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Biomedical subjects

H Matthies

Publications and source records attributed to H Matthies.

At least 181 records · Page 10Linked to original sources
PubMed1976

Changes in labeling of soluble and solubilized hippocampus proteins after a learning experiment in rats.

At various intervals after acquisition of a brightness discrimination in rats labeled leucine was intraventricularly applied. Hippocampus tissue was fractionated in soluble and solubilized insoluble protein fractions. Protein content and labeling of several electrophoretically resolved bands showed a biphasic time course: a first increase was observed 20 minutes after training including preferably soluble proteins, whereas a second increase (about eight hours after training) was mainly related to solubilized insoluble proteins.

Animals↗
PubMed1975

Free and membrane-bound ribosomes and polysomes in hippocampal neurons during a learning experiment.

The ribosomes of the CA1 and CA3 pyramidal cells of hipocampus were investigated by morphometric methods after the acquisition of a shock-motivated brightness discrimination in rats. A significant increase in the total number of ribosomes was observed in CA1 cells of trained animals and in CA3 cells of both active controls and trained rats. A significant increase in membrane-bound ribosomes was obtained in CA1 and CA3 cells after training only. The results confirm the suggestion of an increased protein synthesis in hippocampal neurons during and after the acquisition of a brightness discrimination, as we have concluded from out previous investigations on the incorporation of labeled amino acids under identical experimental conditions. The results lead to the assumption that the protein synthesis in some neuronal cells may probably differ not only quantitatively, but also qualitatively in trained and untrained animals.

Animals↗
PubMed1975

[Effects of brain extract and hydrolysate on nerve tissue in vitro].

Explants of PNS and CNS of chick embryos or fetal rats were cultivated and brainhydrolysat cerebrolysin or a synthetic aminoacidsolution or a brain extrakt was applied to the cultures. Cerebrolysin exercised in vitro an effect, which is related to charge only trifling. The cerebrolysin effect is essential related to the concentration/medium. High concentration of cerebrolysin decreases the growth of nerve fibers. Migration of non-neuronal cells increases. A stimulation of nerve fibers growth resembling to NGF was not seen. The density of cells or number of cells/volume unit, rate of mitosis and phases of mitotic non-neuronal cells is changed by cerebrolysin. The effect of cerebrolysin in vitro is compared to the tested aminoacidsolution and brainextract resembling. NGF may be important selectiv for stimulation of the activity of sympathetic and sensoric ganglia. Brainextract and brainhydrolysat and aminoacidsolution influenced in vitro PNS- and CNS-tissue cultures.

Animals↗
PubMed1975

[Micromethod for the quantitative determination of leucine from biological experimental material using a dansylation technic].

A method is described allowing quantitative determination of leucine from biological material up to 1 - 10(-11) M by means of dansylation of amino acids and their thin-layer chromatographic separation on polyamide microfoils. By selecting [14C]-U-L-leucine as an 'internal' standard, which is added to the tissue during the homogenization procedure, it is possible to correct for data scattering related to variations of the conversion rate or the yield of the dansylation reaction, and to losses in the different steps of thin-layer chromatography that are difficult to standardize.

Animals↗
PubMed1975

Changes in labeling of soluble and solubilized rat brain proteins using (3H)-leucine as precursor during a learning experiment.

This paper deals with an electrophoretic study on soluble and solubilized rat brain proteins during a brightness discrimination. After intraventricular injection of L-[3H]-leucine the most pronounced increase in relative specific activity was found for several soluble acidic protein-band complexes as well as for solubilized slow-migrating non-myelin proteins obtained from hippocampus of trained animals over the data obtained for active and passive controls.

Animals↗
PubMed1975

[Reliable micromethod for determination of the protein content in tissue homogenates].

A micromodification for protein determination in tissue material using Amido black 10 B is described. Compared with the method of LOWRY et al. it requires a comparable time expenditure, but has three principal advantages: 1) it is 5-10fold more sensitive; 2) the calibration curve is linear over a virtually unlimited range; 3) it is feasible in the presence of a number of substances frequently used in protein analyses and making difficult or impossible measurement according to LOWRY et al.

Animals↗
PubMed1975

Changes in labeling of soluble and insoluble rat brain proteins using [3H]-tyrosine as precursor during a learning experiment.

The incorporation of intraventricularly injected L-[3H]-tyrosine into proteins from three rat brain regions was studied during the acquisition of a shock-motivated brightness discrimination. Separating the proteins by means of polyacrylamide gel electrophoresis revealed an increased labeling confined to only a few of the resolved slow-moving bands of soluble and, especially insoluble hippocampus proteins. The labeling of visual cortex proteins showed only minute changes, while the auditory cortex proteins exhibited no differences between trained and nontrained animals.

Animals↗