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Biomedical subjects

H Ogino

Publications and source records attributed to H Ogino.

At least 181 records · Page 10Linked to original sources
PubMed1990

[The influence of playing the clarinet on the dentomaxillofacial morphology and function].

The purpose of this experiment is to understand the influence of playing the clarinet on the dentomaxillofacial morphology and function. The 12 subjects, selected at random (all adults, 4 men and 8 women) had played the clarinet for more than 10 years. The subjects had anamnesis, oral photo pictures, facial photo pictures, cast model, lateral cephalograms (lateral cephalo) and frontal cephalograms (frontal cephalo) taken when they were in centric occlusion and playing the clarinet. The results were as follows: 1. The facial profile and occlusal relation depend on the subjects. (2 mandibular retrognathism. 4 prognathism and 6 crowding.) 2. No characteristic skeletal pattern was found compared with controls in angle measurement but subjects had a tendency to have large facial height in linear measurement of lateral cephalo. And in denture pattern the lower incisors were linguoclination. 3. Concerning the lateral cephalo, we noticed that the angle of the clarinet in relation to the body axis increased in accordance with the prognathism and decreased with the retrognathism. 4. The subjects don't have identical midline, comparing lower midline to dentofacial midline, when playing, the angle of clarinet to the body was eccentric according to maxillary incisors in frontal cephalo. 5. Anterial and posterial length was short in mandibular arch, analyzing the cast model. 6. The pressure on mouthpiece increased in accordance with the prognathism and decreased with retrognathism. 7. The results of the EMG analysis indicated that the muscle activity of oral sphincter was described as 1). upper lip, 2). lower lip, 3). commisure of lips and differences were found depending on the parts being studied and the sound played on the clarinet. In conclusion, the skeletal and denture problem influence the holding position of the clarinet, embouchure and the way of playing it. In case of mandibular prognathism, when playing, the subjects pressed on their teeth with the clarinet. So B instrument won't do for prognathism because lower incisors are pushed inward as a result.

Adult↗
PubMed1990

[Changes in the immune response in postoperative patients while running the ventricular assist device (VAD)].

We examined the immunological parameters in two patients who were assisted with LVAD in postoperative LOS. Both patients demonstrated transient consumption of complements immediately after running the LVAD. The serum concentrations of immunoglobulins (IgG, IgM, IgA) showed no significant decrease. Both patients had significant postoperative lymphopenia, but recovered in a week. A decrease in the number of T cells was observed in both patients. The OKT4/OKT8 ratio initially increased in one patient, but gradually declined postoperatively. In the other patient, the OKT4/OKT8 ratio decreased. The lymphocyte response to mitogen (PHA) remained significantly depressed when the number of lymphocytes returned to the normal level, suggesting that the lymphocytes were functionally impaired. Such findings are generally observed after cardiac surgery. Thus, LVAD does not affect the immune response.

Adolescent↗
PubMed1989

Characteristic incorporation of ganglioside GM3, which induces monocytic differentiation in human myelogenous leukemia HL-60 cells.

Using tritiated gangliosides [( 3H]-GM3 and [3H]-GM1), characteristic incorporation of exogenous GM3 to HL-60 cells was demonstrated in association with differentiation induction. [3H]-GM3 was bound 4-5 times more than [3H]-GM1 was. Scatchard analysis revealed high and low affinity patterns of binding to the cells. The concentration of GM3 that caused growth inhibition and cell differentiation corresponded well to that which showed the bi-phasic binding pattern. It was strongly suggested that GM3, which induces monocytic differentiation, was characteristically bound and incorporated to the cells around the concentration which caused growth inhibition and cell differentiation.

Cell Differentiation↗
PubMed1989

Experimental induction of uterine cancer in rats by N-ethyl-N'-nitro-N-nitrosoguanidine dissolved in polyethylene glycol.

