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Biomedical subjects

H Meffert

Publications and source records attributed to H Meffert.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Percutaneous absorption of hematoporphyrin derivative--fluorescence- and electron microscopic studies].

Using surviving human skin, percutaneous absorption following topical application of hematoporphyrin derivative was investigated. Hematoporphyrin derivative gave strong red fluorescence, which was dependent from it's actual concentration, from the used kind of vehicle, from preparation time, and from time between preparation and application of hematoporphyrin derivative solution. Ultrastructural investigation verified cytoplasmatic membranes and mitochondria as the principal targets also after topical application followed by irradiation with visible light.

Hematoporphyrin Derivative↗
PubMed1990

[Therapy of acne with visible light. Decreased irradiation time by using a blue-light high-energy lamp].

Lipophilic propionibacterium acnes stores large amounts of porphyrins. This peculiarity can be used for photodynamic destruction of propionibacteria. After repeated irradiations with a blue light-type high pressure lamp (Narva NC 1000-64) both acne and seborrhoea was improved markedly (10 irradiations, each for 10 minutes, cumulative light dose 325 J/cm2). Also porphyrin content inside acne follicles dropped. Serial irradiations with short range visible light (400 to 420 nm) are recommended for acne treatment during the light-poor season of the year.

Acne Vulgaris↗
PubMed1990

[Systemic effects of ultraviolet, visible and infrared radiation in serial whole body irradiation. II. Effect on the immune cells of the skin].

Before and after skin treatment by ultraviolet radiation, infrared radiation or visible light the ability of polymorphonuclear leukocytes (PMN) to form oxygen intermediates was investigated in 31 healthy volunteers. Superoxide production was evaluated photometrically as the reduction of Iodonitrotetrazoliumchloride (INT). After whole body treatment by 4 UV exposures all persons exhibited significantly enhanced rates of INT-reduction for 26-62 days. The in vivo half-time of PMN is 5-7 hours. Therefore a direct effect of irradiation over that long period is rather unlikely, suggesting that mediators should play a crucial role in signal transduction from the skin to the peripheral blood cells. Most likely candidates for connective links are interferon-gamma (IFN-gamma), granulocyte-monocyte colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF).

Free Radicals↗
PubMed1990

[Prolongation of transplantation survival time by a PUVA treatment. Experimental and clinical results].

The treatment of transplants by a combination of 8-methoxypsoralen and longwave ultraviolet radiation (PUVA) leads to prolongation of the transplants in allogeneic receivers without immunosuppression. The rejection of skin grafts in the mice could be delayed significantly. The rejection of kidney and heart allografts could be prevented completely. A significant diminution of the acute rejection crises and a raising of the one-year survival rate showed a first clinical study by means of PUVA treatment in human kidney grafts.

Animals↗
PubMed1990

[The cytotoxic effect of anti-Ro- (SS-A) antibodies and UVA light on human endothelial cells in vitro].

There is in photosensitive lupus erythematosus a strong association of high titers of anti-Ro(SS-A) antibodies in the serum of the patient with the development of special skin reactions depending on UV-light. The aim of our experiments was to detect a cytotoxic effect of anti-Ro(SS-A) antibodies and UVA-light on human endothelial cells in vitro (standard trypan blue exclusion test, SEM). After UVA-doses of 1 to 100 J/cm2, which were tested, and influence of serum containing anti-Ro(SS-A) antibodies (62 E, ELISA) membrane destructions of endothelial cells depending on the UVA-dose can be seen, irrespective of the fact whether the antibodies are present throughout the irradiation or added afterwards. This cytotoxic reaction depends on complement. A UVA-dose of 100 J/cm2 causes lethal damage of 40% of the cells. These results were confirmed by scanning electron microscopy.

Antibodies, Antinuclear↗
PubMed1990

[Promotion of hair growth with thiocyanate in guinea pigs].

The advancement of growth of albino guinea pig hair has been induced by sodium thiocyanate through oral application (32 mg/kg) and more distinctly through balneological use (0.3, 5 resp. 10 g/l). The density of the hair has been increased at 6-38%, the longitudinal growth advanced at 2-14% and the phases of follicle cycle have been changed on behalf of anagenic hair at 11-39%.

