Search PubMed⌕ Search

Biomedical subjects

H Nishimoto

Publications and source records attributed to H Nishimoto.

At least 73 records · Page 4Linked to original sources
PubMed1986

B cell-derived BCGF functions as autocrine growth factor(s) in normal and transformed B lymphocytes.

This paper demonstrates that B cell lines, as well as normal activated B cells generate and respond to B cell-specific growth factor(s) (BCGF). BCGF derived from B cells (B-BCGF) appears to be distinct from interleukin 1, interleukin 2 (IL 2), B cell stimulatory factor, BCGF-II, interferon-gamma, or transforming growth factor. It acts on activated B cells, but not on resting G0 phase B cells to induce proliferation. B cell lines, immortalized by Epstein-Barr virus, constitutively secrete 10-fold higher level of B-BCGF compared with normal activated B cells, suggesting that an activated autocrine loop might be operating in immortalized B cells. On the basis of our observations, we postulate that B cell clonal expansion may occur, at least in part, through a B-BCGF-dependent autocrine pathway similar to IL 2 effect on T cells.

Adjuvants, Immunologic↗
PubMed1986

Suppressive and augmentative effects of recombinant human interleukin-2 upon delayed type of hypersensitivity in the mouse.

The in vivo action of recombinant human interleukin-2 (IL-2) was investigated against the delayed type of footpad swelling to sheep red blood cells (SRBC) in B6D2F1 mice. A single intravenous injection of 1 X 10(5) of SRBC induced transient cell-mediated immunity for delayed type of hypersensitivity (DTH) which peaked four days after the immunization and diminished rapidly thereafter. IL-2 exerted both suppressive and augmentative effects, depending on the timing of its administration in relation to the immunization. Daily intraperitoneal injections of as much as 5 X 10(4) Jurkat units of IL-2 from day 0 through day 3 caused marked suppression of DTH elicited on day 4. On the other hand, three or four consecutive injections of IL-2 from day 4 or day 3 through day 6 produced potent DTH on day 7, when DTH was faint, if there was any, without IL-2 administration. Potentiation of DTH was also observed when three injections of IL-2 were given from day 7 through day 9 and the antigen was challenged on day 10. These results suggest that IL-2 acts as an immunomodulator against cell-mediated immunity to SRBC in the mouse.

Animals↗
PubMed1986

Effects of thalamic sensory relay nucleus stimulation on the jaw-opening reflex in response to tooth-pulp stimulation in the cat.

The effects of stimulation of the thalamic sensory relay nucleus (TSRN, nucleus ventralis posteromedialis) on the jaw-opening reflex (JOR) in response to tooth pulp stimulation were compared with the effects of stimulation of the periaqueductal gray (PAG) and nucleus raphe magnus (NRM) in the cat. After stimulation of the TSRN, PAG and NRM, the JOR was inhibited. However, while the inhibitory effects of PAG and NRM stimulation lasted for more than 500 ms and were antagonized by the opiate antagonist, naloxone, the inhibitory effects of TSRN stimulation lasted for approximately 100 ms and were resistant to naloxone. These findings suggest that although TSRN stimulation exerts descending inhibitory effects on segmental nociceptive activity, similarly to PAG or NRM stimulation, the descending inhibitory pathways mediating the effect of TSRN stimulation may be largely distinct physiologically as well as pharmacologically from those mediating the effect of PAG and NRM stimulation.

Animals↗
PubMed1984

[Thalamic relay nucleus stimulation for relief of intractable pain. Clinical results and beta-endorphin immunoreactivity in cerebrospinal fluid].

Chronic implantation of a stimulating electrode in the thalamic relay nucleus (11 cases), in the periaqueductal gray (1 case) and in the internal capsule (2 cases) was performed in fourteen cases which suffered from intractable pain. All these cases could get pain relief at least initial two months. Ventricular fluids were collected before and after stimulation with optimal combination of parameters, and measurements of beta-endorphin were performed by radio-immunoassay. Intrathecal morphine (1mg) injection was performed in eight cases. Cerebrospinal fluids were collected by lumbar tap before and 24 hours after morphine injection. beta-endorphin immunoreactivity was measured by the same method. Pain relief was judged to be excellent if the patient so claimed, and if he discontinued analgesics. Pain relief was thought to be good when it was not completely controllable by stimulation but was sufficiently improved that the patient could do without analgesics. It was thought to be fair when patient could not discontinue analgesics, and poor when patient could not get pain relief. We usually attempt to prevent the stimulation-tolerance by administration of the monoamine precursors , i.e., 1-dopa and 1-tryptophan, on the basis of the experimental observation reported previously. In somatogenic pain patients, the thalamic relay nucleus stimulation was performed in 7 cases (excellent; 3, good; 1, fair; 3) and the periaqueductal gray stimulation in one case (good).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1980

Assessment of brainstem damage by the auditory brainstem response in acute severe head injury.

In 64 cases suffering from severe head injury (Glasgow coma scale: less than seven- the auditory brainstem responses (FARs) recorded at the vertex, which are thought to be volumet conducted far-field potentials reflecting the sequential electrical activities of the auditory afferen) system in the brainstem, were recorded in the neurosurgical intensive care room immediately after admission. The alterations in the responses were compared with the types of primary injury, neurological signs., CT findings and outcome following treatment. Based on the results obtained, it is concluded that the FAR is a useful indicator for predicting the effects of treatment on brainstem damage in patients with severe head injury, and that it provides more reliable information about the function of the brainstem than the neurological signs or CT findings. Moreover, it also offers a diagnostic method for primary brainstem injury. Three cases or primary brainstem injury without lesions in the supratentorial region were diagnosed by means of combined CT and FAR recording.

Adult↗
PubMed1980

[Diagnostic value of the far field acoustic response (FAR) as an objective evaluating method for brainstem injury (author's transl)].

Far Field Acoustic Response (FAR) was repeatedly studied from acute stage to chronic stage of severe head injury in 64 patients for evaluation of the degree of brainstem injury. (1) In acute stage within 7 days after trauma, FAR abnormalities are found in 56% of the subjects with severe head injury. These abnormalities are as follows: (i) Prolongation of latency, disappearance of the 5th wave. (ii) Disappearance of the 1st-5th waves or 2nd-5th waves. The incidence of FAR abnormalities is not correlated to disease categories and age of the subjects. (2) FAR findings reveal more precisely the level of brainstem dysfunction than by clinical assessments of brainstem reflexes and posture, and by computerized tomography. (3) Evaluating results of the FAR recorded within 7 days after trauma are correlated to the outcome 3 or more months after injury in the subjects. Almost of them with alteration of only the 5th wave in FAR improve clinically and electrophysiologicaly at 2-3 weeks after trauma. However any patients with disappearance of the 1st-5th waves in FAR are fatal or fell into the state of vegetation. According to these results, it was concluded that FAR has a important diagnostic value as an objective evaluating method for brainstem dysfunction in patients with severe closed head injury.

Adolescent↗