Production levels of p-coumaric acid (p-CA), p-hydroxyphenylbut-3-ene-2-one (benzalacetone), and p-hydroxyphenyl-2-butanone (raspberry ketone) were measured in raspberry cell suspension cultures to investigate metabolite dynamics in a short (two-step) pathway. Intracellular concentrations of benzalacetone and the raspberry ketone fluctuated during the time course of a normal batch culture cycle but showed higher levels during periods of rapid growth. Cells elicited with the signal coupler methyl jasmonate yielded a 2- to 3-fold increase in metabolite concentrations after 24 h. The results suggest that raspberry ketone production is rapidly inducible during periods of high carbohydrate utilization. It is not an end product, however, and undergoes conversion to subsequent metabolites.

Butanones↗

PubMedDate not supplied

Stable genetic transformation of Eschscholzia californica expressing synthetic green fluorescent proteins.

An efficient protocol is described for the stable genetic transformation of Eschscholzia californica (California poppy) using Agrobacterium tumefaciens as a vector. We have employed the disarmed A. tumefaciens LBA4404 encoding a synthetic green fluorescent protein reporter gene that is further controlled by an enhanced cauliflower mosaic virus 35S promoter. Stably transformed E. californica cells appear 3 weeks after initial cocultivation of A. tumefaciens with poppy leaves, stems, or roots. Transformed poppy calli were visualized by exposure to long-wave UV or blue light and analyzed in detail by fluorescent microscopy and laser-scanning microscopy. Moreover, green fluorescent calli have been maintained through continual subculture and grow well either on Gamborg's B5 agarose or liquid medium.

Agrobacterium tumefaciens↗

PubMedDate not supplied

Clinical trial of the oral form of a new podophyllotoxin derivative, VP-16-213 (NSC-141540), in patients with advanced neoplastic disease.

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Administration, Oral↗

PubMedDate not supplied

Fibrin sealant in experimental tubal microsurgery.

This study compares the results of microsurgical tubal anastomosis performed using a fibrin sealant, 9-0 monofilament polyglycolic acid (PGA) suture, or fibrin sealant in combination with 9-0 PGA suture. Sixty rabbits were divided into three groups of 20 rabbits each. The pregnancy rate of the rabbits was 95%, 95%, and 85%, respectively; without any intervention, the fertility rate was 95% in 20 nonexperimental rabbits (control group). We conclude that fibrin adhesive employed for reanastomosing of the fallopian tube in rabbits, compared with synthetic microsutures, results in identical pregnancy rate and identical conditions of passage through the tubes. Fibrin sealant is absorbed more rapidly than absorbable microsutures, results in fewer adhesions, and shorter operating time. Thus, fibrin adhesive possibly provides an alternative to the traditional employment of sutures in gynecological microsurgery.

Animals↗

PubMedDate not supplied

Mind your backs!

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Back Pain↗

PubMedDate not supplied

Acetylsalicylic acid in the prevention of stroke in patients with reversible cerebral ischemic attacks. A Danish cooperative study.

Two hundred and three patients, 148 males and 55 females, who during the last month before admission had experienced at least one reversible cerebral ischemic attack of less than 72 hours duration, were randomly assigned to treatment with either acetylsalicylic acid (ASA) 1000 mg daily (101 patients) or placebo (102 patients). The average follow-up period was 25 months. The two treatment groups were comparable with respect to age, sex, associated diseases, risk factors, number and duration of cerebral ischemic attacks. No statistically significant differences were found between the treatment groups as to the primary end point: stroke or death (ASA group 20.8%, placebo group 16.7%). Occurrence of transient ischemic attacks during the treatment period was not reduced by ASA treatment, whereas there was a trend suggesting fewer myocardial infarctions in the ASA group (5.9%) than in the placebo group (13.7%). The difference, however, was not statistically significant (p = 0.10). We were thus unable to demonstrate any favorable influence of ASA 1000 mg daily in patients with reversible ischemic attacks. This study does not, of course, prove that ASA treatment is ineffective in stroke prevention.

Adult↗