Administration, Oral↗
PubMed1990

[Patients born in autumn more frequently develop atopic eczema].

Data of birth from 4040 atopic dermatitis patients born in the Berlin area were analyzed in view of their distribution over the 12 months of the year. For comparison the data of birth of all people being born during two years were treated the same way. There was a marked increase of atopic dermatitis with the persons born during the months August to October. On the other hand, the prevalence of atopic dermatitis was diminished with that persons who were born from February till July (with exception of May). Obviously, during the first months of life seasonally differing external factors influence directly o indirectly the decision, whether atopic dermatitis later on will manifest or not. These results should been taken into consideration for family planning in high-risk families.

Adolescent↗
PubMed1989

[Serial infrared and ultraviolet whole body irradiation and placebo and ultraviolet irradiation of autologous venous blood in peripheral arterial occlusive disease. 1. Treadmill ergometry, metabolic, rheologic and hemodynamic parameters].

In 21 patients suffering from obstructive peripheral arterial disease stage II according to Fontaine, therapeutic efficacy of serial whole body irradiations (infrared or ultraviolet radiation) and pretended or real ultraviolet light blood irradiations was evaluated. Before, during and after treatment the following parameters were monitored: walking distance, oxygen partial pressure (quasi-arterial/venous), flow properties of blood (appearant blood viscosity, hemodynamics (peak flow, ultrasonics). There were no significant changes following both modalities of whole body irradiations either by sunshine-like ultraviolet light nor by infrared radiation, nor by pretended blood irradiation. In the same patients mean walking distance was prolonged threefold after ultraviolet irradiation of the patient's own venous blood and subsequent retransfusion. Simultaneously, oxygen utilisation was improved (enlarged arterial/venous difference), lactate concentration was decreased and apparent blood viscosity was diminished, whereas blood flow remained unchanged or only slightly improved. In this way the circulus vitiosus of obstructive peripheral arterial disease can be overcome. As a consequence of blood irradiation walking distance enlarges, providing better chances for physical training, which helps to extend walking distance furthermore.

Adult↗
PubMed1989

[Effect of a single mild infrared A hyperthermia on body temperature, heart rate, blood pressure and blood viscosity in healthy probands and patients with stage I and II arterial hypertension].

Report on mild and single whole body-irradiations with near infrared (IRA). The central body temperature went up to 38.5 degrees C in 9 healthy subjects and 9 patients known as having essential arterial hypertension of the stages I or II. In case of hypertensive patients a single exposure decreased significantly both arterial and venous blood pressure as well as the resulting mean arterial blood pressure. This beneficial effect lasted for at least 24 hours. Besides that, improvement of peripheral hemodynamics and plasma viscosity also was proven. Obviously, improved peripheral hemodynamics is linked to that blood pressure lowering effect which happens due to dilatation of peripheral blood vessels.

Adult↗
PubMed1989

[Hemodynamics of patients with essential hypertension in mild infrared hyperthermia].

Hyperthermia therapy in hypertensive patients is been discussed as an effective treatment. The aim of this study was to control the behaviour of circulation in conditions of hyperthermia. A group of 6 males, 32-53 years old, suffering from essential hypertension stage I-II underwent a single whole body-irradiation with near infrared (IRA), lasting over one hour. The body temperature rises up to maximal 38.5 degrees C. Before, during and after treatment the blood pressure (BP), the heart rate (HR), the stroke volume (SV), the cardiac output (CO) and the ejection fraction - measured by radionuclide ventriculography - where estimated and the peripheral arterial mean pressure (Pm), the ejection resistance (AW) and the Total peripheral resistance (TpW) were calculated. During irradiations there was a significant decrease of BP, AW and TpW. HR, SV, CO and EF increased significantly. Due to IRA irradiations there was a change in microcirculation with the consequence of lowering the blood pressure. The concomitant stimulation of the heart was well compensated as shown by CO, SV and EF.

Adult↗
PubMed1989

[Photodynamic therapy of psoriasis palmaris et plantaris using a topically applied hematoporphyrin derivative and visible light].

