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Biomedical subjects

H Mickley

Publications and source records attributed to H Mickley.

67 records · Page 4Linked to original sources
PubMed1985

The significance of platelet-associated immunoglobulin G in non-thrombocytopenic patients with systemic lupus erythematosus.

The possible pathogenetic significance of platelet-associated immunoglobulin G in systemic lupus erythematosus (SLE) has been studied, using a semiquantitative immunofluorescence technique. The study included 22 patients suffering from SLE during the period 1973-81. Thirteen patients had various clinical and/or laboratory signs of disease activity at the time of investigation, but none showed thrombocyte values below the normal range. A positive reaction for platelet-associated immunoglobulin in serum (indirect PlIgG) was demonstrated in 73 percent of the cases and in two patients on their own platelets also (direct PlIgG). The study included investigation of ANA (80% positive) and anti-DNA (positive in three patients only). A significant negative correlation was found between indirect PlIgG and platelet count and between ANA (/IgG or IgM) and platelet count. Indirect PlIgG correlated with known expressions of disease activity, as high ESR and low Hb, and in patients with direct PlIgG, several markers of disease activity were found to be present. It is concluded that PlIgG may be of pathogenetic importance and can be used as a marker of disease activity.

Adolescent↗
PubMed1984

Malaria-induced immune thrombocytopenia.

On return from Liberia, a previously healthy 36-year-old man showed signs of malaria accompanied by severe haemolysis and slight thrombocytopenia. We found evidence of a platelet-associated IgG being responsible for the thrombocytopenia, inasmuch as the direct platelet suspension immunofluorescence test was strongly positive, the indirect immunofluorescence test and tests for drug-dependent antibodies at the same time being negative. We suggest that autoimmunity may be a contributing mechanism for platelet destruction in acute malaria.

Adult↗
PubMed1984

Immune haemolytic anaemia associated with ampicillin dependent warm antibodies and high titre cold agglutinins in a patient with Mycoplasma pneumonia.

A case of severe immune haemolytic anaemia in a 54-year-old man suffering from Mycoplasma pneumonia is presented. A strongly positive direct Coombs test with erythrocyte bound IgG, C3d and C4 was demonstrated during the haemolytic process. Further, serologic investigations revealed ampicillin-dependent 'warm' antibodies and a cold agglutinin titre greater than 2000. No firm conclusion can be drawn as to which antibody caused the haemolysis. Treatment including withdrawal of antibiotics, blood transfusions (washed red cells, concentrated erythrocyte suspension) and corticosteroids was successful. It is suggested that tests should be carried out for erythrocyte bound drug-induced antibodies in cases of haemolysis with a history of drug exposure.

Agglutinins↗
PubMed1983

Natural killer cell activity during premedication, anaesthesia and surgery.

Natural killer (NK) cell activity of peripheral blood mononuclear cells was measured against K-562 target cells in a 51Cr release assay in eight patients undergoing total hip replacement surgery. Eight consecutive blood samples were taken from each patient. A significant increase of NK cell activity was observed after premedication with diazepam per os. The activity increased further during a combined anaesthesia (thiopentone + N2O + O2 + buprenorphene + pancuronium) and remained increased during surgery. Postoperatively, NK cell activity fell and remained depressed for a period of at least 5 days. The findings of this study indicate that premedication, anaesthesia and surgery cause a rapid and transient increase in NK cell activity, followed by a decline in activity postoperatively. The transient increase in activity may be explained by mobilization of natural killer cells from extravasal space, spleen or lymph nodes into the circulation. The clinical significance of the alterations in NK cell activity is unknown and needs further investigation.

Aged↗