[Malignant lymphoma of the uterine cervix].
A case of primary malignant lymphoma of the uterine cervix is presented followed by discussion of the problems in differential diagnosis and the potential curability of the condition.
Adult↗
Biomedical subjects
H Mygind
Publications and source records attributed to H Mygind.
[Parathyroid cancer. A review and 4 case reports].
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Adult↗
Acyclovir crystalluria.
Acyclovir crystals in the urine of a 28-year-old woman are described. The patient received intravenous acyclovir for herpes encephalitis. The crystalluria was accompanied by impairment of the renal tubules as evidenced by increased urinary beta-2-microglobulin excretion. Consecutive series of 60 patients on oral acyclovir and 20 patients on intravenous acyclovir were studied in order to define the frequency of acyclovir crystalluria. None of the patients studied had crystalluria, and none elevated serum creatinine levels. It is suggested that acyclovir crystalluria might be an early sign of acyclovir crystal nephropathy.
Acyclovir↗
Antikeratin antibodies in routine diagnostic pathology. A comparison of 10 different commercial antikeratins.
Ten commercially available antikeratin antisera were tested immunohistochemically on fresh frozen and formalin-fixed paraffin-embedded tissue. Eight of the antisera were in addition tested on protein-immunoblottings. For six of the antisera a good correspondence was found between our immunoblots and data given by the manufacturers. Two monoclonal antisera did not react with keratin proteins. On immunohistochemical testing two of the antibodies showed qualitatively identical staining on both frozen and paraffin sections without background staining. Three of the antibodies reacted weakly or not at all on paraffin sections but gave acceptable staining on frozen sections. Three of the antibodies showed acceptable staining on paraffin sections, but background staining on frozen sections and one antibody gave the reverse staining pattern. For one of the antibodies it was impossible to obtain an acceptable staining due to high non-specific binding of the secondary antibody. None of the antikeratins were true panepithelial tumour markers as all of them failed to detect keratin in at least one of the epithelial tumours. However, a combination of two or three antikeratins (Hybritech AE1 + AE3, Becton Dickinson No 7650, DAKO A622) covered most or all epithelial tumours examined. It is concluded that commercially available antisera show great variability with respect to quality and reactivity indicating that the majority need further purification, characterization and testing on tissues before they are introduced on the commercial market.
Antibodies↗
Prostatic carcinoma reproducibility of histologic grading.
The inter- and intraobserver variations of the histologic grading systems of prostatic carcinoma proposed by Gleason and by Böcking et al. were tested by the authors. Both grading systems have previously been shown to have good correlation with prognosis. After studying 91 cases of prostatic carcinomas, the interobserver agreements of the Gleason pattern score and the Böcking combined grade were found to be 36% and 69% respectively. Both observers regraded 31 randomly selected prostatic carcinomas. With the Gleason pattern score intraobserver agreements of 65% and 42% were found, whereas the intraobserver agreements of the Böcking combined grade were 90% and 71% respectively. Since the percentages of inter- and intraobserver agreements were higher with the system of Böcking et al., this system is recommended prior to the system of Gleason as a means of evaluating the prognosis of patients with prostatic carcinoma.
Humans↗
Influence of prefixation and fixation times on cellular preservation and epithelial cellularity in filter imprints.
Cells from malignant and nonmalignant lesions of the breast were suspended in three different fixatives or in a balanced electrolyte solution (Hank's), stored for varying periods of time, collected on Millipore filters and then imprinted on to clean microslides in order to evaluate the influence of prefixation and fixation time on epithelial cellularity and cellular preservation. The use of a methanol-acetic acid fixative (Esposti's fixative) or 50% isopropanol resulted in good preservation whereas cells prefixed in formaldehyde or 100% isopropanol were poorly preserved. Cells that had not been prefixed (suspended in Hank's solution) showed fair preservation. Eighty-eight percent of the imprints prepared from suspensions of Esposti's fixative were highly cellular, which was significantly better than with Hank's solution (68%), 50% isopropanol (66%), 100% isopropanol (56%) and formaldehyde (33%). The cellularity of the formaldehyde-prefixed imprints differed significantly from the others. There was no influence of storage time on either cellular preservation or epithelial cellularity for any of the investigated solutions.
Breast Neoplasms↗
Value of the filter imprint technique in aspiration biopsy cytology.
In 468 fine needle aspiration (FNA) biopsies, after smearing of the aspirate on glass slides, additional material was obtained by flushing the needle with a fixative. The cells were collected on Millipore filters, from which imprints were prepared. The filter imprints were found to be sufficiently cellular in 60% of the cases, which reduced by 33 (7.3%) the number of cases with unsatisfactory aspirates. Diagnostic specificity and sensitivity were not influenced by the technique. The filter imprint technique was of particular value in breast aspirates.
Biopsy, Needle↗