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Biomedical subjects

H Miki

Publications and source records attributed to H Miki.

At least 307 records · Page 17Linked to original sources
PubMed1983

Histochemical studies on enzyme-digested protein plugs of patients with chronic pancreatitis: a preliminary report.

Sulfated acidic mucopolysaccharides have been found to be significant components of "protein plugs" in patients with chronic pancreatitis. The precise identification of the mucopolysaccharides and their distribution within the protein plugs may clarify the pathogenesis of the plugs. Pure pancreatic juice from five patients with chronic pancreatitis was obtained by endoscopic retrograde catheterization of the papilla of Vater. Enzymes for digestion of the plugs included hyaluronidase of the bovine testes and streptomyces hyalurolyticus, chondroitinase ABC and AC, and sialidase (neuraminidase). Our study indicated that: I) Sialic acid is distributed throughout the plugs and may be a major component, followed by a lesser amount of chondroitin sulfate B. 2) Chondroitin sulfate A, C, D and E and chondroitin may be minor components. 3) Hyaluronic acid is negligible in the plugs.

Chronic Disease↗
PubMed1983

[26-week intravenous toxicity test of cefpiramide in cynomolgus monkeys].

A chronic toxicity of cefpiramide (CPM) was studied in Cynomolgus monkeys. Groups of 4 males and 4 females were given daily doses of 100, 300 mg/kg or 600 mg/kg by intravenous administration for 26 weeks. Another group of 4 males and 4 females was given physiological saline and served as the control. In CPM groups, diarrhea or soft feces was observed after the commencement of administration at every doses. The frequency of these signs decreased as the study progressed and animals at lower doses returned to normal earlier. There were no treatment-related changes in body weights, fecal occult blood and electrocardiograms. A female in the 600 mg/kg group died after 13 weeks of treatment. The cause of death was assumed to be an acute myocardial necrosis resulting from thromboarteritis. It was not considered to be treatment-related. Ophthalmological examination revealed no abnormalities attributable to the treatment. Erythrocyte counts, hemoglobin and hematocrit values decreased in 3 animals receiving 600 mg/kg of the drug. The changes, however, were transient at least in 2 of these animals. There were no effects on plasma biochemical and urinalysis parameters. Small yellow spotes were noted in the renal cortex of 2 monkeys in the 600 mg/kg group at necropsy. Liver and kidneys were slightly heavier in animals receiving 600 mg/kg. Histopathological examinations revealed focal nonsuppurative interstitial nephritis in 1 animal given 300 mg/kg and 5 animals given 600 mg/kg. No treatment-related changes were observed in other organs and tissues. From these results, the maximum non-effective dose level of CPM was considered to be 100 mg/kg.

Animals↗
PubMed1983

[6-week intravenous toxicity test of cefpiramide in rhesus monkeys].

A subchronic toxicity of cefpiramide (CPM) was studied in rhesus monkeys. The drug was administered intravenously to 4 groups of 2 males and 2 females each at a daily dose of 0, 100, 300 mg/kg and 1,000 mg/kg, respectively, for 6 weeks. Vomiting and diarrhea were observed at every dose of CPM, more frequently at the beginning of the treatment period. In addition, salivation was observed at 300 mg/kg or more and a transient subdued mood and inappetence at 1,000 mg/kg. A slight decrease of body weight was noted in a female given 1,000 mg/kg. There were no treatment-related changes in fecal occult blood test and electrocardiograms. Ophthalmological examinations showed no abnormalities being attributable to the treatment. A transient or slight anemia was observed in 2 monkeys receiving 1,000 mg/kg. Examination of bone marrow revealed no abnormalities. There were no effects on plasma biochemical parameters. In urinalysis, a female in the 1,000 mg/kg group showed a glycosuria. Kidneys of 3 animals given 1,000 mg/kg were pale at necropsy. Liver and kidneys were heavier in some animals at 300 mg/kg or more and at 1,000 mg/kg, respectively. Histological examination revealed a multifocal degeneration and regeneration of the proximal renal tubular epithelium in all animals receiving 1,000 mg/kg. No treatment-related changes were encountered in other organs and tissues. From these results, the maximum non-effective dose level of CPM was considered to be 300 mg/kg.

Animals↗
PubMed1982

Role of endoscopic elimination of protein plugs in the treatment of chronic pancreatitis.

