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Biomedical subjects

H Mitani

Publications and source records attributed to H Mitani.

At least 163 records · Page 9Linked to original sources
PubMed1990

The repair of UV-irradiated plasmids transfected into cultured fish cells.

UV-irradiated plasmids (pNPV B and pBSCATSV) were transfected into RBCF-1 cells derived from a goldfish (Carassius auratus) and into a xeroderma pigmentosum (group A) cell line, XP20SSV. The frequency of stable neor transformation by pNPV B decreased in a dose-dependent manner. However, in spite of large differences in UV sensitivity detected in the colony formation assay, the dose-response curves of RBCF-1 cells and XP20SSV cells were almost the same. The photorecovery (PR) of transforming activity of UV-irradiated plasmids was confirmed in RBCF-1 cells but its extent was much smaller than that observed in the survival assay. The expression of the transfected cat (chloramphenicol acetyltransferase; CAT) gene after 24-h incubation in the dark was much more sensitive to UV irradiation when compared with the stable transformation assay. The extent of PR of cat gene expression in RBCF-1 cells was high and comparable with that of the survival assay. The CAT value of RBCF-1 cells transfected with UV-irradiated plasmids relative to that of unirradiated controls increased as incubation time in the dark after transfection became longer. This suggests that the UV lesions on the plasmids transfected in the RBCF-1 cells were repaired in the dark. The cat gene expression of UV-irradiated plasmids in XP20SSV was very low and independent of incubation time after transfection.

Animals↗
PubMed1990

[Tooth formation and eruption in skeletal Class II and Class III malocclusions].

The purpose of this study was to investigate the timing of the formation and eruption of the permanent teeth between Skeletal Class II and Class III malocclusions right before pubertal growth period. Samples consisted of twenty five Japanese girls of Class II and Class III malocclusions, respectively, with Class I girls as a control. Materials used in this study were panoramic X-rays, dental X-rays and intraoral photographs of each sample, all taken at age of 8 years. Lateral cephalometric X-rays, wrist bone X-rays and records of standing height were used to study the craniofacial characteristics and the stage of bone maturation. Results were as follows: 1. Lower teeth formation of Class III group tended to be advanced when compared with Class II group, especially at molars. 2. There was no significant difference between the two groups with regard to the timing of eruption except lower lateral incisors, but eruption of the upper second molar of Class III group tended to be delayed. Results suggested that the skeletal disharmony may be associated with different timing of formation and eruption of the permanent teeth.

Child↗
PubMed1990

[Relationship between late adolescent growth of mandible and maturity indicators--mandibular third molar, hand bones, body height--in Japanese boys].

The study was undertaken to investigate the individual growth timing of the mandible during late adolescent period. Materials used in this study were lateral cephalometric X-rays, hand bones X-rays, panorama X-rays and data of standing height of eighteen Skeletal Class I Japanese boys, all were longitudinally taken from 15 to 18 years old. Results were as follows: 1. Mandibular growth was continued to increase until 18 years old, but annual growth rate was decreased every year. 2. Growth increments of mandibular length was widely varied during 15 to 18 years old. 3. Ossification timing of MP3u and R-IJ stage were tended to delay in cases, represented large amount of growth. 4. Cases showing large amount of growth in the mandible tended to have large increments in body height. 5. There was less correlation between mandibular growth timing and maturation of lower third molars. In conclusion, the growth behavior of hand bones and body height could be used as indicators for predicting mandibular growth potentiality during late adolescent period.

Adolescent↗
PubMed1990

[Changes of frontal facial form occurred after correction of anterior reversed occlusion in children with TMJ dysfunction].

The purpose of this study was to investigate the facial pattern of early childhood patient with temporomandibular joint (TMJ) dysfunction (clicking) occurred after anterior cross-bite correction. Chin cap appliance was engaged in all cases, with or without minor intraoral mechano-therapy. Materials were consisted of postero-anterior cephalograms of 50 Japanese patients (6-9 years old), all showing anterior cross-bite with normal function of TMJ at pre-treatment stage. They were consisted of two groups, one was the group of 22 patients with TMJ dysfunction occurred after cross-bite correction and the other was the control group of 28 patients with no TMJ problem. Morphological measurements were done and compared between two groups at pre-treatment stage and after cross-bite correction stage. The results were as follows: 1. In many cases, the TMJ dysfunction was found at about 6 months later after anterior cross-bite correction. 2. It was cleared that the facial pattern of the TMJ dysfunction group showed asymmetry at pre-treatment stage and the same trend of asymmetry pattern continued after cross-bite correction. In conclusion, it was suggested that the TMJ dysfunction tends to occur in mandibular asymmetry patients after cross-bite correction stage because of the dynamic changes of occlusion and mandibular position.

