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Biomedical subjects

H Nishida

Publications and source records attributed to H Nishida.

At least 487 records · Page 27Linked to original sources
PubMed1984

Immunohistochemical and electron microscopic observations of nevic corpuscle.

Nevic corpuscle in the pigmented nevus was studied under light microscopy in comparison with Meissner corpuscle of the human sole skin in order to determine its ontogenesis. No nerve fibers within nevic corpuscle but coiled fibers in Meissner corpuscle could be demonstrated by Bodian stain. Presence of S-100 protein in the pigmented nevus and Meissner corpuscle was observed under light microscopy utilizing peroxidase-antiperoxidase immunohistochemical staining. S-100 protein was more evident in nevic corpuscles than in Types A and B intradermal nevus cells. Meissner corpuscle showed moderate stainability for S-100 protein. Ultrastructural study revealed that the nevic corpuscle was composed of the laminated cells with flattened cytoplasmic processes but lacked peripheral nerve endings such as axons, characteristically shown in Meissner corpuscle. No melanogenic activity of nevic corpuscle was observed. These morphological and histochemical findings of nevic corpuscle did not enable us to give definite conclusion on its ontogenesis.

Female↗
PubMed1984

[Clinical studies on cefmenoxine concentration in prostatic tissue and bladder wall].

Clinical studies on cefmenoxime (CMX) concentration in prostatic tissue and bladder wall were made and the following results were obtained. In 40 patients undergoing operation, 1 g of CMX was administered intravenously by bolus technique and CMX levels in peripheral blood, prostatic tissue and bladder wall were examined. The maximum level of prostatic tissue was 35 micrograms/g after 0.41 hour and bladder wall was 62 micrograms/g after 0.37 hour, and T1/2 was 1.41 hours both. CMX, a new broad spectrum cephalosporin, can be considered as one of the highly useful antibiotics for the treatment of postoperative infection.

Cefmenoxime↗
PubMed1984

[Immunocytochemical localization of carcinoembryonic antigen (CEA) and transport of CEA into the blood vessels in gastric cancer].

CEA was localized in the luminal border, cytoplasm, but not in mucus, in Signet ring cell carcinoma (sig) and mucinous carcinoma (muc). Electronmicroscopically, CEA was localized in the glycocalyx of the microvilli and microvesicles of the cytoplasm. The histologically different cancer types showed no difference in the localization of T-CEA. We also studied P-CEA elevating factors in 38 CEA-positive (++) patients manifesting subserosal (ss) or deeper invasion. No remarkable findings were obtained. When P-CEA elevating factors were studied in 20 patients with (+) CEA reaction and ss or deeper invasion, we found that the incidence of por was high in P-CEA negative cases. In particular, the por incidence was significantly low (p less than 0.01) in patients with scirrhous type. High P-CEA levels were detected in blood adjacent to the cancer. Among 6 cases with negative P-CEA in the vessels adjacent to the cancer whose T-CEA reactions were (++) or (+), and who manifested ss or deeper invasion, there was a high incidence of por (5 cases) and scirrhous type (4 cases), histologically.

Adenocarcinoma↗
PubMed1984

[Effect of electroacupuncture on the sympathetic nervous, endocrine and metabolic functions].

Electroacupuncture (1.5 volts, 0.13 msec duration, 5 Hz for 10 min) was applied to the ten sites of lumbodorsal region in women aged 20 to 47. Immediately after the acupuncture plasma noradrenalin concentration significantly increased 9 out of 20 subjects, while it decreased in 11. Plasma noradrenaline returned to the intitial level in both groups 30 min after the acupuncture. In the noradrenaline elevated group plasma insulin concentration and plasma insulin/glucagon molar ratio significantly decreased until 30 min after the acupuncture. Such changes were not observed in the noradrenaline decreased group. Plasma glucagon, dopamine-beta-hydroxylase, free fatty acids, glycerol, creatine kinase and blood glucose were not changed in both groups.

Acupuncture Therapy↗
PubMed1983

Biological effects of Spirometra erinacei plerocercoids in several species of rodents.

