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Biomedical subjects

H Okubo

Publications and source records attributed to H Okubo.

At least 127 records · Page 7Linked to original sources
PubMed1978

Distribution of alpha 1-antitrypsin in normal, granuloma, and tumor tissues in rats.

Purified rat alpha 1-antitrypsin and albumin were radiolabeled, and their distribution and catabolism in various tissues were studied in rats with inflammatory granuloma or transplanted sarcoma. Intravenously administered labeled alpha 1-antitrypsin accumulated remarkably in extravascular spaces of the granuloma or sarcoma tissues. Among the normal organs examined, the lung preferentially incorporated alpha 1-antitrypsin. Furthermore, most of the alpha 1-antitrypsin accumulated in these three tissues remained in a TCA-precipitable form throughout the observation period. Since alpha 1-antitrypsin is incorporated in large amounts into inflammatory or tumor tissues, it could play a role in regulation of inflammatory processes or in controlling the proliferation of a tumor. The studies on TCA fractionation also suggest that liver and kidney provide the main sites for degradation of this protein. Although the accumulation of labeled albumin in granuloma and sarcoma was less marked, it showed essentially the same distribution and degradation pattern as alpha 1-antitrypsin in both morbid states.

Animals↗
PubMed1978

Rat alpha 1-acid glycoprotein: uptake by inflammatory and tumour tissues.

The tissue distribution of intravenously administered 125 I-alpha 1-acid glycoprotein and 125I-albumin was studied in rats with inflammatory granuloma or transplanted sarcoma. The marked concentration of alpha 1-acid glycoprotein in extra-vascular spaces of the granuloma or sarcoma tissue was observed, most of which remained in a trichloroacetic-acid-precipitable form throughout the experiments. Albeit less marked, albumin also preferentially accumulated in both pathological tissues and presented essentially the same distribution pattern as alpha 1-acid glycoprotein in intact organs except kidney in both morbid states. The results suggest that serum alpha 1-acid glycoprotein might play an important role in inflammatory or cell proliferative processes in situ. The studies on trichloroacetic acid fractionation also indicate indicat a selective involvement of the kidney in the catabolic processes of alpha 1-acid glycoprotein as compared to that of albumin.

Albumins↗
PubMed1977

Rat alpha1-acid glycoprotein. Purification and immunological estimation of its serum concentration.

A rapid method is described for the preparation of serum alpha1-acid glycoprotein from rats with inflammation induced with turpentine oil injection. The protein obtained by two purification steps, batchwise adsorption with DEAE-cellulose followed by chromatography on CM-cellulose, was proved to be native alpha1-acid glycoprotein in a high degree of purity by electrophoretical, immunological, ultracentrifugal and carbohydrate analysis. The monospecific and potent antiserum to this protein was prepared by immunizing rabbits with the desialyzed material emulsified with Freund's incomplete adjuvant. Using purified alpha1-acid glycoprotein and its specific antiserum, the concentration of alpha1-acid glycoprotein in rat serum was determined by single radial immunodiffusion. Abnormally high levels of its concentration (5-6 times higher than the control) were observed in inflammatory and tumor bearing rats.

Animals↗
PubMed1976

Developmental studies on glucosamine metabolism.

Changes in hepatic hexosamine metabolism and serum seromucoid concentrations during postnatal development were investigated in the rat. A relatively low activity of hepatic L-glutamine: D-fructose-6-phosphate aminotransferase (AT) was observed within 24 hr of birth. This rapidly increased to a maximum at about 2 weeks of age, followed by a decline to adult levels after another 2 weeks. The developmental pattern of hepatic UDP-N-acetylglucosamine 2'-epimerase (EP) closely resembled that of AT. It was relatively low in the newborn, increased to a maximum at about 2 weeks and then declined to adult values after another 2 weeks. The serum seromucoid concentration was low in the new born rats and then increased gradually with development to adult levels at about 50 days. The hepatic concentrations of UDP-GlcNAC and CMP-NANA both were relatively high at 24 hr after birth compared to values observed at 50 days. The developmental pattern observed for both over this time period was quite similar and was almost the reciprocal to that for the seromucoid fraction. After 50 days the concentration of UDP-GlcNAc remained constant at adult levels as did that of the seromucoid fraction, but the concentration of CMP-NANA showed a marked increase. It is suggested that the hepatic concentrations of these amino-sugar nucleotides during development primarily reflect the demand for the hepatic synthesis and secretion of plasma glycoproteins as reflected in the seromucoid fraction.

Aging↗
PubMed1976

[Gentamicin therapy of infectious diseases by pathogens (author's transl)].

Gentamicin (GM) was intramuscularly injected to 29 patients with various infections in the doses of 40-60 mg twice a day. The maximum dose was 1,800 mg. Satisfactory clinical results were obtained in the treatment of not only urinary tract infections but also respiratory tract infections, bile duct infections and others. This clinical results would coincide with the fact that almost all organisms isolated had a high sensitivity to GM. No significant abnormal findings was observed in this clinical study. This clinical result indicates that GM is a useful drug in the treatment of various infections.

Adult↗