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Biomedical subjects

H Muller

Publications and source records attributed to H Muller.

At least 55 records · Page 3Linked to original sources
PubMed1982

[Kveim test performed with antigen obtained from lymph nodes].

This is our second paper about the Kveim test performed with G-77 antigen obtained from sarcoid lymph nodes. The antigen was tested in 46 patients with proved or suspected sarcoidosis and in 50 controls. Results were: 1) In 27 patients with active proved sarcoidosis and without treatment the test was positive in 85,1% (23/27 patients); 2) In 2/3 patients with proved sarcoidosis after therapeutic remission for more than two years and without treatment presently, the results were positive in one of them; 4) In 14 patients suspected to have sarcoidosis but showing lesions only on the skin the test was positive in 58,1% (8/14 patients); 5) In 48 controls the test was negative but in 2 it was positive. Our results agree with those of the literature.

Antigens↗
PubMed1981

[Value of morphine derivatives administered by the peridural route per- and postoperatively].

The existence of opiate receptors in the spinal cord led the authors to seek a clinical application. 1 - A peroperative injection of morphine was administered in 170 cases: 0.005 mg/kg of fentanyl in 105 cases and 0.05 mg/kg of morphine in 65 cases. In addition to usual surveillance (blood pressure, heart rate and central venous pressure), more extensive haemodynamic investigations were undertaken in 20 patients using a Swan-Ganz catheter. Blood concentrations (11 cases) and CSF concentrations (2 cases for each time of measurement) were determined in the case of fentanyl. In 20 patients (10 of whom had received fentanyl and 10 morphine) there was sophisticated cardio-respiratory surveillance postoperatively. 2 - 0.05 mg/kg or morphine (404 cases), 50 mg of pethidine (10 cases) and 0.1 mg of fentanyl (10 cases) were injected postoperatively. A comparison was made of the analgesia obtained. After three types of anaesthesia: epidural with bupivacaine with intubation (10 cases), halothane with intubation (10 cases) and neuroleptanaesthesia (10 cases), an injection was given of 0.05 mg/kg of morphine, with cardiorespiratory surveillance. Results were as follows: 1 - There were no significant variations in haemodynamic parameters peroperatively, indicative of adequate analgesia. Blood concentrations of fentanyl were as follows: 3.2 +/- 2.1 ng/ml after 10 minutes, 2 +/- 1.7 ng/ml after one hour, 1.4 +/- 1 ng/ml after two hours and 0.4 +/- 0.3 ng/ml after four hours. CSF concentrations were much higher; 34 ng/ml after one hour, 30 ng/ml after two and three hours and 25 ng/ml after four hours. No cardio-respiratory depression was seen after the peroperative injection of morphine. 2- The duration of analgesia following a postoperative injection of a morphine derivative was as follows: morphine 17.3 +/- 3.9 hours, pethidine 3.5 +/- 0.5 hours, and fentanyl 5.1 +/- 0.7 hours. The epidural injection of morphine after neuroleptoanaesthesia caused respiratory depression in two of the 10 cases, with a rise in pCO2 of 0.45 and 0.52 KPa. The results are discussed and compared with those of other authors. In conclusion, the authors emphasize the advantages of this method which makes it possible to obtain with smaller doses analgesia of longer duration than following a systemic injection of morphine, whilst at the same time decreasing the side effects.

Anesthesia, Epidural↗
PubMed1980

Studies on the association of NAD glycohydrolase with membranes in calf spleen.

The subcellular distribution of NAD glycohydrolase was studied by fractionation of calf spleen homogenates using differential and discontinuous density gradient centrifugations. The highest amount of NAD glycohydrolase activity was associated with microsomes, which in this tissue were found to contain, in addition to endoplasmic reticulum, a large proportion of vesicles derived from plasma membranes. The distribution pattern of NAD glycohydrolase was found to parallel that of plasma membrane markers. When microsomal vesicles were treated with digitonin, NAD glycohydrolase activity and plasma membranes specifically increased in density. We conclude that in calf spleen the bulk of NAD glycohydrolase is associated with plasma membranes. Microsomal NAD glycohydrolase was associated with sealed vesicles; its activity could not be increased by disruption of the sidedness of the vesicles. This result and further observations based on the known restricted permeability of biological membranes to charged substances, and on the activity of the enzyme with non-penetrating substrates and inhibitors, indicate that the NAD glycohydrolase active site is located on the exterior side of the vesicles. It is proposed that calf spleen NAD glycohydrolase is an ecto-enzyme.

Animals↗
PubMed1979

Calf spleen NAD glycohydrolase. Comparison of the catalytic properties of the membrane-bound and the hydrosoluble forms of the enzyme.

The catalytic properties of membrane-bound calf spleen NAD glycohydrolase were studied in comparison with previous data obtained with a solubilized hydrosoluble form of the enzyme. When the hydrolysis of NAD catalyzed by membrane-bound NAD glycohydrolase was studied at pH values below 7.5, only insignificant interference by other NAD-hydrolyzing enzymes was detected, and no proton-diffusional inhibition was observed. The kinetics could, therefore, be followed using a titrimetric assay for NAD glycohydrolase activity. The effect of pH, ionic strength on the kinetic parameters, and shifts in binding constants for several ligands of the membrane-bound enzyme indicate that the NAD glycohydrolase activity is influenced by an electrostatic potential due to negative charges (polyelectrolyte effect). No significant changes in kinetic mechanism could be found between both NAD glycohydrolase forms. The association of the enzyme with the membrane results in a remarkably increased thermal stability, in changes in binding properties of the active site and in the emergence of new inhibitor binding sites; e.g. adenosine 3':5'-monophosphate (cyclic AMP) and adenosine, which do not inhibit the hydrosoluble form of NAD glycohydrolase, are good inhibitors (respectively competitive and mixed) of the membrane-bound enzyme. These data (i.e. allotopic changes) probably can be ascribed to enzyme conformational changes induced and stabilized by interaction with membrane constituents.

Animals↗
PubMed1978

Ring 10 chromosome: 46,XX,r10(p15q26).

A teenage girl with mild retardation and some clinical features suggestive of the Turner syndrome was found to have an r10(p15q26) in blood and skin cells. Quantitative evaluation of 28 red cell enzymes, including two (hexokinase and glutamic-oxalocetic transaminase) known to be on chromosome 10, gave normal values.

Adolescent↗