Observations were made of the biological effects on infection with plerocercoids of Spirometra erinacei on normal female Snell mice, male chinese hamsters, golden hamsters, normal and hypox rats. Plerocercoid infection caused the strongest growth-promoting effect on normal Snell mice. In mice, this effect appears to be independent of strain. Chinese hamsters infected with these larvae showed similar growth. The infected normal rats and golden hamsters, however, showed a weight increase in the skeletal muscle only, while the hypox rats exhibited no effect at all. The elevation in the concentration of serum triglyceride was observed in all the animals investigated except for rats. Golden hamsters, in particular, exhibited a marked increase in the concentration of serum free fatty acids and total cholesterol. There was close correlation between the concentrations of serum triglyceride and free fatty acids, and the regression coefficient of the resulting linear regression equation for the experimentals was higher than that for the controls. This suggests that serum triglyceride results from an increased concentration of serum free fatty acids derived from stimulated lipolysis. The total cholesterol concentration in the serum decreased in chinese hamsters infected with larvae. The serum glucose concentration increased in normal Snell mice but decreased in chinese and golden hamsters. No difference in glycerol and free fatty acid concentration was observed in infected animals except for golden hamsters.

Animals↗
PubMed1983

Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. I. Up to the eight-cell stage.

Cell lineages during development of ascidian embryos were analyzed by injection of horseradish peroxidase as a tracer enzyme into identified cells at the one-, two-, four-, and eight-cell stages of the ascidians, Halocynthia roretzi, Ciona intestinalis, and Ascidia ahodori. Identical results were obtained with eggs of the three different species examined. The first cleavage furrow coincided with the bilateral symmetry plane of the embryo. The second furrow did not always divided the embryo into anterior and posterior halves as each of the anterior and posterior cell pairs gave rise to different tissues according to their destinies, which became more definitive in the cell pairs at the eight-cell stage. Of the blastomeres constituting the eight-cell stage embryo, the a4.2 pair (the anterior animal blastomeres) differentiated into epidermis, brain, and presumably sense organ and palps. Every descendant cell of the b4.2 pair (the posterior animal blastomeres) has been thought to become epidermis; however, the horseradish peroxidase injection probe revealed that the b4.2 pair gave rise to not only epidermis but also muscle cells at the caudal tip region of the developing tailbud-stage embryos. The A4.1 pair (the anterior vegetal blastomeres) developed into endoderm, notochord, brain stem, spinal cord, and also muscle cells next the caudal tip muscle cells. From the B4.1 pair (the posterior vegetal blastomeres) originated muscle cells of the anterior and middle parts of the tail, mesenchyme, endoderm, endodermal strand, and also notochord at the caudal tip region. These results clearly demonstrate that muscle cells are derived not only from the B4.1 pair, as has hitherto been believed, but also from both the b4.2 and A4.1 pairs.

Animals↗
PubMed1983

[Immunohistochemical localization of carcinoembryonic antigen (CEA) in gastric cancer--a comparative study between tissue CEA and plasma CEA].

Plasma carcinoembryonic antigen (CEA) was examined in 122 cases of gastric cancer and immunohistochemical localization was also studied in 102 resected specimens. A comparative study between tissue CEA and plasma CEA was carried out. The results were as follows. Plasma CEA were positive (over 2.5 ng/ml) in 46 cases (37%). The positive rate was parallel with the size of cancer (p less than 0.05) and was higher in well-differentiated cancer. As to tissue CEA, 87 specimens (85%) showed the varying degree (+/- +) of positive reaction. CEA was localized in luminal border, cytoplasma and grandular lumen in well differentiated cancer, and only in cytoplasma in poorly differentiated cancer. The positive rate of plasma CEA was parallel with the degree of positive reaction in tissue. No significant histological difference was observed between strong positive reaction to tissue CEA and positive plasma CEA. In the cases with moderate positive reaction to tissue CEA, however, the positivity of plasma CEA was significantly higher in the cases with alpha or beta INF pattern and/or intermed stromal reaction. (p less than 0.05).

Adenocarcinoma↗