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Biomedical subjects

H Ogino

Publications and source records attributed to H Ogino.

At least 217 records · Page 12Linked to original sources
PubMed1983

Synthesis and biological activity of 7 beta-(2-amino-2-carboxy)-ethylthioacetamido-7 alpha-methoxycephalosporin derivatives.

C-7 and C-3 substituents of a new antibiotic SF-1623 were chemically modified to improve the bioactivity, and substituent effect at C-7 and C-3 was examined on the basis of MIC and ED50 values against selected bacteria. Among a large number of derivatives, MT-141 possessing 2-D-2-amino-2-carboxyethylthioacetamido residue at C-7 and N-methyltetrazolyl-thiomethyl residue at C-3 showed a high order of antibacterial activity in vitro and especially in vivo.

Animals↗
PubMed1983

Chemical synthesis of a human fibroblast interferon gene and its expression in Escherichia coli.

Using the solid phase phosphotriester method, a gene coding for human fibroblast interferon consisting of 166 amino acid residues was chemically synthesized. The gene obtained by ligation of 61 synthesized oligodeoxyribonucleotide fragments was inserted into the downstream of tryptophan promoter, and expressed in E. coli. Lysate of this E. coli showed the antiviral activity which was specifically neutralized by anti-fibroblast interferon antibody. No particular advantage was observed in the expression efficiency by the synthetic gene over that by the native gene.

Amino Acid Sequence↗
PubMed1982

Modification of the macrolide antibiotic midecamycin. III. Formation of neoisomidecamycin.

Treatment of 9-trichloroacetylmidecamycin with aqueous alkali gave an isomer of midecamycin (neoisomidecamycin), in which one double bond underwent allylic rearrangement. The formation of neoisomacrolides was dependent on the nature of protective groups and conditions of deprotective reaction. Treatment of 9-trichloroethoxycarbonylmidecamycin with Zn/acetic acid resulted in no allylic rearrangement.

Anti-Bacterial Agents↗
PubMed1981

Studies on SF=1902 A2 A5, minor components of SF-1902 (globomycin).

Four members of globomycin, SF-1902 A2, A3, A4a and A4b were newly isolated from the culture of Streptomyces hygroscopicus SF-1902. These minor components shared four amino acids in common and the fifth was either valine or allo-isoleucine. The fatty acid moiety varied from 3-hydroxy-2-methylheptanoic acid in A2 to 3-hydroxy-2-methylundecanoic acids in A4b. The length of alkyl chain greatly affected the antibacterial activity, and maximum activity was shown by the homologue (A5) possessing the longest alkyl chain.

Acetylation↗
PubMed1980

Changes in molecular species of rat liver choline glycerophospholipids with development.

Molecular species of rat liver choline glycerophospholipids were investigated at various stages of development by a gas chromatograph-mass spectrometer system. They were composed mainly by 1,2-diacyglycerophosphocholine and changed with development particularly during the perinatal period.. In the prenatal period, the major species were '32 : 0' (mainly 16 : 0/16 : 0, dipalmitoyl species), '34 : 0' (mainly 16 : 0/18 : 0, palmitoystearoyl speciesY, '34 : 1' (mainly 16 : 0/18 : 1, palmitoyloleoyl species) and '34 : 2' (mainly 16 : 0/18 : 2, palmitoyllinoleoyl speciesY and '32 : 0' decreased rapidly and '34 : 1' increased as the stage proceeded. After birth, however, polyunsaturated species such as '34 : 4' and '38 : 4-6' increased rapidly in contrast to the decrease of '32 : 1, 34 : 1, 36 : 1, and 34 : 0'. Moderate changes were observed in these species during subsequent development.

Animals↗
PubMed1980

SF-1961, a new antibiotic related to bleomycin.

A new type of bleomycin group antibiotic, SF-1961 was isolated from the fermentation broth of Streptomyces species. SF-1961 possessed beta-amino-beta-(4-amino-6-carboxypyrimidin-2-yl)-propionic acid instead of its 5-methyl derivative which was a common component of bleomycins and phleomycins.

Bacteria↗
PubMed1979

Studies on molecular species of choline glycerophospholipids of developing rat brain.

The chronological changes in molecular species of choline glycerophospholipids were studied for cerebra of 17-, 19- and 21-day-old rat fetuses, and 3-, 6-, 12-, 24- and 90-day-old rats. The molecular species found by gas chromatography-mass spectrometry and selected ion retrieval technique were phosphatidylcholines of '30 : 0, 32 : 0, 32 : 1, 34 : 0, 34 : 1, 34 : 2, 36 : 0, 36 : 1, 36 : 2, 36 : 3, and 36 : 4' where the larger number indicates the sum of chain lengths on positions C-1 and C-2; the smaller number is the total number of double bonds. Of these molecular species, '32 : 0' (mainly 16 : 0/16 : 0, dipalmitoyl glycerophosphorylcholine), '34 : 1' (mainly 16 : 0/18 : 1, palmitoyloleoyl glycerophosphorylcholine), '34 : 0' (16 : 0/18 : 0, palmitoylstearoyl glycerophosphorylcholine), '32 : 1' (mainly 16 : 0/16 : 1, palmitoylpalmitoleoyl glycerophosphorylcholine and '30 : 0' (14 : 0/16 : 0, myristoylpalmitoyl glycerophosphorylcholine) were main species. The '32 : 0' species increased to about 44% at around the 10th day and thereafter remained nearly constant. '34 : 1' and '34 : 0' decreased to about 17 and 6% at that time and then increased to about 30 and 14%, respectively. '30 : 0' increased from last stage of gestation to the 6th day and then decreased. '32 : 1' was about 16% for 17-day-old fetus and decreased grandually. '36 : 1' (18 : 0/18 : 1, stearoyloleoyl glycerophosphorylcholine) increased at the latter part of development.

Aging↗
PubMed1977

Studies of molecular species of fetal lung lecithins by gas-liquid chromatography mass spectrometry.

The molecular species of lung lecithins obtained from rabbit and rat fetuses at different days of gestation and from newborns were analysed as TMS derivatives of the corresponding diglycerides in a gas chromatograph mass spectrometer system by selected ion retrieval. In the latter part of gestation, the order of the main molecular species of lung lecithins, PCn, was PC32 greater than PC34 greater than PC30, while at an earlier stage it was PC34 greater than PC32 greater than PC30. During gestation the change of PC32:0, dipalmitoyllecithin, was remarkable. Ether lecithins are also discussed.

Animals↗