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Biomedical subjects

H Murakami

Publications and source records attributed to H Murakami.

At least 505 records · Page 28Linked to original sources
PubMed1992

Ten-year survivors with multiple myeloma.

Of 130 Japanese patients with symptomatic multiple myeloma who were treated between 1970 and 1989, nine (6.9%) patients survived for more than 10 years. Younger age, low and intermediate tumour mass, chemotherapy with cyclophosphamide, the disappearance of myeloma protein, and a positive response to retreatment were correlated with long-term survival.

Aged↗
PubMed1992

Ki-1-positive large-cell anaplastic lymphoma with protean manifestations including central nervous system involvement.

A 26-year-old female with Ki-1-positive large-cell anaplastic lymphoma is reported. The neoplastic cells were phenotypically and genotypically of T cell origin. Initially, neoplastic cells invaded the skin and lymph nodes, and then invaded the sternal and vertebral bones, ribs and the iliopsoas muscle. Central nervous system (CNS) involvement with paraplegia, large tumor mass formation in her breast and pleural infiltration ensued in succession. This case illustrates the protean manifestations of Ki-1-positive large cell anaplastic lymphoma complicated with CNS involvement.

Adult↗
PubMed1992

Prognostic relevance of morphological classification in multiple myeloma.

One hundred and twenty-two patients with multiple myeloma were classified as mature, intermediate, immature, or plasmablastic subtype according to Greipp's criteria. Contrary to Greipp's report, the survival time of plasmablastic myeloma was not significantly shorter than other subtypes, nor was the plasmablastic subtype identified as a poor prognostic factor. The survival time of mature plus intermediate myeloma was significantly longer than that of immature plus plasmablastic myeloma. Between the former and latter, significant differences were found for sex, clinical stage, thrombocytopenia, bone marrow plasmacytosis, renal insufficiency, bone destruction, and response rate to treatment. Therefore, it was suspected that the immature and plasmablastic subtypes were unfavorable prognostic factors in patients with multiple myeloma.

Aged↗
PubMed1992

Agranulocytosis during treatment with fluconazole.

Fluconazole, a new effective triazole antifungal agent, has been reported to cause fewer adverse reactions than amphotericin B. A patient who was diagnosed as having agranulocytosis and thrombocytopenia after treatment with fluconazole was investigated and recovered after withdrawal of the antifungal therapy. This case suggests the need for careful haematological observation during the treatment with fluconazole.

Adult↗
PubMed1992

[Antimutagenic activities of hexane extracts of the fruit extract and the kernels of Prunus mume Sieb. et Zucc].

Antimutagenic activities of hexane extracts obtained from the fruit extract (UE) and the Kernels (KE) of P. mume were examined. These extracts showed inhibitory activities to known mutagens, 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, benzo[alpha]pyrene and aflatoxin B1 in the Ames assay using Salmonella typhimurium TA100 and TA98 strains. The UE and KE fractions were then separated by silicic acid column chromatography with a stepwise elution method using ether-hexane. The location of the active substances in the fractions was also determined by thin-layer chromatography. Consequently, it was found that the effective substances for the desmutagenicity were fatty acids, and identified by gas liquid chromatography, mainly as oleic acid, linoleic acid and linolenic acid in UE, and mainly as oleic acid and linoleic acid in KE, respectively.

Antimutagenic Agents↗
PubMed1992

Stimulation of IgM production in human-human hybridoma HB4C5 cells by chitosan.

We screened for immunoglobulin production stimulating factors (IPSFs) in polysaccharides using human-human hybridoma cells, HB4C5, cultured in serum-free medium. Among polysaccharides, citrus pectin, locust bean gum, and chitosan stimulated IgM production of HB4C5 cells. Especially chitosan showed the strongest IPSF activity; 100 ng/ml of chitosan stimulated IgM production approximately 5-fold. Chitosan had several characteristics as IPSF, as follows. 1) For the IPSF activity, 70-90% deacetylation was essential. 2) Chitosan oligomers (n = 5, 6, 7) and chitin oligomers (n = 5, 6, 7) showed no IPSF activities. 3) The IPSF activity of chitosan was inhibited by glucosamine, one of the constitutive sugars of chitosan. 4) Chitosan stimulated IgM production of human lymphocytes in serum-free culture, but not IgG or IgA, nor in serum-supplemented culture.

