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Biomedical subjects

H Ochiai

Publications and source records attributed to H Ochiai.

At least 181 records · Page 10Linked to original sources
PubMed1991

[The treatment of condyle neck fracture: statistics gathered by multi centric study and the related prognosis].

There are two different treatments for condyle neck fracture. One is non-surgical treatment involving intermaxillary fixation, and the other is radical surgical treatment through various techniques, but it is difficult to discuss the relative merits. The purpose of this report is to decide whether surgical treatment is necessary, by comparing the two types of treatment. In the period between April 1981 to December 1987, we treated 136 cases of condyle neck fracture at the Second Department of Oral and Maxillofacial Surgery School of Dentistry Aichigakuin University, and in 18 nearby hospitals. Of the 136 cases, 58 cases had fracture only at the condyle neck, while 78 cases had some concomittant fracture. As regards treatment, of the 136 cases, 49 (36%) were treated surgically, while 87 (64%) were treated non-surgically for condyle neck fracture. The surgically treated cases were divided into three groups: 24 cases were treated by pinning, 16 cases were treated by inter osseous wiring, and there were 9 other cases. In the treatment of concomittant fracture, 38 cases involved inter osseous wiring, 22 cases involved metal plate fixations, and there were 4 other cases. Non-surgical treatment accounted for 15 cases. The rate of surgical treatment for concomittant fracture was 82.1% of the cases treated. In surgically treated cases of condyle neck fracture, the mouth opening prognosis was generally poor. However, in these cases the deviation of mouth opening was generally better than in non-surgically treated cases. There were no differences in mastication, occlusion and pain in the TMJ region.

Bone Wires↗
PubMed1991

[Mechanism of the B-B' step formation in M-mode echocardiography].

This study analyzed the mechanism involved in the B-B' step formation of the mitral valve movement using pulsed Doppler and color Doppler echocardiography. We examined 24 patients with first degree atrioventricular block (AVB), 26 with old myocardial infarction (OMI), and 34 with a DDD-pacemaker (PM) (16 with complete AV block and 18 with sick sinus syndrome). In 19 of 24 AVB patients, the B-B' step was associated with diastolic mitral regurgitation (DMR). There was a significant positive correlation between the magnitude of the PQ interval and the B-B' step duration (r = 0.94, p less than 0.001). Intravenous administration of 1 mg of atropine shortened the B-B' step duration from 0.15 +/- 0.06 (SD) to 0.05 +/- 0.07 sec (p less than 0.001). Although changes in the PQ interval were well correlated with changes in the B-B' step duration, the P-B-B' step, or PC interval, did not change significantly. In 12 of the 26 OMI patients, the B-B' step and DMR were both observed. The QC intervals of these patients were longer than those of OMI patients without the B-B' step (0.088 +/- 0.03 vs. 0.066 +/- 0.021 sec, p less than 0.05). The P-B-B' step interval was shorter in the OMI patients than in the AVB patients (0.190 +/- 0.029 vs 0.232 +/- 0.033 sec, p less than 0.01). In 28 of 34 patients implanted with a DDD-pacemaker, the B-B' step and DMR were induced by prolonging the AV delay.(ABSTRACT TRUNCATED AT 250 WORDS)

Coronary Circulation↗
PubMed1990

Ultrastructural demonstration of dopamine-beta-hydroxylase immunoreactive nerve terminals on vasopressin neurons in the paraventricular nucleus of the rat by double-labeling immunocytochemistry.

The noradrenergic innervation of vasopressin (VP) neurons in the paraventricular nucleus (PVN) of the rat was studied ultrastructurally by double-labeling immunocytochemistry combining the preembedding peroxidase-antiperoxidase method for dopamine-beta-hydroxylase (DBH) with the post-embedding immunogold staining method for neurophysin II, the carrier protein of VP. DBH-like immunoreactive nerve terminals were found to make synaptic contacts with neurophysin II-like immunoreactive neuronal perikarya and their processes. This provides morphological evidence for noradrenergic control of the release of VP, at the PVN of the rat.

