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Biomedical subjects

H Ono

Publications and source records attributed to H Ono.

At least 559 records · Page 31Linked to original sources

Stereo-deficients and stereoblinds cannot make utrocular discriminations.

In two separate experiments, we show that stereo-deficient observers are no better than stereonormals at discriminating the eye-of-origin of a monocular stimulation. Stereo-deficient observers are considered to have a preponderance of monocular neurons throughout their visual system, including the visual cortex. The results indicate that, in spite of their clear anatomical and physiological structure, such monocular neurons do not convey eye-of-origin information to consciousness.

Depth Perception↗

Utrocular discrimination is not sufficient for utrocular identification.

The distinction between reliability and validity is critical in examining utrocular identification. Four experiments demonstrated that two cues that lead to reliable discrimination do not lead to valid identification. Experiment 1 showed that, in the condition in which a stimulation of the right eye produced a visual direction toward the right and a stimulation of the left eye toward the left, there was a preponderance of correct responses. In the condition in which a stimulation of the right eye produced a visual direction toward the left and the left eye toward the right, there was a preponderance of incorrect responses. Experiment 2 showed that covariation of responses with visual direction decreased when feedback was provided because subjects sought other cues. Experiment 3, which included binocularly deficient subjects, showed that a feeling-in-the-eye is associated with the eye stimulated by a greater change in luminance rather than the eye stimulated by the target stimulus. When the luminance change was greater in the target eye, the feeling led to reliably correct responses, but when the luminance change was greater in the nontarget eye, it led to reliably incorrect responses. Experiment 4 indicated that the proportion of correct responses covaries with the degree of change in the luminance of the nontarget eye. The responses varied from reliably incorrect identifications, through unreliable identifications, to reliably correct identifications. These findings are consistent with the idea that stimulation of either eye is "projected" to the cyclopean eye.

Discrimination, Psychological↗

Extracranial metastasis of glioblastoma with sarcomatous component.

A case of glioblastoma with sarcomatous component is presented. Craniotomy was performed with total resection of the left occipital tumor. The patient received radiotherapy postoperatively but developed extracranial metastases only two months after the first surgical procedure.

Adult↗

Sorbitol determination by liquid chromatography: application to red blood cells of diabetic rats.

This report describes an application of liquid chromatography to the determination of sorbitol in red blood cells. The chromatograph employed in the present study was made up of sub- and main-separation systems and a detector portion. The sub-separation system was for concentration of polyols and involved two small columns, each containing the same anion exchange resin. The first was a tiny column which, in borate form, served as the concentrator of polyols and sugars charged in a large volume, while the second, in acetate form, separated the carbohydrates from the borate. The main system was for the fine separation of each carbohydrate and employed cation exchange columns. The detector part utilized a flow fluorometric method comprising two successive reactions: periodate oxidation followed by the Hantzsch reaction. The resulting whole chromatographic system was applied to the determination of sorbitol in red blood cells obtained from normal rats and rats made diabetic by the administration of streptozotocin; a part of the latter group had also received an aldose reductase inhibitor. Our results supported the concepts that a prolonged duration of high blood glucose level induces an elevated level of sorbitol inside red blood cells and that aldose reductase inhibitors are effective in reducing this level.

Aldehyde Reductase↗

A Fukuyama type of congenital muscular dystrophy associated with atypical gyrate atrophy of the choroid and retina. A case report.

A three-year-ten-month old boy with the Fukuyama type of congenital muscular dystrophy (FCMD), early onset of muscular hypotonia, severe mental retardation, a slow progressive course and a characteristic fundus appearance similar to that of gyrate atrophy is described. Hyperornithinaemia and iminoglycinuria were not noted. To our knowledge, this is the first reported case of FCMD associated with atypical gyrate atrophy of the choroid and retina.

Amino Acids↗

No role for prostaglandins and bradykinin in the autoregulation of renal blood flow.

In order to evaluate the participation of prostaglandins and bradykinin in autoregulation of renal blood flow, the pressure-flow relationships were examined before and during the infusion of prostaglandin E2, sodium arachidonate or bradykinin in the pump-perfused kidney of anesthetized dogs. Renal arterial pressure was changed in a step-wise fashion between 60 and 200 mmHg by means of a pneumatic resistance. Renal blood flow revealed an effective autoregulation against the pressure changes between 100 and 200 mmHg. Infusion of prostaglandin E2 (30 and 100 ng/min), sodium arachidonate (200 and 500 micrograms/min) or bradykinin (100 and 300 ng/min) into the renal artery increased renal blood flow dose-dependently, but had no influence on the autoregulation of renal blood flow. We also examined the effect of endogenous bradykinin and prostaglandins by the intravenous administration of captopril (1 mg/kg), a kininase II inhibitor, and indomethacin (2.5 mg/kg), a cyclo-oxygenase inhibitor, respectively. Captopril increased renal blood flow, but did not impair the autoregulation. Indomethacin decreased renal blood flow and had no effect on the renal autoregulation. It is considered that prostaglandins and bradykinin which induced renal vasodilation have no role in the autoregulation of renal blood flow in the dog kidney.

Animals↗

Computed tomography and chymopapain chemonucleolysis: preliminary findings.