Cell Line↗
PubMed1992

[Chronic lymphocytic leukemia diagnosed after corticosteroid therapy for Evans syndrome].

A 59-year-old woman was admitted in February 1991, because of abdominal distension. On admission, she had splenomegaly, autoimmune hemolytic anemia, and idiopathic thrombocytopenic purpura. Evans syndrome had been diagnosed and daily prednisolone therapy had been performed. After this therapy, a rapid increase of lymphocytes was observed accompanied with contraction of spleen. The monoclonal proliferation of B-lymphocytes and rearrangement of JH and J kappa gene were detected and chronic lymphocytic leukemia was diagnosed. With reduction of prednisolone, the lymphocyte counts decreased and the size of the spleen returned to the previous state. It is suspected that the cause of rapid lymphocytosis in this case was due to the redistribution of lymphocytes from the spleen to the peripheral blood.

Anemia, Hemolytic↗
PubMed1992

A long-term survivor with malignant thymoma accompanied with pure red cell aplasia.

A 56-year-old woman with malignant thymoma lived for 19 years after diagnosis and died of superior vena cava syndrome. An autopsy showed invasion of the thymoma to the right lung and metastasis to the left lung. The pathologic diagnosis was epithelial thymoma (spindle cell dominant) accompanied with pure red cell aplasia (PRCA). However, this was well controlled with steroid therapy. She was the longest survivor with malignant thymoma at this time. Her long-term survival may be the result of good control of the PRCA.

Female↗
PubMed1992

[Long-term results of terminal esophago-proximal gastrectomy for esophageal varices].

Between 1973 and 1991, 193 patients underwent terminal esophagoproximal gastrectomy (TEPG) for esophageal varices. One hundred and sixty patients (84%) were cirrhotics. Ten patients (5.2%) were died within hospital stay. In 116 elective patients who had been free from hepatocellular carcinoma during therapeutic courses, the 5- and 10-year survival rates were 77% and 62% in Child A, and 62% and 38% in Child B, respectively. There was a significant difference between the two groups (p < 0.05). Postoperative rebleeding from esophageal varices was infrequent within 5 years but increased after 5 years especially in Child B and Child C. For patients with these recurrent varices, endoscopic injection sclerotherapy was very effective and improved the prognosis. The survival rate of patients with extremely decreased platelet counts was not different from that of patients without. There were no other severe complications after TEPG. We conclude that TEPG would be indicated firstly for elective or prophylactic cases unless they have severe hepatic damage.

Esophageal and Gastric Varices↗
PubMed1992

Functional electrical stimulation (FES) systems for restoration of motor function of paralyzed muscles--versatile systems and a portable system.

Multichannel functional electrical stimulation (FES) systems were developed to restore the motor functions of the paralyzed muscles, i.e. two kinds of versatile systems for laboratory use and a practical portable system were developed. The first microcomputer-based FES system was composed of an 8 bit microcomputer (PC-8801mkII) with a voice recognition board and 30 stimulation outputs, in which the voice commands were used for selecting a motion pattern, and for executing (start), (hold), (restart) and (stop) commands. In combination with the voice commands, a proportional control command detected from a head angle sensor was used for volitional control of motion. The second microcomputer-based FES system was composed of a 16 bit microcomputer (PC-9801 UV or compatible) with flexible control capabilities (e.g. respiratory sensors) for volitional control and with 64 stimulation outputs. An originally designed practical portable FES system was 89 x 145 x 31 mm in dimension and 380 g in weight. This paper describes the system configurations, the control methods of these FES systems and three examples of the clinical applications of the FES systems.

Electric Stimulation Therapy↗
PubMed1992

Hematopoietic progenitor cells in a patient with myeloid metaplasia without myelofibrosis.

