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Biomedical subjects

H Masuda

Publications and source records attributed to H Masuda.

At least 613 records · Page 34Linked to original sources
PubMed1980

Purification and Some Properties of Cell Wall-bound Invertases from Sugar Beet Seedlings and Aged Slices of Mature Roots.

Cell wall-bound invertases (EC 3.2.1.26) from both sugar beet seedlings and aged slices of mature roots were purified to homogeneity separately with CM-cellulose chromatography and Bio-Gel P-150 gel filtrations. The enzymes behaved similarly throughout the purification procedures. The purified enzymes are identical as characterized by specific activity, gel electrophoretic mobility, K(m) for sucrose and raffinose (1.33 and 4.0 millimolar, respectively), mobility on Bio-Gel P-150 (molecular weight 28,000), optimum pH (4.6 to 5.0), optimum temperature, and dependence on NaCl concentration for insolubilization by DNA. The results suggest that the enzymes may be encoded for by the same structural gene.

Journal Article↗
PubMed1980

[Blood collection from the external jugular vein in dogs (author's transl)].

The blood samples from the cephalic vein and the external jugular vein were compared hematologically and serum biochemically in 22 beagle dogs. No significant differences (p < 0.05) between these two venous blood were observed in most of the items determined except MCV (mean corpuscular volume) and serum glucose level. The time required to collect 12 ml of blood was less than 30 seconds from the external jugular vein while two to eight minutes from the cephalic vein. From the result of this study the external jugular vein is shown more suitable region for collecting much volume of blood samples than the cephalic vein in dogs.

Animals↗
PubMed1980

[99mTc-MAA brain scan and CT in the diagnosis of intracranial diseases (author's transl)].

Brain scan with 99mTc-labelled macroaggregated albumin (MAA brain scan) and computed tomography studies (CT) were performed on 78 patients with intracranial diseases. 99mTc-MAA was injected into right and left carotid artery respectively, at the different time. And then diagnostic evaluation of both techniques were investigated. The MAA brain scan was useful for the detection of ischemic lesion in the early stage after onset of cerebral infarction. It was shown in 29.5% that partial field of anterior cerebral artery was injected through the contralateral artery. In these cases, the MAA brain scan was also useful. In the cases of hemorrhagic lesions, however, the CT was more useful than MAA brain scan. Therefore, the both techniques helped each other in the diagnosis of intracranial diseases.

Adult↗
PubMed1980

Studies on the metabolism of 1-(2-tetrahydrofuryl)-5-fluorouracil and uracil co-administered orally to tumor-bearing rats.

The metabolism of 1-(2-tetrahydrofuryl)-5-fluorouracil (FT) plus uracil (1:4, UFT) was compared with that of FT alone and that of uracil alone in tumor-bearing rats by using 3H-tracer. After oral administration of UFT, both FT and uracil were rapidly absorbed and no interaction of their absorptions was observed. The levels of 5-fluorouracil (5-FU) in the blood and tissues and the excretion of 5-FU in the urine of rats given UFT were temporarily higher, and the levels of alpha-fluoro-beta-ureidopropionic acid (a catabolic metabolite of 5-FU) in almost all tissues were lower than in rats given FT. The levels of 5-FU, 5-fluorouridine, and fluoronucleotides increased more in the tumors than in normal tissues of rats given UFT, but not in those given FT. The metabolism of uracil administered exogenously as UFT was not influenced by 5-FU or its metabolites derived from FT.

Administration, Oral↗
PubMed1980

[Early detection of recurrent uterine cervical cancers (author's transl)].

In the treatise the authors aimed to find some early signs of aggravation on the treated uterine cervical cancers by routine clinical methods. Initially nine autopsy materials died of cancer were studied to survey the anatomical spreading and each documented. Then their ante mortem findings were summarized to review. Secondarily chief complains and clinical manifestations collected above were investigated among twenty one patients who were clearly attacked and/or died of recurrence. As a control thirteen patients free from the disease were encountered. Progressive pain associated with edema either in ipsilateral low back, limbs or lower abdomen was one of the most suspicious signs of intra-pelvic recurrence. Palpable noduli and thickening of parametrial areas and pelvic walls were as well a sign of exacerbation, if they appeared after treatments. Repeating urinary infection and aggravating hydro-nephrosis et-ureter frequently implied an insidious sign of pelvic recurrence. Continuing obstinate cough may be suggestive for pulmonary metastasis and needs chest roentgenogram. Increased serum alkaline phosphatase, LDH and ESR, and decreased peripheral lymphocyte count were frequently observed in the recurrent group. A suspicious or positive vaginal cytology was mostly indicative for pathological examination that would give us a final validity. Biopsy was done for superficial enlargements.

Adult↗
PubMed1979

A case of double pylorus.

Doubling of the pyloric channel is one of the rare anomalies of the intestinal tract. There have been 23 cases of double pylorus that we have been able to find in the literature. As the etiologic aspects of double pylorus the majorities of the cases were thought to be acquired lesions from ulcer disease except three cases. We present a case of double pylorus which seems to be acquired lesion, with a review of the literature.

Aged↗
PubMed1979

The origins of unconjugated bilirubin in bile.

In order to investigate the origin of unconjugated bilirubin in bile, beta-glucuronidase activity in rat and human bile was determined at various pH. beta-Glucuronidase in rat and human bile had their optimum pH at 5.5 when phenolphthalein glucuronide and delta 1-azopigment were used as substrate, and at 6.0 when bile itself was incubated. In human and rat bile the hydrolysis was suppressed to a minimum at each physiologic pH. However, human bile shows remarkable hydrolysis in alkaline pH (7.5--8.0). On the other hand, when delta 1-azopigment was incubated in various buffers, several per cent of delta 1-azopigment were hydrolyzed non-enzymatically in neutral to alkaline pH. Thus, it was suggested that enzymatic and nonenzymatic hydrolysis contributes to the existence of unconjugated bilirubin in bile.

Animals↗
PubMed1979

Restriction map, partial cloning and localization of 9S and 12S kinetoplast RNA genes on the maxicircle component of the kinetoplast DNA of Leishmania tarentolae.

We have constructed a restriction map of the maxicircle component of the kinetoplast DNA of Leishmania tarentolae for the enzymes EcoRI, Bam HI, HaeIII, HpaII, SalI, BglII and HindIII. The 9 and 12S kinetoplast RNAs were localized on this map. Two fragments of this maxicircle molecule were cloned in the bacterial plasmid, pBR322, including a 4.4 . 10(6) dalton EcoRI/BamHI fragment which contains the 9 and 12S RNA genes.

Animals↗