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Biomedical subjects

H Minne

Publications and source records attributed to H Minne.

At least 37 records · Page 2Linked to original sources
PubMed1979

Radioimmunoassay for human parathyroid hormone for differentiation between patients with hypoparathyroidism, hyperparathyroidism and normals.

A RIA system for human PTH is presented using a goat antibody (Code name 017-spring-78) against C-terminal hPTH fragments, as well as a human PTH standard from hemodiafiltration of a hyperparathyroid patient. It proved to be useful for the differentiation not only between hyperparathyroid patients and normals, but even within the normal range and hypoparathyroid states.

Diagnosis, Differential↗
PubMed1978

Hypercalcitoninaemia in patients with pheochromocytoma.

As pheochromocytoma sometimes is accompanied by medullary thyroid carcinoma (in the sense of multiple endocrine adonomatosis type II = Sipple-Syndrome), serum calcitonin (CT) was measured by radioimmunoassay in 4 patients with pheochromocytoma. Before extirpation of the adreno-medullary tumor, serum CT was distinctly increased to 3 and 30 ng/ml in 2 of 4 patients, respectively. After removal of the tumor, serum CT was normal in the patients and pentagastrin stimulation produced no exaggerated CT response. In hydrochloric acid extracts from the two corresponding pheochromocytoma tissues, immunoreactive-Calcitonin (IR-CT) was detected, the concentrations amounting 1 and 4 ng/mg wet tissue. These findings suggest that hypercalcitonism in patients with pheochromocytoma cannot always be ascribed to the thyroid, i.e. increased calcitonin levels do not necessarily indicate a medullary carcinoma of this organ.

Adolescent↗
PubMed1977

[The determination of calcitonin for hospital use (author's transl)].

The significance of measuring calcitonin by biological and radioimmunological methods for diagnostic and therapeutic purposes is demonstrated in 18 cases of medullary thyroid carcinoma (MTC). The radioimmunoassay used permits the differentiation between normals (up to 0.5 ng/ml) and patients with MTC (3 to 120 ng/ml). As clinical symptomatology is often unspecific (tumor of the neck, perhaps in combination with diarrhea), the determination of calcitonin proved to be a useful preoperative diagnostic tool which can be improved by calcitonin stimulation tests (pentagastrin i.v.). Calcitonin values provide information on the completeness of surgical procedures or the recurrence of the tumor before clinical symptoms are manifest.

Adolescent↗
PubMed1975

[Parathormone determination with synthetic human parathormone 1-34 (PTH) (author's transl)].

Using synthetic human parathormone 1-34, a radioimmunological method for measuring this hormone fragment was developed which, as the amino-terminal PTH assay, permits the measurement of the concentration gradient in the neighborhood of OTH-secreting tumors. The use of preoperative location diagnosis in primary hyperparathyroidism is demonstrated with some typical cases, especially in revision operations.

Adenoma↗
PubMed1975

[Observations in vitamin D and dihydrotachysterol poisoning].

In three women intoxication with vitamin D or dihydrotachysterol occurred. Two patients died from complications despite successful lowering of the serum calcium, the third died after a pulmonary embolus during hypercalcaemia 5 months after cessation of vitamin D. Correct observation of the narrow therapeutic range of vitamin D preparations appears most important in the treatment of hypoparathyroidism and other indications. Particular attention should be given to the prophylaxis of over dosage. Apart from regular serum calcium estimations instruction of the patient and relatives as to the dangers and symptoms of intoxication is recommended. The issuing of a therapy identity card would meet these requirements.

Aged↗
PubMed1975

Pancreatic enzyme secretion in the conscious rat. Method and application.

1. A method for collecting duodenal juice and gastric content separately, in conscious rats, is described. Metal cannulas were implanted into the stomach fundus. For the main experiment a double lumen tube was inserted through the cannula via the pylorus into the duodenum. 2. The following secretion patterns were observed: a) In the resting state there was a constant flow rate of duodenal volume, bicarbonate, trypsin and amylase. b) Cholinergic stimuli were capable of increasing enzyme secretion as much as fourfold for a period of 30 to 40 min when administered as a single subcutaneous injection. This effect was annulled by atropine. c) Secretin and cholecystokinin-pancreozymin given together in a single injection s.c. or i.v., elicited a similarly strong response. d) Identical ranges of the secretion maxima were found with a tendency to decrease after the first hour, when the hormones were infused either s.c. or i.v. e) Doses from 0.5 to 25 U/100 g b.w. /hr showed identical responses. Doses below 0.2 U/100 g/hr were without effect. 3. Narcosis (pentobarbital) inhibited markedly the resting and stimulated enzyme secretion. 4. The method is suitable for examination of physiological and pharmacological effects on resting and stimulated enzyme secretion of the rat pancreas.

Amylases↗
PubMed1975

The effect of chronic lithium application on the mineral content of rat bone and soft tissues.

Rats were treated with lithium chloride over 6 weeks. During the experiment, determinations of serum calcium, magnesium and phosphorus revealed no changes due to the therapy. Postmortem examinations of liver, skeletal muscle, and femur minerals as well as bone histology showed these organs to be unaffected by lithium application. Only body weight gain was in part increased by lithium.

Animals↗
PubMed1975

The hypercalcaemic syndrome in rats bearing the Walker carcinosarcoma 256.

The strain of Walker carcinosarcoma 256 described induces hypercalcaemia, hyperphosphataemia and hyperuraemia in tumour bearing rats. Changes in calcium and phosphorus excretion are observed as well as accompanying calcification of soft tissue organs and loss of bone calcium. These changes in calcium metabolism disappear after removal of the tumour, so that long-range action of the tumour can be stated. The results are discussed in comparison with three other animal models of tumour dependent hypercalcaemia.

Animals↗