A rare case of myeloid metaplasia without myelofibrosis is presented. Findings that the increase in blood megakaryocyte progenitor cells was not as prominent as the increase in granulocyte-monocyte and erythroid progenitor cells, and that the increase in bone marrow megakaryocytes was only slight might explain the absence of bone marrow fibrosis in this case. However, it is not clear whether the disease is a subtype or merely an early stage of myelofibrosis with myeloid metaplasia.

Aged↗
PubMed1992

Growth and utilization of nutrients in newly-hatched chick with or without removal of residual yolk.

Two experiments were conducted to characterize the growth and utilization of nutrients in newly-hatched chicks, and to determine nutritional roles of residual yolk by comparing chicks with or without deutectomy (removal of residual yolk sac). Rapid growth during 14 days after hatch was shown in the fed chicks. Daily feed intake increased linearly for 15 days posthatch, resulting in a high efficiency (80%) of feed utilization. Residual yolk in the abdomen averaged 6.4 g at hatch and rapidly disappeared during the first 3 days. Posthatch starvation resulted in a decrease in carcass lipid content but did not modify the disappearance rate of yolk in the abdomen. Weight of the digestive tract per unit body weight increased markedly up to 3-7 days of age followed by a gradual decrease in the fed chicks. Metabolizability of dietary energy and absorption of dietary lipid were the highest at hatch, then declined to the lowest at day 5 or 6, and thereafter gradually increased. The deutectomy did not influence the metabolizability of dietary energy and lipid, and the carcass composition, but delayed the growth by 2 days behind the control chicks. These results indicate that the metabolism and nutrition of newly-hatched chicks markedly fluctuate and that the residual yolk has crucial roles in complementing the nutrients absorbed to assure their rapid growth posthatch.

Animal Nutritional Physiological Phenomena↗
PubMed1992

[Distribution of IGG antibodies to several phospholipids in patients with pulmonary thromboembolism].

Seven pulmonary thromboembolism cases with IgG antibodies to three phospholipids i.e. cardiolipin (CAR), phosphatidyl inositol(PI) or phosphatidyl serine(PS) were reported. We investigated the distribution of these antibodies in relation to clinical courses or clinical laboratory data. The cases had anti-phospholipid antibodies not only to CAR but also to PI or PS without regard to collagen disease cases or non-collagen disease cases. Two out of three collagen disease cases were positive to 2 antibodies among the measured 3 IgG antibodies. The two cases were chronic progressive, complicated with multiple thromboses, prolongation of blood coagulation time and decrease of platelet count. The other four non-collagen disease cases were positive to only 1 antibody among the 3 antibodies. Most of the 4 cases were acute and there were cases not complicated with multiple thromboses, prolongation of blood coagulation time or decrease of platelet count.

Adult↗
PubMed1992

In vitro immunization of human lymphocytes with human lung cancer cell line A549.

We established a method of in vitro immunization of human lymphocytes against human cancer cells and efficiently obtained human-human hybridomas producing cancer-specific antibodies using the in vitro immunized lymphocytes. Human lymphocytes were cocultured with human lung cancer cell line A549 for four days in medium containing various immunoactive reagents. In vitro immunization was effectively caused by the addition of OK-432 or muramyl peptides, IL-2, and IL-6, to the coculture of A549 cells and lymphocytes. Although specific antibodies of both IgM and IgG classes were produced by this method, the ratio of IgG to IgM was greater than or equal to 2. The efficiencies of in vitro immunization fluctuated about threefold in lymphocytes derived from several donors. The in vitro immunized lymphocytes were fused with human fusion partner A4H12 cells. Hybridomas producing specific antibodies reactive to A549 cells were efficiently obtained by sequential cell fusion. The efficiency of acquisition of hybridomas producing specific antibodies with the in vitro immunized lymphocytes was about 10-fold higher than that with lymphocytes spontaneously immunized in bodies of lung cancer patients.

Acetylmuramyl-Alanyl-Isoglutamine↗