Animals↗
PubMed1990

Shedding of CD9 antigen into cerebrospinal fluid by acute lymphoblastic leukemia cells.

The accurate identification of small numbers of leukemic cells in the cerebrospinal fluid (CSF) presents a diagnostic problem in the treatment of acute lymphoblastic leukemia (ALL). We demonstrated that soluble CD9 antigen was shed into CSF obtained from children with ALL, using enzyme-linked immunosorbent assay (ELISA), which used the activity of CD9 antigen to bind the Ricinus communis agglutinin (RCA1) and a monoclonal antibody, SJ-9A4, simultaneously. Using RCA1/SJ-9A4 ELISA, CD9 antigen was detectable in CSF but not in plasma from 12 cases of CD9+ ALL in central nervous system (CNS) relapse. However, CD9 antigen was not released into CSF from 11 cases of CD9- ALL with CNS involvement, 136 cases of CD9+ ALL in complete remission (CR), 29 cases of CD9- ALL in CR, or 21 cases of aseptic meningitis. Interestingly, the levels of CD9 antigen were elevated in CSF from 7 of 10 CD9+ ALL patients without cytologically proven CNS involvement at diagnosis, with subsequent return to undetectable levels after initial induction chemotherapy was begun. In addition, sequential analysis of CSF from a 5-year-old boy with CD9+ ALL in CNS relapse showed that levels of CD9 antigen correlated well with the number of leukemic cells in CSF. Serial quantitative analysis of CD9 antigen in CSF could be useful to detect the proliferation of residual leukemic cells before the clinical manifestation.

Antibody Specificity↗
PubMed1990

Recovery of the contact site A glycoprotein of Dictyostelium discoideum from sodium dodecyl sulfate-polyacrylamide gel and characteristics of monoclonal antibodies against the recovered protein.

Monoclonal antibodies were produced against a cell-cell adhesion (contact site A) glycoprotein of Dictyostelium discoideum, isolated by preparative gel electrophoresis. The glycoprotein was recovered by electroelution from a polyacrylamide gel strip and used for the production of monoclonal antibodies. Four of the five antibodies obtained bound specifically to the protein moiety of the contact site A glycoprotein. The specificities of the antibodies were in striking contrast to those of antibodies raised against the contact site A glycoprotein purified by Triton X-114 phase separation and DEAE chromatography. The majority of the latter antibodies recognized the carbohydrate moiety of the contact site A glycoprotein and cross-reacted heavily with other membrane glycoproteins.

Antibodies, Monoclonal↗
PubMed1990

Infection enhancement of influenza A H1 subtype viruses in macrophage-like P388D1 cells by cross-reactive antibodies.

The contribution of cross-reactive hemagglutination inhibition (HI) antibodies to infection enhancement of influenza A H1 subtype NWS virus and two antigenic drift strains was investigated in a macrophage-like cell line P388D1. When P388D1 cells, previously treated with neuraminidase (NA) to remove the viral receptors, were infected with NWS virus exposed to rabbit antiviral immunoglobulin (IgG) showing various levels of cross-HI titers, virus yields were enhanced in the presence of a subneutralizing antibody, depending on their cross-HI titers. By flow cytometric analysis using a fluorescein isothiocyanate (FITC)-labeled NWS virus, the efficiency of attachment of virus-rabbit IgG complexes to Fc receptors on NA-treated cells showed close correlation with its cross-HI titer. These data suggest that cross-reactive HI antibodies could contribute to infection enhancement through the formation of potent infectious immune complexes with drift strains to mediate virus infection via Fc receptor uptake. Two monoclonal antibodies (mAB) in mouse IgG subclasses IgG1 and IgG2a showing strain-specific or cross-reactive HI activity were tested for their infection enhancement characteristics. A strain-specific mAB enhanced infection of homologous NWS virus, but not that of two other drift strains in either antibody dilution. In contrast, a cross-reactive mAB caused infection enhancement of all three virus strains in the presence of the subneutralizing antibody. This indicates that cross-reactivity, but not the IgG subclass, acts as an enhancing factor to this phenomenon. The antibody, with the same specificity as cross-reactive mAB, was detected semiquantitatively by competitive enzyme-linked immunosorbent assay (ELISA) with results almost consistent with cross-HI titers of polyclonal rabbit antiviral IgGs. These data suggest that the antibody detected by this assay might be one of the potent antibodies governing cross-HI activity as a whole antibody and causing infection enhancement of drift strains.

