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Biomedical subjects

H Mohler

Publications and source records attributed to H Mohler.

62 records · Page 4Linked to original sources
PubMed1985

Glucose tolerance during chronic propranolol treatment.

Chronic diuretic therapy in hypertensive patients may be associated with a reversible deterioration in glucose tolerance. There is evidence to suggest that beta-blocker monotherapy may cause a deterioration in glucose tolerance in patients with non-insulin-dependent diabetes mellitus but little information about the effects of chronic beta-blocker treatment in hypertensive patients with normal glucose metabolism. A prospective evaluation of the effects of propranolol monotherapy on glucose tolerance was performed in 13 nondiabetic hypertensive patients. One patient developed diabetes mellitus after 1 month of treatment. Of 11 patients, 4 had a 30% or greater increase in fasting glucose after 1 year of therapy and a significant increase in fasting, and 2-hour postglucose-load venous plasma glucose occurred in the 9 patients completing 18 months of treatment. Although the biologic significance of these changes is questionable, the development of hyperglycemia in a patient receiving beta blockers could represent a side effect of drug therapy.

Adult↗
PubMedDate not supplied

Immunobiochemical characterization of the NMDA-receptor subunit NR1 in the developing and adult rat brain.

To investigate the developmental and regional expression of the NR1-subunit of the NMDA-receptor on the protein level, two polyclonal antisera [NR1(N) and NR1(C)] were raised against fusion proteins derived from the N- and C-terminal domain of the NR1-subunit, respectively. In Western blots of rat brain membranes, both antisera specifically recognized a single protein band with an apparent molecular size of 115 kDa. The regional distribution of the NR1-subunit immunoreactivity was analyzed in the developing and adult rat brain using sections blotted onto nitrocellulose membranes for immunostaining. With the NR1(N)-antiserum, strongest signals were detected in hippocampus, followed by cortex, striatum and thalamus, and weaker staining was observed in tectum, brainstem and cerebellum of adult brain. The NR1(C)-immunoreactivity exhibited a similar distribution, except that the staining in thalamus, tectum, brainstem and cerebellum was faint or virtually absent. The distinct pattern of NR1(N)- and NR1(C)-immunoreactivity arose during postnatal development. At birth, moderate staining with both NR1-subunit antisera was observed throughout the brain increasing strongly in most brain regions until postnatal day 21. In some brain areas, however, the NR1(C)-, in contrast to the NR1(N)-staining, decreased postnatally e.g. in thalamus, tectum and brainstem. The restricted staining intensity of the NR1(C)-antiserum in particular areas of adult and developing brain appears to reflect the emergence of C-terminal splice variants of the NR1-subunit which are not recognized by the NR1(C)-antiserum.

Animals↗
PubMedDate not supplied

Immunocytochemical localization of the GABAA/benzodiazepine receptor beta2/beta3 subunits in the optic tectum of the salmon.

The optic tectum of the salmon is a primary visual center with direct input from the retina via the optic tract. The structure is homologous with the superior colliculus of the mammalian brain. We have studied the distribution of immunoreactivity against the GABAA/benzodiazepine receptor beta2/beta3 subunits with a monoclonal antibody (BD-17) in the optic tectum of the salmon brain. A weak immunoreactivity is found in the rostral stratum marginale (SM), strong labelling of the neuropil is shown in a thin band in stratum opticum (SO), two bands in stratum fibrosum et griseum superficiale (SFGS) and two bands in stratum griseum centrale (SGC). Immunoreactive perikarya with neurites that extend radially through the stratum album centrale (SAC) are located in the stratum periventriculare. BD-17 immunoreactivity is to a great extent located in tectal layers that receive direct retinal input, i.e. the SO, SFGS and SGC. These layers are known to receive input also from other visual centers, such as the pretectum (SO, SFGS), the nucleus isthmi (SO, SFGS, SGC), as well as non-visual regions as the telencephalon (SGC). High levels of 2-[125I]-iodomelatonin binding sites have previously been demonstrated in all layers of the salmon optic tectum except the SM and SPV. Thus it appears likely that GABA and/or benzodiazepines and melatonin play a role in visual processing in the optic tectum of teleost fish.

Animals↗
PubMedDate not supplied

Genetic approaches to CNS disorders with particular reference to GABAA-receptor mutations.

The tools of molecular biology will bring the field of human genetics into a new era by permitting the analysis of the genetic contribution to disease. Most single gene disorders, inherited in a Mendelian fashion, will be molecularly diagnosed. In addition, the genetic susceptibility of common, complex diseases such a schizophrenia can be clarified, even though the conditions are not inherited as Mendelian characteristics. The mapping of the human genome will increase the rate at which new disease genes are identified and isolated. Finally, the development of genetically engineered animal models will help to dissect the steps involved in physiological and pathophysiological processes and thereby enhance our understanding of complex biological systems.

Alcoholism↗