In the present experiment we attempted to experimentally induce uterine cancer in rats by injecting into the uterine cavity N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) dissolved in polyethylene glycol (PEG). Fifty-nine female F-344 rats, 7-8 weeks old, were divided into three groups and each received in the left uterine cavity with laparotomy a single dose of ENNG dissolved in PEG according to the following schedule: Group 1 received 75 mg ENNG/kg body wt.; Group 2 had 20 mg ENNG/kg body wt.: and Group 3 was given only PEG. In Group 1 it was observed that adenocarcinoma and sarcoma were present in the uterine corpus while squamous cell carcinoma occurred in the uterine cervix. In Group 2, although tumors such as adenocarcinoma, adenoma and sarcoma were observed in the uterine corpus, no tumor was present in the uterine cervix. No tumor growth whatsoever was observed in Group 3. From the above results it is apparent that the present method is an efficient means for experimentally inducing uterine cancer and that the site of tumor generation varies according to the concentration of ENNG administered.

Animals↗
PubMed1989

[Aortic aneurysm associated with coarctation of the aorta].

An 8-year-old female of coarctation of the aorta associated with thoracic aortic aneurysm is reported. Under cardiopulmonary bypass, a 7 cm section of the aorta containing the aneurysm and coarctation was resected and replaced by a 16 mm woven Dacron tube. The postoperative recovery was complicated by chylothorax treated by thoracic aspiration. She is doing well 3 months after the operation. Histologic sections of the resected portions of the thoracic aorta revealed intimal hyperplasia, medial fibrosis and decrease of elastic fibers, especially in the aneurysm.

Aorta, Thoracic↗
PubMed1989

[Long-term observation of two post-treatment cases with an impacted maxillary central incisor corrected by orthodontic treatment].

UNLABELLED: This article describes long-term observation of two post treatment cases with an impacted maxillary central incisor corrected by orthodontic treatment. [Case 1] Age at first examination: 11 years and 1 month. Chief complaint: Retarded eruption of a maxillary right incisor and crowding of teeth. DIAGNOSIS: No skeletal problems were identified. Crown axis inclination of the impacted tooth showed 104.0 degrees. Root axis inclination showed 87.0 degrees. Treatment plan: [formula; see text] extraction, traction of the maxillary right incisor, edgewise method. Treatment time: 4 years and 3 months. [Case 2] Age at first examination: 8 years and 8 months. Chief complaint: Retarded eruption of a maxillary left incisor. DIAGNOSIS: No skeletal problems were identified. Crown axis inclination of the impacted tooth showed 112.0 degrees. Root axis inclination showed 88.0 degrees. Treatment plan: Traction of maxillary left incisor, edgewise method. Treatment time: 5 years and 6 months. The findings were as follows; 1) When the active treatment was finished, no pulp necrosis nor any resorption of root or alveolar bone was found in the two cases. 2) At present, the crown axis inclination is restored to normal in both cases. 3) The impacted maxillary central incisors were treated to the normal position in the dental archs, and the condition and the tissues around are good. The foregoing results, we are sure, can be an important traction of the impacted maxillary central incisor.

Cephalometry↗
PubMed1989

[Late results after heart valve surgery in the elderly].

Heart valve surgery was performed in 133 patients over the age of 60 between 1976 and 1981. There were 54 men and 79 women. Their ages ranged from 60 to 74 years (mean age 64.3 years). In this study, 54 valve prostheses (15 porcine and 39 mechanical) in the aortic position, 79 prostheses (69 porcine and 10 mechanical) in the mitral position and 3 prostheses (3 porcine) in the tricuspid position were implanted in 121 patients. Fifteen patients (11.3%) died in the hospital. The hospital mortality was high in the cases of MVR (14.6%), MVR + TAP (12.5%) and emergency (50%). The mean follow-up was 37.2 months (range 4 to 129 months, total 367.3 patient-years). There were 10 late deaths (8.5%). Actuarial survival for hospital survivors at 5 years was 89.2 per cent. At follow-up, 95.8% of the surviving patients were in New York Heart Association functional class I or II. Valve-related complications were thromboembolism (2.0% pt/year), periprosthetic leak (1.7% pt/year), primary tissue failure (0.5% pt/year) and thrombosed valve (0.3% pt/year). Anticoagulant-related hemorrhage necessitating hospitalization occurred in 2 patients (1.0% pt/year). The freedom from all events at 5 years was 72.8 per cent. This study suggests that heart valve surgery in the elderly can be performed with an acceptable mortality. Advanced age alone should not be a contraindication to surgical management.