Two different hematoporphyrin derivative (HpD) containing ointments were checked for their photodynamic efficacy in 17 patients suffering from palmoplantar psoriasis. Following topical application and subsequent irradiation with visible light, in all the patients psoriatic plaques resolved either to some substantial degree or totally. That method of treatment so far is recommendable for treating psoriasis of markedly hornifyed skin regions. However, further efforts are needed to perfect therapeutical circumstances like preparation time, spectral distribution of light and galenics of HpD-containing ointment.

Adult↗
PubMed1989

[Systemic effects of ultraviolet, visible and infrared radiation in serial whole body irradiation. I. Oxygen utilization, flow properties of blood, hemodynamics, blood components and phagocytosis].

31 healthy volunteers at the age of 19 to 29 years underwent whole-body irradiations by either ultraviolet radiation (NARVA UVS 65-2; continuous sunshine-like emission, predominantly UVA plus 8% UVB; cumulative doses after 4 and 20 irradiations 8.8 J/cm2 and 51.0 J/cm2, respectively), or visible light (emission of a 3,500 Watt lamp HGMI 3500 DL, Tungsram CSSR, filtered through 6 mm window glass; cumulative dose 267.0 J/cm2 after 4 irradiations) or infrared radiation (250 Watt infrared lamps NARVA "Biotherm", emitting more than 70% infrared radiation; cumulative dose 159.0 J/cm2 after 4 irradiations). Before, during and after the irradiation series the following investigations were made: Arterial and venous oxygen pressure, oxygen utilization index; flow properties of peripheral blood (plasma viscosity, erythrocyte aggregation kinetics, apparent blood viscosity); hemodynamics (veineal plethysmography, 133Xenon clearance, functional diameter of small blood vessels, peripheral blood pressure), phagocytotic capacity of polymorphonuclear white blood cells; hematological parameters (blood sedimentation rate, polymorphonuclear and eosinophilic blood cells, red blood cells, hemoglobin, hematocrit), serum proteins (IgG, IgA, IgM, complement C3, alpha-1-glycoprotein, alpha-1-antitrypsin, haptoglobin, transferrin); calcium and phosphate in serum. As far as irradiance, dose and treatment frequencies are concerned, the experimental conditions were very similar to those in phototherapeutical practice. Under these circumstances there were no hints for unwanted early effects after application of all the three kinds of optical radiation used in that study. Considerable systemic responses exclusively were found by use of ultraviolet radiation. Under these experimental conditions according to dermatological phototherapy it has been proven the following biopositive systemic responses are due to happen: increase of serum calcium and phosphate, improvement of blood oxygen utilization, improvement of blood flow properties, and enhanced phagocytic capacity of polymorphonuclear white blood cells. Regarding the parameters taken in that study, no early unwanted side effects are to expect after therapeutical application of rather large doses of visible light or infrared radiation.

Adult↗
PubMed1989

[Willibald Gebhardt (1861-1921). His life and his achievements in photomedicine].

As far as history of sports is concerned, Dr. Willibald Gebhardt is well known as an energetic and excentric chemicist who founded and lead the first ever German Olympic Team to the games at Athens, in despite of the declared will of both Prussian government and sports functionaires. The non-physician Gebhardt achieved distinct progress in photomedicine und hygiene which was holded back or doubted by the experts during his lifetime. Gebhardt's book "Die Heilkraft des Lichtes" printed in 1898 represents a milestone of photomedicine as well as a goldmine in view of future photomedical developments.

Germany↗
PubMed1989

Influence of a transfusion of donor leukocytes treated with 8-methoxypsoralen and long-wave ultraviolet light (PUVA) on skin allograft survival in mice.

The influence of pretransplant donor spleen cell infusions on murine skin graft survival was studied. In dependence on the time interval between transplantation and transfusion an accelerated or delayed rejection of the grafts was observed. If the donor spleen cells were treated with the photosensitizer 8-methoxypsoralen and UVA light (PUVA) a graft prolongation was achieved at all time intervals. Furthermore, the survival of antigenically unrelated grafts was also prolonged. An additional immunosuppressive treatment or the recipients with antilymphocyte serum, but not cyclophosphamide, led to a further prolongation of graft survival. The survival of PUVA treated skin grafts was not longer in recipients preinfused with PUVA treated donor cells compared with untreated hosts. The results presented in this work may have implications in clinical organ transplantation to prevent sensitizing reactions by sparing protective mechanisms for the graft.

Animals↗