 min. The antigenic extract may have practical applications in human serology.
Acids↗
Antibodies against an acid extract from a single campylobacter strain in hospitalized campylobacter patients.
An acid glycine extract from a single Campylobacter jejuni strain was used as the antigen in the enzyme immunoassay (EIA). Elevated values indicating the presence of specific IgM and/or IgG antibodies were found in 73% of 52 patients hospitalized for campylobacter infection. A further 6% of the patients who had lower EIA values in paired serum samples showed at least a 20% change in EIA values. The same sera had been studied earlier for agglutinating antibodies using four reference campylobacter strains. 74% of the patients had shown elevated titres and a further 17% four-fold changes of lower titres in paired sera.
Antibodies, Bacterial↗