Cephalometry↗
PubMed1990

[Multivariate analysis for the study of craniofacial structure. Selection of parameters on using the principal component analysis].

The present study was undertaken to discuss the effects of parameters on using the principal component analysis for the evaluation of the craniofacial structures. Materials consisted of lateral roentgenocephalograms of 100 adult Japanese females. They were divided into three groups by ANB angle. Linear and angular measurements were selected as parameters to apply the principal component analysis, and scattergrams made by the first and second principal component were compared. The results of the analysis were varied by the parameters. Especially there was a great difference between the real size and the value corrected by isometric method on scattergrams made by the first and second components. Results indicated the importance of using appropriate parameters for the research purpose.

Adult↗
PubMed1990

[A case of paratesticular rhabdomyosarcoma].

A case of paratesticular rhabdomyosarcoma in an 18-year-old male is reported. The patient was admitted for swelling of the right scrotum and right inguinal pain. Malignant tumor of the right testis was suspected and a high inguinal orchiectomy was performed. The pathological diagnosis was paratesticular rhabdomyosarcoma. Following retroperitoneal lymph node extirpation, chemotherapy, so-called CYVADIC therapy, consisting of cyclophosphamide, vincristine, adriamycin and dacarbazine was employed in 3 regions. The patient is in good health without recurrence 16 months after the surgery.

Adolescent↗
PubMed1989

Tumorigenic conversion of xeroderma pigmentosum lymphoblastoid cells without karyotypic alteration.

In order to examine the process of malignant transformation of human somatic cells, we studied the tumorigenic conversion of an Epstein-Barr-virus-immortalized lymphoblastoid cell line (LCL) derived from a patient with xeroderma pigmentosum (XP) complementation group A. Repeated irradiation of the XP cells, XP7NI, with UV-light and subsequent treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in the acquisition of tumorigenicity in athymic nude mice. The tumorigenicity of XP7NI cells was also induced by TPA treatment alone. The tumors formed in athymic mice were of B-cell lymphoma with characteristic histology, cell surface immunoglobulins and an antigen as detected by a B-cell-specific monoclonal antibody (MAb), CD20. The surface immunoglobulins and the HLA type of these tumor cells were identical with those of the parental cells. These malignantly transformed cells retained the same UV sensitivity, serum requirement, colony-forming ability in soft agar, and normal human karyotype as the parental cells. Unlike other tumorigenic lymphoblastoid cell lines, this XP lymphoblastoid cell line provides a unique case in that process(es) leading to tumorigenicity may be induced by UV and TPA without apparent karyotypic changes.

Animals↗
PubMed1989

Immunohistochemical demonstration of lysozyme and lactoferrin in salivary pleomorphic adenomas.

Immunohistochemical identification of lysozyme and lactoferrin was made in salivary pleomorphic adenomas (147 cases) and the staining patterns were evaluated with respect to the histological features and histogenesis. In normal salivary glands, the intercalated duct cells gave positive staining for lysozyme in major glands, and serous acinar cells, demilune cells, and interlobular duct cells were positive in minor glands. Lactoferrin staining was irregularly positive in serous cells and ductal epithelium. In pleomorphic adenomas, the reaction for lysozyme was positive in 14% (21/147) of the cases, and was confined to luminal cells of tubulo-ductal structures. Lactoferrin in pleomorphic adenomas was distributed in luminal tumor cells (51%; 75/147), in outer tumor cells (3%; 4/147), and in both luminal and outer tumor cells (5%; 7/147) in tubulo-ductal structures; it was also detected in plasmacytoid myoepithelial cells (5%, 8/147). However, modified myoepithelial cells and other types of neoplastic myoepithelial participants were negative for lactoferrin staining. The occurrence of both lysozyme and lactoferrin in salivary pleomorphic adenomas suggests their participation in the local defense mechanism in the tumor.

Adenoma↗
PubMed1989

5-Azacytidine-induced recovery of O6-alkylguanine--DNA alkyltransferase activity in mouse Ha821 cells.

Mouse Ha821 cells, Harvey murine sarcoma virus-transformed NIH3T3 cells, have extremely low O6-alkylguanine--DNA alkyltransferase (O6AGTase) activity and are hypersensitive to an anti-tumor chloroethylating agent 1-(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU). The treatment of Ha821 cells with a DNA demethylating agent, 5-azacytidine, resulted in an increase in the frequency of ACNU-resistant cell clones. All randomly isolated ACNU-resistant cell clones were found to have O6AGTase activity comparable to the level of the parental NIH3T3 cells. These results suggest that reversible loss of O6AGTase activity in Ha821 cells is caused at least in part by inactivation of the O6AGTase gene due to methylation of cytosine in the gene.