Aged↗
PubMed1988

Expression and excretion of human pancreatic secretory trypsin inhibitor in lipoprotein-deletion mutant of Escherichia coli.

We constructed a gene coding for the 56-amino acid human pancreatic secretory trypsin inhibitor (PSTI), and ligated it on a plasmid downstream from the trp promoter and the signal peptide sequence of alkaline phosphatase. The resulting plasmid was transfected into a lipoprotein deletion mutant (Escherichia coli JE5505) and the plasmid-carrying cells were induced with 3-indoleacrylic acid. A considerable amount (50 micrograms/ml culture) of the mature PSTI protein was detected in the culture supernatant. The excreted PSTI was identical to the natural PSTI protein with respect to the trypsin-inhibiting activity, the N-terminal and the C-terminal amino acid sequences and the amino acid composition.

Base Sequence↗
PubMed1988

Synergistic effect of lymphokines and lipopolysaccharides on macrophage chemiluminescence. Appearance of spontaneous chemiluminescence and its correlation with cytotoxicity.

A new type of chemiluminescence (CL) was observed in thioglycolate-elicited ICR mouse peritoneal macrophages which had been incubated for 24 h in medium containing lymphokines (LK) and lipopolysaccharides (LPS), and then exposed to a CL reagent solution containing luminol and phorbol myristate acetate (PMA). The new CL, which was clearly distinguishable from PMA-induced CL, appeared immediately after the addition of the CL reagent solution, reaching a maximal level at about 30 s, and disappeared rapidly. We have called the new CL 'spontaneous CL', since it appeared spontaneously without PMA triggering. When the macrophages were incubated with LK in the presence of 10 ng/ml of LPS, the spontaneous CL began to appear after about 4 h incubation, the maximal level being reached at about 12 h, after which it decreased gradually on further incubation. After 48 h, it could not be observed at all. The spontaneous CL could be observed only in macrophages simultaneously treated with LK and LPS, as in the case of cytotoxicity. The correlation between spontaneous CL and cytotoxicity was suggested by the following; they showed a similar dose dependency to LK, and neither of them could be induced in aged macrophages which had been incubated in vitro for 1 day before exposure to LK and LPS. These results suggest that spontaneous CL measurement could possibly replace the cytotoxicity test as a simple method for the determination of macrophage activating factor.

Animals↗
PubMed1988

Application to forensic odontology of aspartic acid racemization in unerupted and supernumerary teeth.

Racemization of aspartic acid in dentin protein during the human lifetime progresses with age. The extent of racemization of aspartic acid in coronal dentin of normal permanent teeth can be used in forensic odontology to estimate the age of an individual at the time of death (Ogino et al., 1985). A series of experiments was conducted with dentin separated from unerupted and supernumerary teeth of various ages in an attempt to evaluate the advantages and limitations of this age-estimation method. The current study on nine tooth specimens showed that some unerupted permanent teeth with normal-sized and -shaped crowns (impacted third molar, canine, and incisor) could be used to estimate the age of individuals at the time of death within +/- 4 years. However, supernumerary teeth (mesiodens, paramolar) with extremely tiny (length of crown: 4 approximately 5 mn) and abnormally shaped crowns could not be used for analysis. In such cases, the estimated age of individuals analyzed by the racemization method deviated considerably from their actual age.

Adolescent↗
PubMed1987

Influence of ptaquiloside on the development of newborn mice.

We attempted to induce neurological disorders in mice by a single administration of various doses of ptaquiloside (PT) within 24 h after birth. Animals which survived for a long period after treatment showed a reduction in body weight, but did not develop any gait disturbance. On the other hand, in treated animals which died before beginning to walk, karyorrhexis in the external granular layer of the cerebellum and karyorrhexis and rosette formation in the neuroblastic layer of the retina were present. In addition, treated animals showed karyorrhexis and great reduction in the number of the cells in the thymus, suggesting cytotoxic and cytolytic effects of alkylating agents on lymphocytes in this organ.

