Search PubMed⌕ Search

Biomedical subjects

H Naujoks

Publications and source records attributed to H Naujoks.

At least 37 records · Page 2Linked to original sources
PubMed1983

In vitro cultivation of xenotransplanted human breast-cancer cells.

Xenotransplanted human breast-cancer tissue growing in the second to the ninth passage in nu/nu mice and normal tissue from nu/nu mice was cultivated in vitro. Thirty-two of fifty-two (62%) cultures grew successfully and have been analyzed. The cells were observed using phase-contrast microscopy and May-Grünwald-Giemsa staining. The following cell types could be distinguished: normal fibroblast-like cells (FC), normal epithelial-like cells (EP I), abnormal epithelial-like cells (EP II), and degenerated or dying cells (DC). After staining, the nuclear area of all cell types was measured. The results were: FC = 280 micrometers 2, EP I = 320 micrometers 2, and EP II = 800 micrometers 2. They were compared with the nuclear area of human breast-cancer cells obtained by aspiration biopsy.

Animals↗
PubMed1979

[Preoperative evaluation by colposcopy and cytology in cervical intraepithelial neoplasia (cin) (author's transl)].

Before conization in 108 patients with cervical intraepithelial neoplasia (CIN) colposcopic findings and the results of triple cervical smears (anterior and posterior cervical lip and endocervical canal) have been evaluated and compared with the microscopic findings, limiting the comparison to the separately investigated anterior and posterior cervical lips. Colposcopically and cytologically suspicious findings agreed with the microscopic findings of CIN in 48.3% (anterior lip) resp. 41.7% (posterior lip). Cytologically suspicious findings alone have been determined in 80.5% resp. 84.8%; colposcopically suspicious findings were found in 55.2% resp. 48.1%. The main causes of false negative smears may have been failures of sampling, of false negative colposcopical findings a wrong estimation of an atypical transformation zone. A combined application of colposcopy and triple cervical smear technique in patients with abnormal smears is strongly recommended. This may improve the results of localizing a lesion of CIN.

Adult↗
PubMed1978

In vitro studies of human breast carcinoma cells obtained by aspiration biopsy.

Cells from 14 human breast carcinoma were obtained by aspiration biopsy and used for in vitro cultivation. Growth could be observed in three cultures, which were derived from solid, partly adenoid growing tumors with metastases in the axillary nodes. The growth started after 2--6 days in culture and lasted 2--4 weeks. No permanent line could be established.

Adenocarcinoma↗
PubMed1976

[Negative smear in cervical carcinoma in situ (author's transl)].

In 100 patients which had shown ca-in-situ in the conization specimen during a preoperative colposcopic and cytologic investigation separate smears were taken from the anterior lip, the posterior lip and from the cervical canal. The three slides in each case have been examined to determine the frequency of negative smears, especially those of the endocervix, and to evaluate any relationship between those findings and the localization and the extent of the lesion. 22% of these patients belonged to the age group of 20-29 years, 3% were older than 60 years. Depending on the microscopic description we differentiated between small, circumscript lesions (17 cases), intracervical lesions (24) and extended lesions (59). In only 49% of the cases all three smears had been positive (Pap III-V). In 23% of the cases the endocervical smear was negative. In small, circumscript lesions at least one negative smear was found in 80% of the cases. In the extended lesions one third had at least one negative slide. In 25% of the patients with an intracervical lesion the endocervical smear was negative. Re-evaluation of the negative smears has shown an examiner's failure in about one third of these, due to several factors. In 24 patients the triple cervical smear technique had been applied at least twice. One third of the slides exhibited differences concerning negativity and positivity between the first and the second preparation.

Adult↗
PubMed1976

[Hierdence of X-chromatin post partum].

Determinations of the X-chromatin frequency in buccal smears were performed in 33 pregnant women at the day before delivery and daily for seven days thereafter. In 23 women with a spontaneous delivery there was a statistically significant drop in the X-chromatin frequency after delivery being lowest on the second day. On the fifth day the X-chromatin frequency had reached the predelivery level. In 10 women with operative delivery by cesarean section and hormonal suppression of lactation no such changes in the X-chromatin-frequency have been noted.

Analysis of Variance↗
PubMed1975

[Early identification of cancer through vaginal cytology in a venereal disease clinic].

100 women, routinely and regularly examined in a venereal disease clinic, have been investigated using Papanicolaou smears of the uterine cervix for. 1. Papanicolaou group, 2. trichomonas infection, 3. assessment of vaginal microbiology. A group III was determined in 11, a group IV/V in 2 women. Trichomonas vaginalis was present in 60% of the cases. Only 9% of the smears showed normal Döderlein bacteria; 91% contained mixed bacteria. The findings are compared with a control group and are discussed in the light of epidemiologic studies indicating the close rôle of certain factors in the sexual life for the development of cervical cancer. The necessity to regard this population as a high risk group in general cytological screening has been stressed, especially to the advantage of the adolescent and young females in this group.

Adolescent↗