Ten lumbar disk levels in nine patients were examined by computed tomography (CT) before and 6 weeks after chymopapain chemonucleolysis of herniated nucleus pulposus (HNP). Patient selection for chemonucleolysis was partly based on CT scans demonstrating morphologic HNP criteria favoring a successful treatment outcome. Posttreatment CT scans disclosed a measurable HNP size decrease of 1-3 mm at nine of 10 levels studied as well as an increase in HNP attenuation at many levels. CT findings correlated closely with objective clinical parameters used to assess treatment outcomes.

Chymopapain↗

Formadicins, new monocyclic beta-lactam antibiotics of bacterial origin. I. Taxonomy, fermentation and biological activities.

A Gram-negative bacterium produces new monocyclic beta-lactam antibiotics with a formylamino substituent, named formadicins A, B, C and D. The producing bacterium was taxonomically characterized and designated as Flexibacter alginoliquefaciens sp. nov. YK-49. Formadicins have narrow antibacterial spectra. They are highly active against some species of Pseudomonas, Proteus and Alcaligenes. Of the four, formadicin C shows the most potent antibacterial activity. Several amino acids such as glycine, D-alanine and D-leucine were antagonistic against formadicins. Formadicins, especially formadicins A and C having the formylamino substituent bound to the 3-position of a beta-lactam nucleus, were highly resistant to hydrolysis by various types of beta-lactamases. Formadicins A and C showed affinity for penicillin-binding proteins (PBPs) 1A and 1B in Pseudomonas aeruginosa IFO 3080, but formadicin B and nocardicin A showed affinity only for PBP 1B. Formadicins A and C did not lyse Escherichia coli LD-2 solely at their MICs, but when combined with mecillinam each induced a rapid lysis of this organism.

Amino Acids↗

Cephabacin M1-6, new 7-methoxycephem antibiotics of bacterial origin. I. A producing organism, fermentation, biological activities, and mode of action.

New 7-methoxycephem antibiotics were found in culture filtrates of a bacterium isolated from a plant and named cephabacin M1-6. They are the first members of 7-methoxycephem antibiotics of bacterial origin. The producing organism was taxonomically characterized and identified as Xanthomonas lactamgena YK-431; other strains of this species have recently been reported to produce cephabacin F and H group antibiotics. Cephabacin M1-6 exhibited moderate antibacterial activity against Gram-negative and Gram-positive bacteria. Cephabacin M1-6 were as stable as cephamycin C to cephalosporinases. They showed inhibitory activity against a cephalosporinase of Proteus vulgaris GN 4413. The mode of action of cephabacin M1 was examined using Escherichia coli and Bacillus subtilis as test organisms; primary lethal targets of cephabacin M1 are penicillin-binding protein (PBP) 1 in E. coli and PBP 4 in B. subtilis.

Anti-Bacterial Agents↗

Cephabacin M1-6, new 7-methoxycephem antibiotics of bacterial origin. II. Isolation, characterization and structural determination.

Six components of new cephem antibiotics, cephabacin M1-6, were isolated from the culture filtrate of Xanthomonas lactamgena YK-431 by various types of column chromatographies and preparative reverse-phase HPLC. Their structures were determined by spectroscopic analyses and degradation studies. They consist of 7-methoxydeacetylcephalosporin C as a nucleus and a tri- to heptapeptide including a new amino acid, which is bound at the 3-position with an ester bond.

Anti-Bacterial Agents↗

Interactions of formylamino- and methoxy-substituted beta-lactam antibiotics with beta-lactamases.

Cephem and nocardicin-type monocyclic beta-lactam antibiotics with a formylamino substituent were highly resistant to hydrolysis by both penicillinases and cephalosporinases. Among antibiotics with a methoxy substituent, an N-sulfonated monocyclic beta-lactam antibiotic, sulfazecin was resistant to beta-lactamases, but cephem antibiotics were sensitive to the cephalosporinase of Enterobacter cloacae. The resistance of the antibiotics to the beta-lactamases depended primarily on the presence of the substituent, but affinity for the beta-lactamases was affected not only by the substituent but also by the presence of other side chains. Formylamino compounds and sulfazecin were as good inducers of beta-lactamases as semisynthetic 7-methoxycephalosporins, but naturally occurring 7-methoxycephalosporins were poor inducers. The inducer activities of the antibiotics were not necessarily related to their beta-lactamase stabilities. The stabilities of the compounds to the beta-lactamases were well reflected in their antibacterial activities against beta-lactamase producing bacteria.

Anti-Bacterial Agents↗

Alahopcin, a new dipeptide antibiotic produced by Streptomyces albulus subsp. ochragerus subsp. nov.

An actinomycete strain No. B-52653 was found to produce an antibiotic selectively active against the in vitro antibiotic resistant mutant of Staphylococcus aureus. Based on taxonomic studies, the name Streptomyces albulus subsp. ochragerus subsp. nov. is proposed for the strain. The microorganism produced two kinds of antibiotics; one identical with gougerotin, the other an amphoteric water soluble dipeptide containing L-alanine. The latter has the molecular formula C9H15N3O6 and is named alahopcin. It has a broad antibacterial spectrum and a synergistic effect with some other antibiotics against some antibiotic resistant staphylococci. Alahopcin has a low toxicity and was effective against experimental infections in mice caused by Staphylococcus aureus.

Animals↗