Animals↗
PubMed1987

Thoracic myelopathy secondary to ossification of the spinal ligament.

The authors describe their experience with 26 cases of thoracic myelopathy secondary to hypertrophic ossification of the spinal ligament (posterior longitudinal ligament and/or ligamentum flavum). The clinical manifestations of this condition and results of its surgical treatment are described. The commonest symptoms were numbness or tingling in the legs and feet and gait disturbance. Most of the patients with involvement of the upper thoracic spine showed typical features of thoracic myelopathy: that is, sensory and motor deficits in both the trunk and lower extremities, sphincter disturbance, and exaggerated tendon reflexes. Several patients with involvement of the thoracolumbar junction presented with atypical symptoms of thoracic myelopathy and were sometimes misdiagnosed and treated inappropriately. Surgical treatment, particularly laminectomy, was not always successful. Inconsistencies in the surgical outcome were caused by either operative complications or reversal of the initial improvement during the follow-up period. The results of anterior surgery for the condition were more favorable; however, use of this procedure was rarely indicated.

Adult↗
PubMed1987

The inhibition of neoplastic cell proliferation with human natural tumor necrosis factor.

Purified human natural tumor necrosis factor (n-TNF) was prepared by stimulating human leukemic B cell line (BALL-1) with Sendai virus. The colony formations of all of 18 human cancer-derived abnormal cell lines were suppressed by 10(1)-10(6) U/ml of n-TNF, while n-TNF was nontoxic to all human normal fibroblast cells. This in vitro inhibition of cell growth was reversible. In breast adenocarcinoma MCF7 cells treated with n-TNF a specific decrease of DNA synthesis was observed, and DNA histograms showed a block at G1 in the cell cycle. In vivo studies revealed that n-TNF suppressed the tumor growth of murine Meth A sarcoma, human renal adenocarcinoma (ACHN), malignant melanoma (SK-MEL-28) and glioblastoma (U-373 MG). Isobologram analysis showed that n-TNF synergistically inhibited cell growth in combination with human natural interferon (IFN)-a. In vivo synergism of n-TNF and IFN-a was also found in the U-373 MG tumor model implanted into nude mice.

Animals↗
PubMed1987

[A parallel comparative double blind study of cefixime with cefroxadine in the treatment of acute lacunar tonsillitis].

The clinical efficacy and safety of cefixime (CFIX), a new oral cephalosporin, were compared with those of cefroxadine (CXD) in patients suffering from acute lacunar tonsillitis in a double blind study. Two hundred and fifty two patients were given each orally 100 mg of CFIX b.i.d. or 250 mg of CXD t.i.d. for, in principle, 7 days. Number of patients evaluated for clinical efficacy was 202 (103 treated with CFIX and 99 treated with CXD). As for the backgrounds of patients, more severe cases were found in the CFIX group than in the CXD group (P less than 0.01). Efficacy rates evaluated by individual doctors were 88.3% in the CFIX group and 91.9% in the CXD group. There was no significant difference between the 2 groups. Efficacy rates on the third day after the initiation of treatment evaluated by the committee were 40.8% in the CFIX group and 47.9% in the CXD group with no significant difference. Efficacy rate on the 7th day, however, was 79.8% in the CFIX group and 93.4% in the CXD group, showing a significant difference (P less than 0.05). Bacteriological effectiveness were satisfactory for both groups with eradication rates of 93.4% for the CFIX and 96.9% for the CXD group. Number of patients evaluated for safety was 226 (110 treated with CFIX and 116 treated with CXD). No significant difference was observed between the 2 drug groups in incidences of side effects; gastrointestinal disturbances or rashes were noted in 6 patients (5.5%) of the CFIX group and in 5 patients (4.3%) of the CXD group. As for the abnormal laboratory findings, elevation of GOT & GPT was observed in 1 patients of the CFIX group. From these results, it was concluded that 100 mg b.i.d. of CFIX was as useful as 250 mg t.i.d. of CXD in the treatment of acute lacunar tonsillitis.

Acute Disease↗