The purpose of this report is to evaluate the role of endoscopic elimination of protein plugs in the treatment of chronic pancreatitis (CP) and suspected CP. Endoscopic aspiration of pure pancreatic juice (PPJ) was performed on 69 patients with CP or suspected CP. PPJ was collected from within the main pancreatic duct by endoscopic retrograde catheterization of the papilla after a rapid intravenous injection of secretin and CCK-PZ. Following results were obtained. (1) Various numbers of protein plugs were obtained along with PPJ in 26 of the 69 patients. (2) Endoscopic elimination of protein plugs provided 17 of the 26 patients with dramatic relief from abdominal pain and back pain, indicting that the procedure was often useful, at least, for relieving pain in patients with protein plugs in the pancreatic duct system. (3) Follow-up studies suggested that the procedure could be an effective therapeutic tool in selected cases of CP or suspected CP in which no prominent stenotic lesions were noted in the major pancreatic duct system and abstinence from alcohol beverage was strictly observed. (4) In 43 patients with no protein plugs in the pancreatic juice, in contrast, transient or partial relief from abdominal pain was provided in only one patient, respectively.

Abdomen↗
PubMed1982

Effect of clinofibrate, a new hypolipidemic agent, on biliary and serum lipids in patients with hyperlipidemia.

Clinofibrate was given to 15 patients with hyperlipidemia, for 6-8 weeks at the daily dose of 600 mg, and its effect on 3 biliary lipid components (cholesterol, bile acids and phospholipids) and on the lithogenic index was investigated. After clinofibrate treatment, 6 of the patients were given 1.5 g/day clofibrate for 6-8 weeks to compare the effect of clofibrate with that of clinofibrate. The molar percentages of biliary cholesterol and phospholipids to the total mol number of the 3 biliary lipid components decreased, and that of bile acids increased during clinofibrate administration. In this way, the molar ratio of bile acids to cholesterol increased during the treatment. Neither the lithogenic index calculated by the formula of Admirand and Small nor that of Hegardt, Dam and Holzbach was altered significantly by the treatment. There was no apparent relationship between the effect of the drug on the lithogenic index and any of the factors initial lithogenic index, rate of decrease of serum lipids, or type of hyperlipidemia. Although clofibrate had no significant effect on the maximum solubility of cholesterol in the bile, the molar percentage of biliary cholesterol was elevated and the lithogenic index increased as compared with the control and clinofibrate period. No significant influence on bile acid composition in the bile was observed, with either clinofibrate or clofibrate.

Adult↗
PubMed1982

Characteristics of gait ataxia in spinocerebellar degeneration assessed by means of simple quantitative method.

Cerebellar ataxic gait was compared with normals by a simple quantitative method. Strain gauges attached to the heel, the first and the fifth metatarsal areas of a shoe, were used and the gait was telemetrically recorded. A natural and one per second click sound-synchronized gait was analyzed. A contact sequence of the three areas of the sole, the stride time, the stance-phase time and the foot contact pattern were compared between patients and normals. All of these items disclosed different results in the two groups.

Adult↗
PubMed1981

The crystal and molecular structure of 2'-deoxy-2'-fluoroinosine monohydrate.

The structure of the hydrate of 2'-deoxy-2'-fluoroinosine has been determined by single-crystal x-ray diffraction. The nucleoside crystallizes in space group P2(1)2(1)2(1) with unit cell dimensions, a = 33.291, b = 10. 871, c = 6.897A. There are two nucleosides and two water molecules in the asymmetric unit. The structure was solved by direct methods and refined to a residual R = 0.095. The two independent nucleosides in the asymmetric unit show different conformations about the glycosidic bond, while other structural details are similar. The base orientation to the sugar is syn in molecule A, whereas anti in molecule B. The exocyclic C(4')-C(5') bond conformation defined with respect to C(3')-C(4')-C(5')-O(5') is gauche+ in both molecules A and B. The sugar ring pucker defined by the pseudorotation phase angle P is a twisted conformation in both, C(3')-endo-C(4')-exo with P = 29 degrees in molecule A and C(4')-exo-C(3')-endo with P = 41 degrees in molecule B. It is shown by comparison with x-ray results of other 2'-fluoronucleosides and unmodified nucleosides including inosines that, in addition to a strong preference of the C(3')-endo type pucker, twisted conformations involving C(4')-exo puckering may be one of characteristic features of 2'-fluoronucleosides.

Chemical Phenomena↗
PubMed1981

Determination of barbiturates in mouse tissues by high-performance liquid chromatography.

Procedures for determining barbiturates in mouse tissues were investigated. High-performance liquid chromatography (HPLC) with mixtures of water and methanol as the mobile phase and muBondapak C18 as the stationary phase is superior to gas and thin-layer chromatography with respect to ease of sample preparation, accuracy, sensitivity and time required for analysis. The first step in the analysis, the extraction of barbiturates from tissues, was also investigated and good recoveries were achieved. The time courses of barbiturate concentrations in mouse brain, kidneys and liver after oral administration of barbiturate-beta-cyclodextrin complex to mice were determined by HPLC using UV detection at 210 nm.

Animals↗