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Biomedical subjects

H Mitsuya

Publications and source records attributed to H Mitsuya.

At least 253 records · Page 14Linked to original sources
PubMed1986

Human monoclonal antibody directed against an envelope glycoprotein of human T-cell leukemia virus type I.

We report the production and characterization of a human monoclonal antibody reactive against the major envelope glycoprotein of human T-cell leukemia virus type I (HTLV-I), a virus linked to the etiology of adult T-cell leukemia. We exposed lymph-node cells derived from a patient with adult T-cell leukemia to the Epstein-Barr virus in vitro and obtained a B-cell clone (designated 0.5 alpha) by a limiting dilution technique. The secreted product of 0.5 alpha is a monoclonal antibody (also designated 0.5 alpha; that is IgG1 and has kappa light chains) that binds to the cell membrane of T-cells infected with HTLV-I and lyses them in the presence of complement. The antibody does not react with HTLV-I-negative T cells. In electroblot assays, the monoclonal antibody detects a 46-kDa glycoprotein in disrupted HTLV-I virions and a 34-kDa product following digestion of the viral protein with endoglycosidase F. These molecules have been reported to represent the HTLV-I env gene products. The antibody does not react with HTLV-II and HTLV-III virions. Glycoproteins of 61 and 68 kDa, which are known to be encoded at least in part by the env gene of HTLV-I, are precipitated by the antibody from endogenously radiolabeled HTLV-I-infected HUT 102-B2 and MT-2 cells, respectively. These results suggest that this human monoclonal antibody reacts with an env-encoded glycoprotein of HTLV-I. By using a competition assay with a biotin-labeled 0.5 alpha antibody, we observed that 15 out of 15 patients with adult T-cell leukemia had antibodies that block binding of the 0.5 alpha antibody to HTLV-I virions. This suggests that the antigen detected by 0.5 alpha antibody is a common epitope recognized in HTLV-I-infected individuals in vivo. This antibody, as well as the general strategy for making human monoclonal antibodies reactive against pathogenic retroviruses, may have diagnostic or therapeutic application.

Antibodies, Monoclonal↗
PubMed1986

Labial adhesions in a diabetic woman.

This paper reports a 72-year-old woman with labial adhesions, who complained of difficulty in urination and terminal dribbling. The adhesions were surgically dissected with success. Diabetes mellitus in addition to poor perineal hygiene seem to have made her susceptible to the recurrent vulvitis which led to labial adhesions. This case implies the significance of detecting diabetes mellitus as a contributory factor for labial adhesions.

Aged↗
PubMed1986

Alterations in cytotoxic and helper T cell function after infection of T cell clones with human T cell leukemia virus, type I.

HTLV-I is a transforming human retrovirus that is an etiologic agent of adult T cell leukemia/lymphoma. To investigate the effects of this virus on T cell functions, two OKT3+, OKT4+, OKT8- cytotoxic clones (8.7 and 8.8) specific for allogeneic cells bearing DPw2, a class II histocompatibility antigen, were studied before and after infection with HTLV-I. The clones retained cytotoxic function for up to 70 d after exposure to HTLV-I, even without subsequent antigenic stimulation, but then lost their cytotoxic activity. Prior to infection with HTLV-I, clone 8.8 also lysed OKT3 hybridoma cells; after infection, cytotoxic activity against these OKT3-antibody bearing cells was lost in parallel with the loss of activity against DPw2-bearing target cells. In addition, expression of T3 surface antigen by HTLV-I-infected 8.8 cells was decreased at a time when they lost their cytotoxic activity, possibly contributing to the loss of cytotoxic function. Finally, clone 8.8 could provide help for nonspecific IgG production by autologous B cells when stimulated with irradiated DPw2-bearing non-T cells. After infection with HTLV-I, this helper function became independent of DPw2-stimulation and persisted even when the cytotoxic activity was lost. An OKT4+ T cell clone thus could simultaneously manifest both cytotoxic and helper T cell activities, and these activities were differentially affected after HTLV-I infection.

Antibodies, Monoclonal↗
PubMed1986

Infection of human T lymphotropic virus-I-specific immune T cell clones by human T lymphotropic virus-I.

