Search PubMed⌕ Search

Biomedical subjects

H Manelli

Publications and source records attributed to H Manelli.

At least 19 recordsLinked to original sources
PubMed1989

Acetylcholinesterase activity in the adrenal chromaffin vesicles of Urodela.

The presence of acetylcholinesterase (AChE) activity in the adrenal chromaffin cells of Necturus maculosus and Ambystoma maculatum (Amphibia, Urodela) has been demonstrated by cytochemical method at the electron microscope level. The enzymatic activity is localized in RER and perinuclear cisternae, on the plasma membrane and within the chromaffin vesicles, both in adrenaline (A) and noradrenaline (N) cells. Moreover N cells appear to be more reactive than A cells and Necturus more reactive than Ambystoma. The possible function of the AChE activity inside the vesicles is discussed as a mechanism of protons donor or as peptidasic activity acting on various peptides present in the vesicle.

Acetylcholinesterase↗
PubMed1986

Acetylcholinesterase and pseudocholinesterases in developing chick adrenal.

The distribution of acetylcholinesterase (AChE) and pseudocholinesterases (PsChE) in chick adrenal gland during the first phases of organogenesis was studied. Acetylthiocholine iodide and butyrylthiocholine iodide were used as substrates for the two enzymes, respectively, whereas BW284c51 (1,5 bis (4-allyldimethylammonium-phenyl)pentan-3-one-dibromide) and ISO-OMPA (tetraisopropylpyrophosphoramide) were used as respective inhibitors of AchE and PsChE. AchE was present on the plasma membrane, in the perinuclear cisterna and in some cisternae of the rough endoplasmic reticulum of both interrenal and chromaffin cells; moreover enzymatic activity was found in the same sites of ganglion cells and mesenchymatic undifferentiated cells, i.e. on the inside and in the proximity of the glandular anlage. PsChE activity was localized in the perinuclear space and in the rough endoplasmic reticulum of all types of cells in the anlage. It is suggested that these enzymatic activities may be implicated in morphogenetic mechanisms.

Acetylcholinesterase↗
PubMed1985

Active cell movements and embryonic cholinesterase in the postmetamorphic completion of the adrenal morphogenesis in frogs.

The adrenal gland in Rana esculenta complex, as in other advanced anurans, is not yet in its definitive position at the end of the metamorphosis and reaches it subsequently, before sexual maturity. The displacement takes place by various means, among which active cellular movements prevail. These are demonstrated by the presence of acetylcholinesterase (AChE) in noninnervated cells (embryonic AChE). The disintegration of the connective tissues which delimit the cords of the adrenal gland, the passive transport of cells by the blood vessels and the movements of adjacent renal and mesenchymal cells collaborate with the active movements of the adrenal cells.

Acetylcholinesterase↗
PubMed1981

Comparative acid phosphatase distribution in the suprarenal gland of Discoglossus pictus, Xenopus laevis and Bufo bufo (Anurans, Amphibia).

Acid phosphatase activity was found to have a similar distribution in the suprarenal glands of Discoglossus pictus, Xenopus laevis and Bufo Bufo (Anurans, Amphibia) as determined by light and electron histochemical localization. The enzymatic activity is localized in the lysosomes of both the interrenal cells and the chromaffin cells. It is, moreover, positive on the granule membranes of the adrenaline cells whereas it appears only occasionally on the granule membranes of the noradrenaline cells. Some precipitates can also be seen occasionally at the level of the Golgi membranes.

Acid Phosphatase↗