Animals↗
PubMed1989

More efficient excision repair of pyrimidine dimers in the specific DNA sequence than in the genome overall in goldfish cells.

Excision repair of pyrimidine dimers induced by 254 nm UV was examined in the genome overall and in a specific sequence containing a transfected gene for hygromycin B resistance, in RBCF-1 cells derived from a goldfish, by the use of UV endonuclease of Micrococcus luteus and alkaline agarose gel electrophoresis. More than 40% of dimers were removed from the specific sequence, while about 20% were removed from the genome overall, within 24 h after exposure to UV (2.5-7.5 J/m2).

Animals↗
PubMed1989

Eurythermic and stenothermic growth of cultured fish cells and their thermosensitivity.

RBCF-1 cells derived from the caudal fin of the goldfish (Carassius auratus) have been established at 37 degrees C, and could grow at a wide range of temperatures (20-37 degrees C). To examine the mechanism(s) of this eurythermic property, we isolated clones from RBCF-1 cells with different optimal growth temperatures. R37 clone grew continuously at 37 degrees C but could not grow at 27 degrees C. R27 clone grew continuously at 27 degrees C but could not grow at 37 degrees C. Thus R37 and R27 cells may be referred to as stenothermic. The R3727 clone, which was derived from R37 cells selected at 27 degrees C, and the cell hybrids of R37 and R27 cells, could proliferate for a long time and form colonies at 27-37 degrees C, like progenitor RBCF-1 cells, so they may be referred to as eurythermic. The shape of colonies of R3727 cells changed with incubation temperature; they resembled those of R37 cells at a higher temperature (37 degrees C), while at a lower temperature, (27 degrees C), they were like those of R27 cells. The lethal effects of heat treatment were compared among RBCF-1 clones. R27 cells were most sensitive, and R37 cells were most resistant. The heat treatment could induce the four major heat-shock proteins (hsp30, hsp42, hsp70 and hsp90). Marked induction of hsp70 and hsp30 was observed at 33 degrees C for R27, at 37 degrees C for R3727 and at 40 degrees C for R37. With R3727 cells, induction of hsps and thermosensitivity were highly dependent on the incubation temperature before heat treatment.

Animals↗
PubMed1989

[Partial nephrectomy for renal tumors].

We performed partial nephrectomy in 5 patients with renal tumors. In 1 patient with synchronous bilateral renal cell carcinoma, radical nephrectomy was conducted simultaneously for the contralateral kidney. The second patient had a transitional cell carcinoma of the renal pelvis of the contralateral side, for which the pelvis was resected simultaneously. In the remaining 3 patients the contralateral kidney was normal. In these patients the tumor was less than 3.0 cm in size and localized in either the upper pole or peripheral region, for which partial nephrectomy was performed. The weight of the resected kidney ranged 6 to 35 g with a mean of 18.2 g. The tumor size was 1.8 to 4.0 cm, the mean being 2.4 cm. Tumor grading was renal cell carcinoma grade 1 in 2 patients, grade 2 in 2 patients and oncocytoma in one patient. In all patients the tumor was encapsulated with a pseudocapsule with no involvement of the perinephric fat, venous or regional lymph nodes, and the tumor was diagnosed as pT2V0N0M0, stage I. In none of the patients intraoperative or postoperative complications developed with renal function of the operated kidney normal. The follow up interval after the operation was 5 years in 1 patients and 6 months to 1 year and 2 months in the remaining 4 patients. All patients have survived without metastasis or local recurrence in the operated kidney. We believe that partial nephrectomy is worth performing in selected patients with renal cell carcinoma not only in those having lesion in solitary kidney or bilateral kidneys but also in those whose function of the contralateral kidney is normal.

Adenoma↗
PubMed1989

[Abnormal findings on IVP study manifested by invasion of pancreatic carcinoma].