Animals↗
PubMed1990

Monoclonal antibodies against the mercaptoethanol-sensitive structure of a cell-cell adhesion protein of Polysphondylium pallidum.

Monoclonal antibodies were prepared against a putative cell-cell adhesion glycoprotein, with an apparent molecular mass of 64,000 (gp64), of the cellular slime mold, Polysphondylium pallidum. Five monoclonal antibodies obtained by means of an enzyme-linked immunoadsorbent assay did not bind to the antigens which were subjected to gel electrophoresis and blotting method in the presence of a reducing agent, but they did bind specifically to the antigens prepared in unreducing conditions of samples and then processed by the same blotting method. To solubilize gp64 in a sodium dodecyl sulfate (SDS)-sample buffer without mercaptoethanol (heated) or SDS-sample buffer with 2-mercaptoethanol (nonheated) was critical for the antibody binding onto gp64 on a membrane. Hence the antibodies seem to bind to a surface portion(s) of the localized protein structure folded up by disulfide cross-linkages. One of the antibodies obtained blocked cell-cell adhesion by about 20%.

Animals↗
PubMed1990

Inhibitory effect of protein kinase C inhibitor on the replication of influenza type A virus.

The growth of influenza virus A/PR/8/34 in MDCK cells was inhibited by 1-(5-isoquinolinesulphonyl)-2-methylpiperazine dihydrochloride (H7) which is a potent inhibitor of protein kinase C, but not by an effective inhibitor of cyclic nucleotide-dependent protein kinases. Analysing the inhibitory effect of H7 during the replication cycle of influenza virus, we found that the primary transcripts were sufficiently synthesized in infected cells exposed to H7. The primary transcripts synthesized in the presence and absence of H7 were active in directing the synthesis of viral polypeptides both in a cell-free system and in the system containing H7. In the system where infected cells were exposed to H7, the viral positive-sense RNAs were also significantly amplified 6 h after infection. However, the synthesis of viral proteins other than nucleoprotein from viral primary or amplified (secondary) mRNAs was extremely restricted. The synthesis of host cellular proteins in mock-infected cells was significantly retained in the presence of H7. These results suggest that the selective inhibition of influenza virus translation following the transcription of viral mRNA was induced by H7 in infected cells.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗
PubMed1990

Purification and characterization of a novel brain-specific 14-kDa protein.

A new acidic protein specifically present in the brain was purified to homogeneity from bovine brain. The apparent molecular mass was estimated to be 14 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 57 kDa by gel filtration, a finding suggesting that it exists as a tetramer under physiological conditions. The protein had a high content of Glu and Pro, and its pI was 4.3. The first six amino acid residues of the protein were Met-Asp-Val-Phe-Met-Lys, and the amino terminal was blocked. The distribution of the protein examined by Ouchterlony gel immunodiffusion indicates that it is present specifically in brain, including rat, human, and bovine, but could not be detected in 10 other rat tissues examined. The protein was absent in Purkinje cell bodies, as examined by electron microscopic immunocytochemistry, but was present in nerve terminals that make synapse-like contacts with Purkinje cells and in neurons with dark granules in the globus pallidus of the rat.

Amino Acid Sequence↗
PubMed1990

Cytotoxic and antiherpetic activity of phloroglucinol derivatives from Mallotus japonicus (Euphorbiaceae).

