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Biomedical subjects

H Nashimoto

Publications and source records attributed to H Nashimoto.

35 records · Page 2Linked to original sources
PubMed1979

Cloning of an EcoRI fragment carrying E. coli tufA gene.

EcoRI fragments of the transducing phage lambda fus3 DNA have been linked to the ColEl derivative plasmid RSF2124 (ColEl-Apr) DNA using bacteriophage T4 ligase. Among the plasmids formed, one designated pTUAl was found to contain the E. coli tufA gene. The proof for the presence of tufA gene in pTUAl is based on the following observations: (1) ability of pTUAl DNA and is EcoRI fragments to direct synthesis of EF-Tu in a cell-free protein synthesizing system; and (2) RNA . DNA hybridization of RNA transcribed from phage lambda rifd18 carrying tufB with DNA from pTUAl.

Coliphages↗
PubMed1970

Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits.

Some spectinomycin-resistant mutants of Escherichia coli are cold-sensitive. They are unable to assemble both 30S and 50S ribosomal subunits at low temperatures. They accumulate two kinds of incomplete particles, related to 30S and 50S subunits respectively. A single mutation, causing an alteration in a 30S ribosomal component, is responsible for these phenotypes. These results show that assembly of 50S subunits in vivo is dependent on simultaneous assembly of 30S subunits. On the other hand, the assembly of 30S subunits appears to be independent of 50S assembly.

Bacterial Proteins↗
PubMed1969

Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.

E. coli mutants that fail to grow at 20 degrees C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S and 32S particles, respectively. The third mutant produces reduced amounts of 50S ribosomes and accumulates 21S particles at low temperatures. The 43S and 32S particles contain 23S RNA and appear to be precursors of 50S ribosomes. The 21S particles contain 16S RNA. The mutation that causes accumulation of 32S particles is linked to the spc locus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study of in vivo ribosomal assembly.

Centrifugation, Density Gradient↗