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Biomedical subjects

H Natori

Publications and source records attributed to H Natori.

At least 55 records · Page 3Linked to original sources
PubMed1987

Chromatographic analysis of human erythrocyte pyrimidine 5'-nucleotidase from five patients with pyrimidine 5'-nucleotidase deficiency.

Human erythrocyte pyrimidine 5'-nucleotidase (P5N) was separated into two subclasses. P5N-I and P5N-II, by DEAE Bio-Gel A column chromatography. Their enzymological properties were studied using five normal subjects and five patients with different P5N deficiencies. Study of the normal subjects showed that P5N-I and P5N-II have distinctive properties, and P5N-II is similar to the 5'-nucleotidase in rat liver cytosol. The P5N-II from the five subjects with this deficiency had normal activity and other normal enzymological properties. However, the P5N-I from these patients had abnormal properties, including reduced activity. These variant enzymes had a high Michaelis constant for substrate cytidine 5'-monophosphate and were heat stable. The optimum pH was shifted towards the acidic side in two patients, towards the basic side in one, and was unchanged in the other two. These results strongly suggest that the main cause of P5N deficiency is an abnormality of P5N-I, probably arising from a structural gene mutation.

5'-Nucleotidase↗
PubMed1986

Differentiation of primary or metastatic lung carcinoma by phospholipid analysis. A new approach for lung carcinoma differentiation.

Phospholipid analyses were performed on lung adenocarcinoma tissues from three patients who had undergone thoracotomy with a previous malignancy of the rectum or colon. The carcinomas were histologically difficult to differentiate as being primary or metastatic. The phospholipid profiles of the lung carcinomas in these cases were significantly different from those of primary lung adenocarcinoma, but were very similar to those of primary adenocarcinomas of the rectum or colon, suggesting that these carcinomas are metastatic. Therefore, the findings support the idea that phospholipid analysis of a tumor occurring in the lung could be very useful for differentiating whether such a tumor is primary or metastatic. The value of phospholipid analysis in assessing such tumors is emphasized as a new approach for differentiating lung carcinoma.

Adenocarcinoma↗
PubMed1985

Carcinoembryonic antigen in bronchoalveolar lavage fluid in patients with idiopathic pulmonary fibrosis.

An increased incidence of lung cancer and epithelial metaplasia or hyperplasia which is felt to be as a precursor of cancer, has been reported in patients with idiopathic pulmonary fibrosis (IPF). In this study, carcinoembryonic antigen (CEA) in bronchoalveolar lavage (BAL) fluid was measured in 53 control patients, 31 patients with sarcoidosis, 10 patients with hypersensitivity pneumonitis, 16 patients with primary lung cancer and 26 patients with histologically confirmed IPF. High ratio of CEA to albumin (Alb), exceeding mean + 2SD of nonsmoking control patients, were found in 8 (25%) out of 32 smoking control patients, 4 (44%) out of 9 nonsmoking patients with IPF, 8 (62%) out of 13 smoking patients with IPF, 3 (75%) out of 4 smoking patients with IPF and lung cancer and 13 (81%) out of 16 patients with primary lung cancer, although BAL was performed at the noncancerous parts of the lung in the cases of lung cancer. Furthermore, it was confirmed that CEA increased in BAL fluid in these subjects were different from nonspecific cross-reacting antigen (NCA) which was detectable in the normal lung. Thus we consider that the increase of CEA/Alb ratio in BAL fluid is a possible marker of these early histological disorders in the lung, and also suggests a greater risk of malignant change in the clinical course of IPF.

Bronchi↗
PubMed1985

Abnormality of helper/suppressor T cell ratio in patients with hemophilia.

Lymphocyte subpopulations in patients with hemophilia were analyzed. The results were compared with those in age- and sex-matched controls. The patients had been receiving blood and blood products including Factor VIII or Factor IX for a number of years as required at the time of the onset of the disease. Heparinized peripheral blood was used in direct tests for cell marker analysis with OKT4, OKT8 and OKT11 monoclonal antibodies. There were less OKT4+ cells in the hemophiliac blood as compared with the controls, while both the groups had the same number of OKT8+ cells. As a result, the OKT4/OKT8 ratio was markedly depressed in the hemophiliac group and the conversion of the OKT4/OKT8 ratio was observed in the group of patients receiving long-term therapy with anti-hemophiliac products. Alteration of lymphocyte subpopulations in patients with hemophilia in relation to antihemophiliac therapy was discussed.

Acquired Immunodeficiency Syndrome↗