The phloroglucinol derivatives isolated from Mallotus japonicus Muell. Arg. (Euphorbiaceae) and their derivatives were evaluated for their capacity to produce cytotoxicity in HeLa cells and to inhibit the replication of herpes simplex virus type 1 (HSV-1). The characterizations of an isolated new acetophenone, mallophenone (16), and cyclization products of mallotojaponin (1), isomallotochromene (17), mallotochroman (18) and isomallotochroman (19), were also described. All tested derivatives inhibited the replication of HSV-1 with ED50 in the range of 88 ng--48 micrograms/ml. The derivatives 12 and 19 were found in vitro therapeutic index with 10.9 and 9.1, respectively, and they were considered to be active antivirals.

Antiviral Agents↗
PubMed1990

[Solubilizing effect and inclusion reaction of cyclic bisdesmosides from tubers of Bolbostemma paniculatum].

Tubeimosides I, II and III (cyclic bisdesmosides) have been isolated from Chinese cucurbitaceous crude drug Tu-bei-mu, a tuber of Bolbostemma paniculatum (Maxim.) Franquet. Solubilizing effects of these cyclic bisdesmosides on water insoluble or less-soluble compounds were examined. It was revealed that cyclic bisdesmosides were effective on increasing the solubility of Yellow OB, dl-alpha-tocopherol and saponin A from Sapindus mukurossi. The critical micell concentration (cmc) and association number as well as diameter of micell of tubeimoside I in water were also measured. The interaction of tubeimoside I with 1-anilino-8-naphthalene-sulfonate (ANS) in aqueous solution was investigated photometrically. It was observed that tubeimoside I strongly enhanced the intensity of fluorescence of ANS, suggesting the significant formation of inclusion complex.

Drugs, Chinese Herbal↗
PubMed1990

[Torsade de Pointes with T wave alternans in a patient receiving moderate dose of chlorpromazine: report of a case].

A case is presented of Torsade de Pointes (TDP) with T wave alternans in a 31-year-old female receiving a moderate dose of chlorpromazine. She was treated in an another hospital for schizophrenia with chlorpromazine (100 mg daily) for several years and admitted to Fujisawa city hospital for numerous episodes of syncope. The electrocardiogram immediately after admission revealed a marked QTc prolongation to 0.81 seconds, T wave alternation without any obvious change in morphology of the QRS complex, and recurrent ventricular tachycardia called TDP. The T wave alternans and TDP were easily abolished by intravenous administration of a bolus of 50 mg lidocaine infusion. The QT interval however, remained prolonged. Physical examination, including cardiac examination, was normal. Serum potassium was 3.6/mEq. Chlorpromazine was discontinued immediately after admission and no further episodes of TDP were seen after the first day. After the QT interval returned to almost normal, chlorpromazine (50 mg daily) was re-administered. Two days after the re-administration, the electrocardiogram revealed marked QT interval prolongation with prominent T waves. Psychotropic drugs, such as chlorpromazine, prolong the QT interval and cause TDP. Chlorpromazine appears to have been responsible for TDP and the T wave alternans in this case. TDP caused by a moderate dose of chlorpromazine has not been previously reported. Lone T wave alternans unaccompanied by changes in the QRS complex is a rare phenomenon and the mechanism underlying T wave alternans remains unknown.

Adult↗
PubMed1990

[Atrioventricular delay and diastolic mitral regurgitation in patient with DDD pacemaker implantation, and cardiac function].

