Search PubMed⌕ Search

Biomedical subjects

H Mukoyama

Publications and source records attributed to H Mukoyama.

27 records · Page 2Linked to original sources
PubMed1990

Isolation of H and Leb active human salivary mucins and structures of their sugar chains with the H and Ley (Y) determinants.

Blood-group active mucins were prepared from an OLeb donor's saliva. Salivary proteins were carboxamidomethylated and fractioned on Sepharose CL-4B in the presence of sodium dodecyl sulfate. H and Leb active mucins were eluted at the void volume and were purified by Sepharose CL-2B chromatography. The purified mucins comprised 71% carbohydrate, 26% protein and 3.1% sulfate (by weight). Sugar chains of the mucins were released by alkaline borohydride treatment and fractionated by serial liquid chromatography. Three neutral oligosaccharides were isolated and submitted to sugar analysis and 1H-nuclear magnetic resonance spectroscopy. The following structures are proposed: Fuc alpha 1-2Gal beta 1-3[Fuc alpha 1-2Gal beta 1-4GlcNAc beta 1-6]GalNAcol, Fuc alpha 1-2Gal beta 1-3[Fuc alpha 1-2Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta 1-6]GalNAcol and Fuc alpha 1-2Gal beta 1-3GlcNAc beta 1-3GalNAcol, which contain the H type 2, Ley (Y) and H type 1 determinants, respectively. The present report provides the first identification of some antigenic structures in human blood-group active salivary mucins.

ABO Blood-Group System↗
PubMed1989

Determination of ABO blood groups from saliva and saliva stains by an indirect enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies.

The detection of A, B and H blood group substances (ABH-BGS) in saliva and in saliva stains has been investigated quantitatively by an indirect ELISA using a horseradish peroxidase conjugate in combination with the use of monoclonal antibodies. Through this method, the reaction specificity to BGS in the saliva was very high and its detection sensitivity was found to be approximately 1,000 times greater than has been achieved in a hemagglutination-inhibition test. The monoclonal anti-A and anti-B reagents reacting with both secretor and non-secretor saliva in a hemagglutination-inhibition test and in this ELISA method were selected from among commercial monoclonal antibodies. However, no monoclonal anti-H reagent was found to react with non-secretor saliva. The BGS level was determined by the use of calibration curves of A, B and H standard BGS from human gastric mucosa and was expressed in units, based on the inhibition titer of the standard BGS. In 230 saliva samples, ABH-BGS were detectable, except for H BGS in non-secretor saliva. The BGS levels in saliva stains experimentally prepared were found to be approximately proportional to the levels in the original saliva. As for actual and aged stains, it was possible to detect BGS in most cigarette butts and in aged stains, however, such detection proved impossible in saliva samples from postage stamp.

ABO Blood-Group System↗
PubMed1988

Arabinogalactan-Proteins from Primary and Mature Roots of Radish (Raphanus sativus L.).

Organ-specific variations in blood group H-like activity were observed in developing radish plants. A temporary increase in serological activity was found to occur in the roots at the earlier stages of development. Arabinogalactan-proteins (AGPs) were isolated from primary and mature roots, and investigated for changes in their physicochemical properties, structure, and serological activities. These root AGPs were composed mainly of l-arabinose and d-galactose but were distinguishable from each other in their contents of l-fucose as well as of protein and hydroxyproline. The structures of the carbohydrate moieties of the root AGPs were essentially similar to those of AGPs isolated from seeds and mature leaves in that they consisted of consecutive (1-->3)-linked beta-d-galactosyl backbone chains having side chains of (1-->6)-linked beta-d-galactosyl residues, to which alpha-l-arabinofuranosyl residues were attached in the outer regions. One prominent feature of the primary root AGPs was that they contained appreciable amounts of l-fucose, which was presumably responsible for expression of the serological activity. In their immunological reactions with rabbit anti-radish leaf AGP antibody, the root AGPs were shown to share common antigenic determinant(s) with those of seed and leaf AGPs.

Journal Article↗
PubMed1978

[Evolution of anti-Rh antibodies idiotypy in a blood donor over 9 years].

Anti-idiotypic sera were obtained in Rabbits immunized with Rh antibodies (isolated from the serum sample collected in 1974 from a Blood Donor). The sera agglutinate, at high titers, red cells coated with the immunizing antibodies and, at different titers, cells coated with antibodies from serum samples taken at other periods. Inhibition of hemagglutination of anti-idiotypic sera by different samples from the same Donor was complete with the immunizing serum and partial with other samples. These results show that idiotypes or idiotypic specificities appeared or disappeared during the period studied and represent the first observation on evolution of antibody idiotypy in Man.

Animals↗
PubMedDate not supplied

Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup.

The genetic relationships of seven Japanese and four mainland-Asian horse populations, as well as two European horse populations, were estimated using data for 20 microsatellite loci. Mongolian horses showed the highest average heterozygosities (0.75-0.77) in all populations. Phylogenetic analysis showed the existence of three distinct clusters supported by high bootstrap values: the European cluster (Anglo-Arab and thoroughbreds), the Hokkaido-Kiso cluster, and the Mongolian cluster. The relationships of these clusters were consistent with their geographical distributions. Basing our assumptions on the phylogenetic tree and the genetic variation of horse populations, we suggest that Japanese horses originated from Mongolian horses migrating through the Korean Peninsula. The genetic relationship of Japanese horses corresponded to their geographical distribution. Microsatellite polymorphism data were shown to be useful for estimating the genetic relationships between Japanese horses and Asian horses.

Alleles↗
PubMedDate not supplied

In vitro biotransformation of finasteride in rat hepatic microsomes. Isolation and characterization of metabolites.

Metabolism of finasteride ([N-(1,1-dimethylethyl)-3-oxo-4-aza-5 alpha-androst-1-ene-17 beta- carboxamide]; MK-906), a new type of specific inhibitor of testosterone 5 alpha-reductase, was investigated using rat hepatic microsomes. The metabolism of finasteride by rat hepatic microsomes was oxygen- and NADPH-dependent, and addition of metyrapone, 7,8-benzoflavone, and cytochrome c to the incubation mixture inhibited the metabolism of finasteride. It is suggested that the metabolic reaction of finasteride was mediated by a mixed function oxidase involving P-450. Four major metabolites were detected in vitro on incubating finasteride with hepatic microsomes of rats treated with phenobarbital (PB-Ms), whereas two major metabolites were found in the incubation mixture with microsomes of untreated rats (UT-Ms). These metabolites were isolated and purified by solvent extraction and semi-preparative HPLC, and identified by MS spectrometry and NMR spectroscopy. The metabolites consisted of omega-hydroxy finasteride (M-1), finasteride-omega-al (M-2), finasteride-omega-oic acid (M-3), and 6 alpha-OH finasteride (M-4). M-1 and M-4 are the major metabolites in UT-Ms, and M-1 and M-3 in PB-Ms. These studies revealed that hydroxylation of the t-butyl group and ring hydroxylation at the 6-position were key steps in the metabolism of finasteride in the rat hepatic microsomes. Further, the major metabolite M-4 was hydroxylated at the 6 alpha-position, but not at the 6 beta-position of the drug. This finding suggests the existence of a novel enzyme that catalyzes the 6 alpha-hydroxylation of the 4-azasteroid.

Animals↗