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Biomedical subjects

H R Morgan

Publications and source records attributed to H R Morgan.

6 recordsLinked to original sources
PubMed1979

A simplified retropubic urethropexy in the treatment of primary and recurrent urinary stress incontinence in the female.

Urinary incontinence presents a severe social handicap to the patient and a challenge which has taxed the imagination and ingenuity of surgeons for over a century. Restoration of the urethrovesical junction to its normal retropubic intra-abdominal level will usually result in the cure of pure anatomic female stress incontinence. A retropubic approach is utilized in order to provide for identification and mobilization of the urethra, bladder neck, and vagina. Nonabsorbable sutures are used to attach the perivaginal fascia to the pectineal ligament. The operative technique is described in detail. A series of 77 patients who have been followed for up to 3 1/2 years is presented, showing a stress incontinence cure rate of 96.1%.

Adult↗
PubMed1977

A problem-oriented independent studies programme in basic medical sciences.

An independent studies programme (ISP) in the basic medical sciences has been developed at the University of Rochester School of Medicine and Dentistry which involves sixteen students as a learning team working with ten medical teachers as a teaching team. The teachers serve as guides to learning rather than as sources of expert information and function as generalists in their educational roles. The programme, which is preferred by some students, has provided an alternative to the lecture-laboratory-conference format of the traditional curriculum. Some of the advantages of such an ISP are discussed and its effectiveness assessed.

Curriculum↗
PubMed1976

Neutral protease activity of Rous sarcoma (RSV) transformed chick embryo fibroblasts.

The proteolytic activities of normal, Schmidt-Ruppin Rous sarcoma virus (SR-RSV) transformed, and infected (RAV) chick embryo fibroblasts (CEF) have been measured by a highly sensitive technique using 3H-acetylated haemoglobin as a substrate. When all 3 types of CEF cells were maintained in serumless media, no differences were detected in the amount of pH 3-4 protease activity released into the media over a 24-h period, and only negligible amounts of pH 7-6 proteolytic activity were found. When normal, transformed, and infected cells were maintained in serumless media and later incubated with 3H-acetylate haemoglobin, a significant proteolysis of the haemoglobin, a 6-fold increase compared to the normal CEF cells, was associated only with plates containing SR-RSV-CEF cells. A fluorescent assay for peptides confirmed that SR-RSV-CEF cells have increased cell-associated proteolytic activity. The net surface charge of the transformed CEF cells was unchanged by maintenance in serumless media but the net surface negativity of the normal and RAV-CEF cells was significantly increased by incubation in media minus serum for 24 h. This suggests that normal CEF cells, maintained in media plus serum, have a substance masking their surface charge which is absent from the surface of transformed cells, possibly because of proteolytic degradation.

Avian Leukosis Virus↗
PubMed1975

Cell surface changes accompanying viral transformation: N-acetylneuraminic acid ectotransferase system activity.

The activity of the sialyl ectotransferase system of normal chick embryo fibroblasts (CEF) and chick embryo fibroblasts transformed with the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV) have been compared. Neuraminidase treatment of the intact cells increased the sialyl ectotransferase system activity of control and transformed cells two to three times. The ectotransferase system activity increased as the pH was decreased from 7.8 to 6.0. The temperature optimum for both systems was 40 degrees C. Approximately 60% of the 14C-sialic acid incorporated at pH 6.5 or above could be removed with neuraminidase. The activity of the transformed cell system with or without neuraminidase treatment was more stimulated by addition of Mn2+ ions, particularly above pH 7.0. This difference in ion sensitivity indicates that a different cell surface phenomenon is being studied after transformation.

Acetyltransferases↗