Human T lymphotropic virus-I (HTLV-I)-specific T cell lines were established and cloned. K5, an OKT8+ clone bearing multiple proviral integration sites, retained its HTLV-I-specific cytotoxicity and a normal dependence on interleukin 2 (IL-2), indicating that there is a finite number of transforming integration sites. R2, an OKT4+ HTLV-I-infected clone, initially mounted a proliferative response to HTLV-I; but then its IL-2-independent proliferation increased and the antigen specificity was lost. All HTLV-I-infected clones tested including K7, another OKT8+ transformed cytotoxic clone that had lost its reactivity, expressed comparable levels of T cell receptor beta-chain (TCR-beta) messenger (m)RNA. Although clones K5 and K7 had different functional properties, they had the same rearrangement of the TCR-beta gene, suggesting that they had the same clonal origin. These data indicate that HTLV-I-specific T cells retain their immune reactivity for variable periods of time following infection, but then usually lose it; in some cases, however, no alteration in function can be detected. The data also suggest that different consequences can take place in the same clone depending on the pattern of retroviral infection.

Antibodies, Monoclonal↗
PubMed1986

Pharmacological and histological evidence for adrenergic innervation of the myoid cells in the rat seminiferous tubule.

Although the existence of seminiferous tubule contractions attributed to peritubular myoid cells is established, the control of the contractions is poorly understood. In this communication, it is shown that the rat seminiferous tubule responds to autonomic drugs and to stimulation of the perivascular nerve running along the spermatic vessels, by means of recording the intratubular pressure with a servo-null micropressure measuring device. Furthermore, the presence of nerve fibers close to the myoid cells is shown using a silver impregnation technique. Furthermore, the nature of the neurotransmitter contained in synaptic vesicles using 5-hydroxydopamine and L-DOPA as markers of adrenergic nerve elements with electron microscopy is shown in this report. It is concluded that there are adrenergic alpha- and beta-receptors and muscarinic receptors in myoid cells of the rat seminiferous tubule and that the contractions of seminiferous tubules are regulated by adrenergic nerve fibers.

Acetylcholine↗
PubMed1986

[Immunohistochemical localization of epithelial membrane antigen, carcinoembryonic antigen and secretory component in urinary bladder cancer].

To clarify the relationship between the immunohistochemical distribution pattern of epithelial antigens in transitional cell carcinomas and their histopathological grading and staging, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA) and secretory component (SC) were localized. Formalin-fixed paraffin-embedded sections from 55 patients with bladder carcinoma were stained by the indirect immunoperoxidase method. In normal transitional epithelium, EMA was found on the luminal side of the plasma membrane of a few surface layers, and in the cytoplasm of the superficial cells. In the lower grade and stage of transitional cell carcinoma, only the luminal surface of superficial cells was positively stained. Membrane and cytoplasmic staining of EMA was frequently found in the intermediate and basal layers of the carcinoma, and the incidence of cytoplasmic staining increased with the higher grade and stage. CEA was not detected in normal epithelium. Cytoplasmic staining of CEA was progressively more frequent in the higher grade and stage of transitional cell carcinoma. In normal epithelium SC was observed on the apical surface plasma membrane and in the cytoplasm of the superficial cells, as shown in the immunohistochemical staining for EMA. The correlation of immunohistochemical detection of SC with the grade or stage was not as good as the correlations for EMA or CEA. These findings suggest that immunohistochemical examination for EMA, CEA and SC in bladder carcinoma could provide valuable information for grading or staging in pathological diagnosis.

Carcinoembryonic Antigen↗
PubMed1986

Combined prostatectomy and retropubic prostatectomy on patients eighty years old or older with benign prostatic hyperplasia.

Combined prostatectomy and retropubic prostatectomy were performed on 20 patients eighty years old or older with benign prostatic hyperplasia. A comparative assessment of two surgical methods regading to operative blood loss, operating time and postoperative complications was presented. The procedure of combined prostatectomy revealed a smaller amount of operative blood loss, less operating time and lower incidence of complications when compared to retropublic prostatectomy. This modified method of suprapubic-retropubic prostatectomy appears to offer several advantages over other open methods of prostatectomy.

Aged↗
PubMed1986

[A case in which alpha-interferon showed remarkable effect against the brain, bone, and lung metastases arising from the renal cell carcinoma].

A 37-year-old man was sent to our department from the Radiology Department on July 19, 1984 with the diagnosis of right renal cell carcinoma with metastases to the right humerus and bilateral lungs. He was nephrectomized immediately and administered 10 X 10(6) units of alpha-interferon i. m. every other day for 3 months during the admission and 30 X 10(6) units once a week for more than 3 months after discharge. Although in addition to humeral and lung metastases, brain metastasis was found a week after the operation, interferon showed a remarkable effect and produced get a complete response in the humeral and brain metastases, and partial response in the lung metastasis. We think this is, perhaps, the first case of CR in brain metastasis so far. We conclude that interferon can be the first choice drug as an adjuvant chemotherapy against renal cell carcinoma.