Pancreas carcinoma is sometimes notoriously difficult to diagnose. Although it is well known that pancreas carcinoma can invade contiguous organs, no attention to urinary tract involvement is paid. Although urinary tract is involved at a late stage, it may represent the first symptomatic evidence of the disease. In such cases, pancreas carcinoma can be presented initially as a urologic disease and patients' symptom may not be differentiated from urinary tract diseases. Computed tomographic (CT) images and medical records of 50 patients with histologically proved pancreas carcinoma were reviewed. There were 10 cases with urinary tract involvement. IVP study was performed in 8 of these cases. Of the 10 cases 8 were male and 2 were female. There was a high incidence of left urinary tract involvement (left:right:bilateral = 7:2:1). Nine cases were pancreas body and tail carcinoma and 1 case was pancreas head carcinoma. The stages were all in IV. All cases were with confirmed evidence of urinary tract involvement at autopsy within 4 months following CT studies. We compared those IVP, CT images and findings of autopsy. In these patients, 4 cases showed direct invasion of the kidney, 5 cases ureteral involvement and 1 case metastasis to the urinary bladder and ureters. IVP showed inferior displacement of the kidney and/or ureteral displacement on the affected side, as well as collecting system distortion, including hydronephrosis secondary to ureteral compression. CT scan showed an abnormal mass which is contiguous to the pancreas, showing compression of the kidney and/or retroperitoneal extension with ureteral involvement. Metastasis to the urinary bladder is also identified.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1989

[Evaluation on facial pattern of early childhood patients with T.M.J. dysfunction occurred after anterior crossbite correction].

The purpose of this study was to investigate the facial pattern of early childhood patient with temporomandibular joint (TMJ) dysfunction (clicking) occurred after anterior cross-bite correction. Chincap appliance was engaged in all cases, with or without minor intraoral mechano-therapy. Materials were consisted of lateral and postero-anterior cephalograms of 50 japanese patients (6-9 years old), all showing anterior cross-bite with normal function of TMJ at pre-treatment stage. They were consisted of two groups, one was the group of 22 patients with TMJ dysfunction occurred after cross-bite correction and the other was the control group of 28 patients with no TMJ problem. Morphological measurements were done and compared between two groups. The results were as follows: 1) There was no significant difference between the TMJ group and the control group from the lateral facial pattern. 2) The upper and middle facial skeleton were symmetry in both groups, but the maxillary alveolar legion (CMo, U 1) of TMJ group was significantly from the antero-posterior view. 3) On the lower face (mandible) of the TMJ group, it was pointed out that the position of Gonion was significantly asymmetry and L 1 point and Menton were disclosed to have severe lateral displacement. It was cleared that a high incidence of temporomandibular joint dysfunction was found in mandibular asymmetry cases. It was concluded that a careful case-management is required for mandibular asymmetry patients during chicap-orthodontic therapy.

Cephalometry↗
PubMed1989

[Radio-Geometric consideration of basal indentation using phantoms and specimens].

With the aid of colon-polyp phantoms, basal indentation (BI) of polypoid lesions on double-contrast examinations of the colon was studied. Our findings indicated that BI is a function of radio-geometric projections and its morphology (arcuate, angular and trapezoid) depends on size, shape and surface pattern of a polypoid lesion. These findings were also substantiated by specimen and clinical radiography.

Colonic Polyps↗
PubMed1989

[Longitudinal study on average craniofacial growth of skeletal C1. III girls in late adolescent period. Possibility of early orthognathic surgery].

The growth increments of Skeletal C1. III face during late adolescent period were investigated to estimate the possibility of earlier application of orthognathic surgery. Twenty C1. III Japanese girls, all requiring orthognathic surgery were investigated. Twenty C1. I girls were used as the control. The materials used in this study were lateral cephalometric X rays which were longitudinally taken at the age of 14 and 17 years respectively. Results were as follows: 1. The mean increments of maxilla and mandible showed no significant difference between C1. III and C1. I after the pubertal growth peak. 2. Ossification stage of the 3rd middle phalanx and the radium could be used as the maturity indicators for mandibular growth. Those were more reliable than the appearance timing of the menarche. Results are considered to be useful information for determining the timing of orthognathic surgery for Skeletal C1. III cases.

Adolescent↗
PubMed1989

[A partial nephrectomy in renal cell carcinoma].

Two patients with a renal cell carcinoma under went a partial nephrectomy. In both these cases the renal cell carcinoma had been detected incidentally and the lesion was a localized small tumor. Therefore, a partial nephrectomy was performed similarly, the tumor size was 1.8 x 1.7 cm in both cases, but the histologic grading was grade 1 in one case and grade 2 in the other though the stage was pT2VoNoMo, stage 1 in both. We thus consider it recommendable that a partial nephrectomy be performed not only in cases of a solitary or bilateral renal cell carcinoma but also in selected cases of a renal cell carcinoma with normal contralateral kidney.

Carcinoma, Renal Cell↗