We examined the mitral valve motion, the mitral valve flow pattern, and changing atrioventricular (AV) delay in 34 patients with DDD pacemaker implantation, using M-mode echocardiography and pulse Doppler echocardiography. In 28 patients, B-B' step and diastolic mitral regurgitation (DMR) were induced by extending the AV delay. 8 of 12 patients who showed B-B' step and DMR with the AV delay less than or equal to 165 mS had heart failures. In such patients, P-B-B' step (DMR) interval was 174 +/- 31 mS shorter than the other patients (216 +/- 26 mS, p less than 0.001), and Q-C interval was 131 +/- 26 mS longer than the other patients (63 +/- 31 mS, p less than 0.001). The minimal value of the AV delay that induces B-B' step and DMR can be decided under the influence of cardiac functions, and this echocardiographic examination is useful, giving us information about the cardiac function of the patients with DDD-PM. B-B' step is the mitral valve motion that corresponds with the DMR, so we can check the DMR through the B-B' step easily.

Aged↗
PubMed1990

[A case of myotonic dystrophy associated with intracardiac conduction abnormalities and aortic regurgitation].

A rare case of myotonic dystrophy with mechanical derangement in a 48-year-old male is reported. The patient was admitted to Fuzisawa City Hospital because of chest discomfort. On physical examination, he had the typical facial appearance of myotonic dystrophy, and displayed grip myotonia. The blood pressure was 120/60 mmHg and the pulse 51, regular. A systolic ejection murmur at the cardiac base, an early diastolic blowing murmur along the left sternal border, and a pansystolic murmur at the apex were heard. The deep tendon reflexes were all normal. Elevation of serum creatine kinase and aldolase were noted. Chest X-ray films suggested moderate cardiomegaly. An electrocardiogram showed sinus bradycardia, atrioventricular block and left bundle branch block, suggesting diffuse involvement of the conduction system. An echocardiography confirmed the presence of left ventricular enlargement, thickened aortic valves, mitral regurgitation, and aortic regurgitation. Selective coronary angiography revealed no abnormalities. Left ventriculography demonstrated diffuse hypokinesis of the entire ventricle. Light microscopy of biopsied right myocardium revealed prominent interstitial fatty infiltration, mild interstitial fibrosis, and variation in the nuclear size. A 22-year-old son also had myotonic dystrophy and had an echocardiography indicative of thickened valves with aortic regurgitation. Myotonic dystrophy is a autosomal dominant disease. Cardiac involvement selectively disturbs the conduction system, sinus node, and to a lesser extent myocardium. Although the presence of aortic regurgitation could be a mere coincidence, we believe that this did not occur by accident, because the patient's son also had aortic regurgitation.

Echocardiography↗
PubMed1989

Macrophage recognition of periodate-treated erythrocytes: involvement of disulfide formation of the erythrocyte membrane proteins.

Upon exposure to 2 mM periodate at 0 degrees C for 15 min, mouse erythrocytes underwent membrane lipid oxidation, oxidation of cell surface sialyl residues into aldehyde-bearing derivatives, and oxidation of SH groups of the membrane proteins into disulfides. The periodate-treated erythrocytes exhibited a remarkable increase in rosette attachment to resident mouse peritoneal macrophages in the absence of serum. The relationship between the oxidation of the membrane constituents and the macrophage recognition of these cells was investigated. Periodate treatment of erythrocytes in the presence of butylated hydroxytoluene, an inhibitor of lipid oxidation, did not affect the subsequent attachment of the erythrocytes to the macrophages. Reduction of the periodate-treated erythrocytes with borohydride or cyanoborohydride did not affect the erythrocyte attachment. Neuraminidase treatment of erythrocytes before periodate did not affect the attachment either. On reduction of the disulfides of the membrane proteins with dithiothreitol, the periodate-treated erythrocytes lost their ability to attach to the macrophages. Erythrocytes treated with an SH-oxidizing agent, diamide, were then examined for the macrophage recognition. The diamide-treated cells also showed rosette attachment to the macrophages in the absence of serum, but did not when reduced with dithiothreitol. These results indicate that oxidation of the SH groups of the membrane proteins to disulfides causes reversible membrane changes that macrophages recognize, and it is this mechanism that is responsible for the macrophage recognition of the periodate-treated erythrocytes.

Animals↗