Adult↗
PubMed1986

[Rhabdomyosarcoma of the prostate].

We report a case of rhabdomyosarcoma of the prostate. The patient was a 56-year-old man who complained of anal pain and dysuria. Tumor of the prostate was suspected after rectal examination. Multiple metastatic lesions were found in the lungs and liver. A needle biopsy of the prostate revealed rhabdomyosarcoma. He received chemotherapy, using Etoposide and responded slightly. Subsequently VAC-therapy was also performed. Although the patient improved temporarily, he died 4 months after admission.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1986

[Clinical study of NFLX (norfloxacin) in complicated urinary tract infections].

The new chemotherapeutic agent NFLX was orally administered 600 mg a day for 5 consecutive days in 44 cases having complicated urinary tract infection, and its clinical efficacy was evaluated. They consisted of 8 marked effective cases, 19 moderately effective cases and 17 ineffective cases, and its overall clinical efficacy was 61%. The bacteria disappeared in 10 cases, and decreased in 9 cases. Thirteen cases showed bacterial alternation, and 12 cases remained unchanged. By type of disease group, the efficacy was slightly inferior in the indwelling catheter group compared with that of the nonindwellt group.

Adult↗
PubMed1985

Effects of suramin on HTLV-III/LAV infection presenting as Kaposi's sarcoma or AIDS-related complex: clinical pharmacology and suppression of virus replication in vivo.

Suramin was given to ten outpatients with acquired immunodeficiency syndrome (AIDS) presenting as Kaposi's sarcoma (KS) or as an AIDS-related complex (ARC). Side-effects associated with the administration of 6.2 g of suramin over 5 weeks included fevers, rashes, urinary abnormalities, and transient rises in hepatic aminotransferases. Peak serum levels of over 100 micrograms/ml were attained. There was evidence of HTLV-III infectivity and replication in lymphocytes from four patients before therapy. The detectable virus level fell in each case by the time of the last dose, and in three cases it became undetectable at the end of therapy. In each case, viral replication was again detected in the weeks or months following the administration of suramin. Despite this in-vivo virustatic effect, no significant clinical or immunological improvement was observed using this short-term regimen. However, the results provide a rationale for investigating longer-term regimens.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Human T cell leukemia/lymphoma virus I infection and subsequent cloning of normal human B cells. Direct responsiveness of cloned cells to recombinant interleukin 2 by differentiation in the absence of enhanced proliferation.

A human T cell leukemia/lymphoma virus (HTLV)-I-infected B cell clone expressed Tac antigen on its cell surface and responded to recombinant interleukin 2 (IL-2) by increased production of IgM without any increase in proliferation. Anti-Tac antibody completely inhibited the IL-2-induced differentiation of this HTLV-I-infected B cell clone. This study demonstrates that HTLV-I can directly infect normal mature human B cells, and that the Tac antigen, which may be induced by infection with HTLV-I, is the functional receptor for IL-2-induced B cell differentiation. The availability of such cell lines and clones should provide useful tools to delineate precisely the differentiation step in the human B cell cycle.

Antigens, Surface↗
PubMed1985

3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.

The acquired immune deficiency syndrome (AIDS) is thought to result from infection of T cells by a pathogenic human retrovirus, human T-lymphotropic virus type III (HTLV-III) or lymphadenopathy-associated virus (LAV). In this report, we describe the antiviral effects of a thymidine analogue,3'-azido-3'-deoxythymidine (BW A509U), which, as a triphosphate, inhibits the reverse transcriptase of HTLV-III/LAV. This agent blocks the expression of the p24 gag protein of HTLV-III/LAV in H9 cells following exposure to virus. The drug also inhibits the cytopathic effect of HTLV-IIIB (a virus derived from a pool of American patients) and HTLV-III/RF-II (an isolate obtained from a Haitian patient that differs by about 20% in the amino acid sequence of the envelope gene from several isolates of HTLV-III/LAV, including HTLV-IIIB, analyzed so far). 3'-Azido-3'-deoxythymidine also completely blocks viral replication as assessed by reverse transcriptase production in normal human peripheral blood mononuclear cells exposed to HTLV-IIIB. Finally, at concentrations of 3'-azido-3'-deoxythymidine that block the in vitro infectivity and cytopathic effect of HTLV-IIIB, the in vitro immune functions of normal T cells remain basically intact.

Acquired Immunodeficiency Syndrome↗